Document Type : Article
Authors
Abstract
Abstract
Pollution is a world problem with serious consequences. Heavy metals are an important group of these contaminants and enter aquatic ecosystems from anthropogenic and natural sources and animal health and systems are affected. In this study, the effect of lead exposure (3 mg/l concentration, 30 days) on activity of tissue enzymes, including acetyl cholinesterase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) In liver, gills, brain and muscle were studied in Snow trout (Schizothorax zarudnyi). The results showed that lead caused a significant inhibition of acetyl cholinesterase in the gills and brain as compared to control group (p˂0/05). The activity of ALT was increased significantly in the liver and brain homogenates following lead acetate exposure (p˂0/05). A significant increase in AST activity was observed in the liver and brain (p˂0/05). Moreover, LDH and ALP activity were increased significantly following lead acetate exposure only in the liver (p˂0/05). According to this, lead exposure in Snow trout leads to interactions between these metal and biological systems, which could affect metabolic enzyme activities in some tissues.The enzyme activity by lead did not follow the same pattern of increase or decrease and different results were based on enzymes and tissue. Therefore, monitoring of enzymatic profiles in tissues of Snow trout could be useful for identification of overall fish health and organ dysfunction following lead exposure.
Keywords
Abbas, H.H.; (2006). Acute toxicity of ammonia to common carp fingerling (Cyprinus carpio) at different pH levels. Pakistan journal of biological science; 9: 2215-2221.
Antognelli, C.; Romani, R.; Baldracchini, F.; De Santis, A.; Andreani, G.; Talesa, V.; (2003). Different activity of glyoxalase system enzymes in specimens of Sparus auratus exposed to sublethal copper concentrations. Chemico-Biological Interactions; 142: 297-305.
Atli, G.; Alptekin, O.; Tukel, S.; Canli, M.; (2006). Response of catalase activity to Ag+, Cd2+, Cr6+, Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromis niloticus. Comparative Biochemistry and Physiology; 143: 218-224.
Bainy, A. C. D.; de Medeiros, M. H. G.; Mascio, P. D.; de Almeida, E. A.; (2006). In vivo effects of metals on the acetylcholinesteras e activity of the Perna perna mussel’s digestive gland. Biotemas; 19: 35-39.
Baghshani, H.; Shahsavani, D.; (2013). Effects of lead acetate exposure on metabolic enzyme
activities in selected tissues of common carp (Cyprinus carpio). Comparative Clinical Pathology; 22: 903-907.
Beninca, C.; Ramsdorf, W.; Vicari, T.; de OliveiraRibeiro, C.A.; de Almeida, M.Z.; Silva de Assis, H.C.; (2011) Chronic genetic damages in Geophagus brasiliensis exposed to anthropic impact in Estuarine Lakes at Santa Catarina Coast–Southern of Brazil. Environmental Monitoring and Assessment; 184(4): 2045-2056.
Boge, G.; Leydet, M.; Houvet, D.; (1992). The effects of hexavalent chromium on the activity of alkaline phosphatase in the intestine of rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology; 23: 247-260.
Brewer, S.K.; Little, E.E.; De Lonay, A.J.; Beauvais, S.L.; Jones, S.B.; Ellersieck, M.R.; (2001).Behavioral dysfunctions correlate to altered physiology in rainbow trout (Oncorynchus mykiss) exposed to cholinesterase-inhibiting chemicals; 40: 70-76.
Corsi, I.; Bonacci, S.; Santovito, G.; Chiantore, M.; Castagnolo, L.; Focardi, S.; (2004). Cholinesterase activities in the Antarctic scallop Adamussium colbecki: tissue expression and effect of ZnCl2 exposure. Marine Environmental Research; 58: 401-406.
Cory-Slechta, D.; (1995). Relationships between lead-induced learning impairments and changes in dopaminergic, cholinergic, and glutamatergic neurotransmitter system functions. Annual Review of Pharmacology and Toxicology; 35: 391-415.
Dai. W.; Fu. L.; Du, H.; Jin, C.; Xu, Z.; (2009). Changes in growth performance, metabolic enzyme activities, and content of Fe, Cu, and Zn in liver and kidney of Tilapia (Oreochromis niloticus) exposed to dietary Pb. Biological Trace Element Research;128: 176-183.
Das, P.C.; Ayyappan, S.; Das, B.K.; Jena, J.K.; (2004). Nitrite toxicity in Indian major carps: sublethal effect on selected enzymes in fingerlings of Catla catla, Labeo rohita and Cirrhinos mrigala. Comparative Biochemistry and Physiology; 138: 3-10.
De La Torre, F.R.; Salibian, A.; Ferrari, L.; (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution; 109: 277-282.
De Lima, D.; Roque, G.M.; de Almeida, E.A.; (2013). In vitro and in vivo inhibition of acetylcholinesterase and carboxylesterase by metals in zebrafish (Danio rerio). Marine Environmental Research; 91: 45-51.
Ellman, G.L.; Courtney, K.D.; Andres, V. J.; (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology; 7: 88-95.
Gabriel, U.U.; Akinrotimi, O.A.; Ariweriokuma, V.S.; (2012). Changes in metabolic enzymes activities in selected organs and tissue of Clarias gariepinus exposed to cypermethrin. Chemical Engineering; 1: 25-30.
Gagnon, A.; Jumarie. C.; Hontela, A.; (2006). Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology; 78: 59-65
Gill, T.S.; Tewari, H.; Pande, J.; (1990). Use of the fish enzyme system in monitoring water quality: effects of mercury on tissue enzymes. Comparative Biochemistry and Physiology; 97: 287-292.
Gill, T.S.; Tewari, H.; Pande, J.; (1991). In vivo and in vitro effects of cadmium on selected enzymes in different organs of the fish Barbus conchonius Ham. (rosy barb). Comparative Biochemistry and Physiology; 100: 501-505.
Gioda, C.R.; Loro, V.L.; Pretto, A.; Salbego, J.; Dressler, V.; Flores, E.M.M.; (2013). Sublethal Zinc and copper exposure affect acetyl cholinesterase activity and accumulation in different tissues of Leporinus obtusidens. Bulletin of Environmental Contamination and Toxicology; 90: 12-16.
Gonzalez de Canales, M.L.; Sarasquete, M.C.; (1990). Hydrolytic enzymes of the digestive tract in clams Ruditapes decussates and Ruditapes philippinarum. Scientia Marina; 54: 89-93.
Lin, L.; Yang, Y.J.; Yang, S.S.; Leu, M.L; (1997). Aluminium utensile contribute to aluminium accumulation in patients with renal disease. American Journal of idney Diseases; 30: 653-658.
Molina, R.; Moreno, I.; Pichardo, S.; Jos, A.; Moyano, R.; Monterde, J.G.; Camean, A.; (2005). Acid and alkaline phosphatase activities and pathological changes induced in Tilapia fish (Oreochromis sp.) exposed subchronically to microcystins from toxic cyanobacterial blooms under laboratory conditions. Toxicon; 46: 725-735.
Mudipali, A.; (2007). Lead hepatotoxicity and potential health effect. Indian Journal of Medical Research; 126: 518-527.
Moussa, S.A.; Bashandy, S.A.; (2008). Biophysical and biochemical changes in the blood of rats exposed to lead toxicity. Romanian Journal of Biophysics; 18: 123-133.
Najimi, S.; Bouhaimi, A.; Daubèze, M.; Zekhnini, A.; Pellerin, J.; Narbonne, J.F.; et al.; (1997). Use of acetylcholinesterase in Perna perna and Mytilus galloprovincialis as a biomarker of pollution in Agadir marine bay (South of Morocco). Bulletin of Environmental Contamination and Toxicology; 58: 901-908.
Neff, J.M.; (1985). Use of biochemical measurements to detect pollutantmediated damage to fish. In: Cardwell RD, Purdy R, Bahner RC (eds) Aquatic Toxicology and Hazard ssessment: Seventh Symposium, Philadelphia, pp 155–183.
Nemcsok, J.; Benedezky, I.; Borors, L.; Asztalos, B.; Orbain, L.; (1981). Subcellular localiza-tion of transamination enzymes in fishes and their significance in the detection of water pollution. Acta Biologica Szegediensis; 27: 9-15
Nunes, B.; (2011). The use of cholinesterases in ecotoxicology. Reviews Environmental Contamination and Toxicology; 212: 29-59.
Oner, M.; Atli, G.; Canli, M.; (2009). Effects of metal (Ag, Cd, Cr, Cu, Zn) exposures on some enzymatic and non-enzymatic indicators in the liver of Oreochromis niloticus. Bulletin of Environmental Contamination and Toxicology; 82: 317-321.
Rainbow, P.S.; (2002). Trace metal concentrations in aquatic invertebrates: why and so what? Environmental Pollution; 120: 497-507.
Rajaei, Q.; Jahantigh, H.; Mir, A.; Hesari Motlagh, S.; Hasanpour, M.; (2012). Evaluation of concentration of heavy metals in Chahnimeh water reservoirs of Sistan-va-Baloochestan. Mazandaran University Medicine Scince; 22: 105-112.
Richetti, S.K.; Rosemberg, D.B.; Ventura-Lima, J.; Monserrat, J.M.; Bogo, M.R.; Bonan, C.D.; (2011). Acetylcholinesterase activity and antioxidant capacity of zebrafish brain is altered by heavy metal exposure. Neuro Toxicology; 32: 116-122.
Romani, R.; Antognelli, C.; Baldracchini, F.; Santis, A.; Isani, G.; Giovannini, E.; Rosi, G.; (2003). Increased acetylcholinesterase activities in specimens of Sparus auratus exposed to sublethal copper concentrations. Chemico-Biological Interactions; 145: 321-329.
Saenz, L.A.; Seibert, E.L.; Zanette, J.; Fiedler, H.D.; Curtius, A.J.; Ferreira, J.F.; (2010). Biochemical biomarkers and metals in Perna perna mussels from mariculture zones of Santa Catarina, Brazil. Ecotoxicology and environmental safety; 73: 796-804.
Shahsavani, D.; Mohri, M.; Kanani, H.G.; (2010). Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiology and Biochemistry; 36: 39-43.
Sant’Anna, M. C. B.; de Matas Soares, V.; Seibt, K.J.; Ghisleni, G.; Rico, P.E.; Rosemberg, B.D.; de Oliveira, J.R.; Schroder, N.; Bonan, C.D.; Bogo, R.M.; (2011). Iron exposure modifies acetylcholinesterase activity in zebrafish (Danio rerio) tissues: distinct susceptibility of tissues to iron overload. Fish Physiology Biochemistry; 37: 573-581.
Senger, M.R.; Seibt, K.J.; Ghisleni, G.C.; Dias, R.D.; Bogo, M.R.; Bonan, C.D.; (2011). Aluminum exposure alters behavioral parameters and increases acetylcholinesterase activity in zebrafish (Danio rerio) brain. Cell Biology and Toxicology; 27:199-205.
Toth L.; Juhasz, M.; Varga, T.; Csikkel-Szolnoki, A.; Nemcsok, J.; (1996). Some effects of CuSO4 on carp. Environmental Science and Health; 31: 627-635.
Velmurugan, B.; Selvanayagam, M.; Cengiz, E.I.; Uysal, E.; (2008). Levels of transaminases, alkaline phosphatase, and protein in tissues of Clarias gariepienus fingerlings exposed to sublethal concentrations of cadmium chloride. Environmental Toxicology; 23: 672-678.
Yildirim, M.Z.; Benli, K.C.; Selvi, M.; Ozkul, A.; Erko, F.; Kocak, O.; (2006). Acute toxicity behavioural changes and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile Tilapia (Oreochromis niloticus) fingerlings. Environmental Toxicology; 21: 614-620.
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