In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Document Type : Article

Authors

Abstract

Abstract
Genomic DNA extraction with high quantity and quality is the basic necessities of molecular genetic studies. The aim of this study is the applicability of different DNA extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snail Conus coronatus, Mollusca with Phenol-chloroform, Ammonium acetate and CTAB (Cetyl trimethylammonium bromide) methods. About 30 samples were collected from Qeshm Island, Zeyton Park. After dissection on sterile condition, different kinds of Conus tissues were separated and preserved in ethanol 96%, then transferred to the lab. DNA extraction were performed from different tissues with different methods.  Spectrophotometer and electrophoresis on 1 % agarose gel were used for evaluating the quality and quantity of extracted DNA samples. According to the results, Ammonium acetate method showed sharper bands, without esmire in comparison with other extraction methods and DNA extracted from foot tissue had the best quality and quantity.

Keywords

Bandyopadhyay, PK.; Stevenson, BJ.; Cady, MT.; Olivera, BM.; Wolstenholme, DR.; (2006). Complete mitochondrial DNA sequence of a Conoidean gastropod, Lophiotoma (Xenuroturris) cerithiformis: gene order and gastropod phylogeny. Toxic; 48: 29-43.##Beacham, TD.; McIntosh, B.; Wallace, C.; (2010). A comparison of stock and individual identification for sockeye salmon (Oncorhynchus nerka) in British Columbia provided by microsatellites and single nucleotide polymorphisms. Can J Fish Aqua Sci; 67: 1274-1290.##Bernaldez, J.; López, O.; Licea, A.; Salceda, E.; Arellano, RO.; Vega, R.; Soto, E.; (2011). Electrophysiological characterization of a novel small peptide from the venom of Conus californicus that targets voltage-gated neuronal Ca2+ channels. Toxic; 57: 60-67.##Brauer, A.; Kurz, A.; Stockwell, T.; Heidler, J.; Wittig, I.; Kauferstein, S.; Mebs, D.; Stocklin, R.; Remm, M.; (2012). The Mitochondrial Genome of the Venomous Cone Snail Conus consors. Plos one; 7(12): 1-10.##De Bruyn, M.; Parenti, LR.; Carvalho, GR.; (2011). Successful extraction of DNA from archived alcohol-fixed white-eye fish specimens using an ancient DNA protocol. J Fish Biol; 78: 2074-2079.##Eschbach, E.; (2012). Ascertaining optimal protocols for DNA extraction of different qualities of pike (Esox lucius) tissue samples – a comparison of commonly used solid phase extraction methods. Env Biotech; 8 (1): 7-14##Hansen, MM.; (2002). Estimating the long-term effects of stocking domesticated trout into wild brown trout (Salmo trutta) populations: an approach using microsatellite DNA analysis of historical and contemporary samples. Mol Ecol.; 11: 1003-1015.##Hillis, DM.; Moritz, C.; (1990). Molecular taxonomy. Sinauer Associates Inc Publishers. Sunderland, Massachusetts. U .S. A. P: 120##Kaas, Q.; Westermann, J.; Craik, DJ.; (2010). Conopeptide characterization and classifications: An analysis using ConoServer. Toxic; 55(8): 1491-1509.##Lodhi, MA.; Guang-Ning, Y.; Weeden, NF.; Reisch, BI.; (1994). Simple and efficient method for DNA extraction from grapevine cultivars Vitis species Ampelopsis. Plant Mol Biol Rep; 12(1): 6-13.##Lucentini, L.; Caporali, S.; Palomba, A.; Lancioni, H.; Panara, F.; (2006a). A comparison of conservative DNA extraction methods from fins and scales of freshwater fish: a useful tool for conservation genetics. Conser. Genet; 592-613.##Lucentini, L.; Palomba, A.; Lancioni, H.; Natali, M.; Panara, F.; (2006b). A nondestructive, rapid, reliable and inexpensive method to sample, store and extract high-quality DNA from fish body mucus and buccal cells. Mol Ecol; 6: 257-260.##Olivera, BM.; (2006). Conus peptides: biodiversity-based discovery and exogenomics. j Biol Chem; 281(42): 31173-31177.##Shivji, MS.; Rogers, SO.; Stanhope, MJ.; (1992). Rapid isolation of high molecular weight DNA from marine macroalgae. Mar Ecol Prog Seri; 84: 197-203.##Wilfinger, WW.; Mackey, K.; Chomczynski, P.; (1997). Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity. BioTech; 22(3): 475-481.##