Experimental animal Biology

In collaboration with Payame Noor University and Iranian Society of Physiology and Pharmacology

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Evaluation of Biochemical Parameters in Sheep after Placement of Tissue Engineered Valve in Thoracic Aorta

Document Type : Article

Authors

1 Assistant Professor and Professor, Department of Internal medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran

2 Assistant Professor and Professor, Department of Internal medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran,

3 Professor, Department of Urology, Pediatric Urology Research center, Children Hospital Medical Center, Tehran University of Medical Sciences, Tehran

4 Associate Professor, Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran 5. Professor, Tehran heart center, Tehran University of Medical Sciences, Tehran

5 Professor, Tehran heart center, Tehran University of Medical Sciences, Tehran

6 Assistant Professor, Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran

7 Post Graduate Students, Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran

Abstract

Valvular diseases are a common problem and valvular replacement is usual treatment for valvular destruction. Tissue engineered heart valve has some advantages, that make them an interesting alternative for previous valves. The aim of this study is measurement of biochemical factors for evaluation of short term efficacy of tissue engineered heart valve. This study was undertaken on 5 male sheep with average weight of 45-55 kg. They have been of-feed 24 hours before of operation. The tissue engineered heart valves were placed in thoracic aorta and after surgery they connected to ventilator under SIMV mode. The arterial blood sample Were collected every hours for 12 hours and evaluated, PCO2, PO2, tCO2, SO2, Hct, Na+ and K+. Arterial blood sample has been taken from auricularis artery. Blood pH fell down in all sheep after surgery but in two sheep as a result of sever blood loos during surgery pH went up toward acidosis, finally after 12 hours pH in all sheep was in normal range. After operation PCO2 was low an PO2 was high because of mechanical ventilation. All the factors backed to normal range after 12 hours of operation. This study shows that biochemical factors had fluctuation in first 12 hours but after 12 hours most of them backed to normal range.
 

Keywords

References
Elkins RC, Dawson P, Goldstein S, Walsh P, Black KS (2001) Decellularized human valve allografts. Ann Thorac Surg. 71: S 428-S 432.
Fuchs JR, Nasseri BA, Vacanti  JP (2001) Tissue engineering: a 21st century solution to surgical reconstruction. Ann Thorac Surg. 72: 577-591.
Hoerstrup SP, Sodian R, Daebritz S, Wang J, Bacha EA, Martin DP, Moran AM, Guleserian KJ, Sperling JS, Kaushal S, Vacanti JP, Schoen FJ, Mayer JE (2000) Functional living trileaflet heart valves grown in vitro. Circulation. 102: III 44- III 49.
Lange RWJ, Homann M (2001) Performance of allografts and xenografts for right ventricular outflow tract reconstruction. Ann Thorac Surg. 71: S 365-S 367.
North RA, Stewart AW (1999) Long-term survival and valverelated complications in young women with cardiac valve replacements. Circulation. 99: 2669-2676.
Opitz F, Schenke-Layland K, Cohnert TU, Starcher B, Halbhuber KJ, Martin, DP, Stock U (2004) Tissue engineering of aortic tissue: dire consequence of dub optimal elastic fiber synthesis in vivo. cardiac res. 63: 719-730.
Sacks MS, Schoen FS (2002) Collagen fiber disruption occurs independent ofcalcification in clinically explanted bioprosthetic heart valves. J BiomeMater Res. 69: 351-372.
Schoen FJ (2008) Evolving Concepts of Cardiac Valve Dynamics: The Continuum of Development, Functional Structure, Pathobiology, and Tissue Engineering. Circulation. 118: 1864-1880.
Serghei Cebotari HM, Kallenbach K, Kostin S, Repin O, Batrinac A, Kleczka C, Ciubotaru A, Haverich A (2002) Construction of Autologous Human Heart Valves Based on an Acellular Allograft Matrix. Circulation. 106: 163-168.
Simon P, Kasimir MT, Seebacher, G, Weigel G, Ullrich R, Salzer-Muhar U, Rieder E, Wolner E (2003) Early failure of the tissue engineered porcine heart valve SYNERGRAFTe in pediatric patients. European Journal of Cardio-thoracic Surgery. 23: 1002-1006.
Steinhoff G, Stock U, Karim N, Mertsching H, Timke A, Meliss RR, Pethig K, Haverich A, Bader A (2000) Tissue engineering of pulmonary heartvalves on allogeneic acellular matrix conduits. Circulation. 102: III50-III55.
Vrandecic MFB, Fantini F, Barbosa J, Martins I, Cesar deOliveira O, Martins C, Max R, Drumond L, Oliveira C, Ferrufino A, Alcocer E, Silva JA, Vrandecic E (1998) The use of bovine pericardium forpulmonary valve reconstruction or conduit replacement: long-termclinical follow up. J Heart Valve Dis. 7: 54-61.
Yankah AC, Klose H, Petzina R (2002) Surgical management of acute aortic root endocarditis with viable homograft: 13-year experience. Eur J Cardiothorac Surg. 21: 260-267.
Experimental animal Biology
Volume 3, Issue 2 - Serial Number 2
March 2015
Pages 31-37
Files
Share
How to cite
Statistics