Document Type : Article
Authors
Abstract
Abstract
The Saffron plant (Crocus sativus), a native plant from Iran and especially of Khorasan region, has a specific place for people's diet. According to several studies about effects of Saffron and because there are no specific studies on the effects of Saffron on the development of Drosophila melanogaster as an animal model, this subject is being evaluated in the current study. A total of 5 pairs of 3-days-old wild Drosophila melanogaster were transferred to every culture plate containing different concentrations of Saffron in order to intercross and oviposition and were brought out after 8 hrs. sampling of larvae were performed in the seven steps at intervals of twelve hours and in each step, Morphometrical changes in the length and width of the larvae, were studied. The obtained data were evaluated statistically using SAS software, The variance between groups were evaluated by the test of One way ANOVA, and the mean of data were compared using Ttukey test with minimum significance level of p in high concentrations, has inverse effect on the biology of this insect.
Keywords
Abdullaev, FI.; (2002) Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L.). Exp. Biol Med. 227: 20.
Abdullaev, FI.; (2003) Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicology in vitro, 17(5-6):751.
Abdullaev, FI.; Gonzalez, D.; Mejia, E.; (1995) Inhibition of colony formation of Hela cells by naturally occurring and synthetic agents. Biofactors, 96; 5(3): 133-38.
Aung, HH.; Wang, CZ.; Ni, M.; Fishbein, A.; et al. (2007) Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells. Exp Oncol, 29(3): 175-180.
Castaneda, PL.; Munoz, GLE,; Duran, DA.; Heres, PME.; Duenas, GIE.; (2001) LD50 in Drosophila melanogaster fed on lead nitrate and lead acetat. Drosophila Information Service, 84: 44-48.
Das, I.; Das, S.; Saha, T.; (2010) Saffron suppresses oxidative stress in DMBA- induced skin carcinoma, A histopathological study. Acta histochemic. a; 112: 317-327.
Day, DM.; Wallman, JF.; (2006) Width as an alternative measurement to length for post-mortem interval estimatios using Calliphora augur (Diptera: Calliphoridae) larvae. Forensic Science International, 159: 158-167.
Ding, L.; Wang, Y.; (2006) Effect of copper on the development, protein and esterase isozymes of Drosophila melanogaster. Integrative Zoology, 2:
73-77.
Escribano, J.; Diaz-Guerra, JMM.; Riese, HH.; Ontanon, J.; et al. (1999) In vitro activation of macrophages by a novel proteoglycan isolated from corms of Crocus sativus L. Cancer Letters, 144: 107-114.
Fatehi, M.; Rashidabady, T.; Fatehi-Hassanabad, Z.; (2003) Effects of Crocus sativus petals’extract on rat blood pressure and on responses induced by electrical field stimulation in the rat isolated vas deferens and guinea-pig ileum. Journal of Ethnopharmacology, 84: 199-203.
Gout, B.; Bourges, C.; Dubreuil, SP.; (2010) Satiereal, a Crocus sativus L., extract, reduces snacking and increases satiety in a randomized placebo-controlled study of mildly overweight, healthy women. Nutrition Research, 30: 305-313.
Goyal, SN.; Arora, S.; Sharma, AK.; Joshi, S.; et al. (2010) Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultra structural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine, 17: 227-232.
Hirsch, HVB.; Mercer, J.; Sambaziotis, H.; Huber, M.; et al. (2003) Behavioral effects of chronic exposureti low levels of lead in Drosophila melanogaster. Neuro Toxicology, 24: 435-442.
Hosseini, SM.; Dashti, MH.; Anvari, M.; Zeinali, F.; et al. (2009) Studying teratogenic and abortificant effects of different doses of saffron (CrocusSativus) decoction in 1st or 2nd trimesters in mice. Iranian Journal of Reproductive Medicine, 7: 2- 35.
Hosseinzadeh, H.; Younesi, HM.; (2002) Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacology, 2: 147-155.
Hosseinzadeh, H.; Ziaee, T.; Sadeghi, A.; (2008) The effect of saffron, Crocus sativus stigma, extract and its constituents, safranal and crocin on sexual behaviors in normal male rats. Phytomedicine, 15: 491-495.
Nezhad Shahrokhabadi, KH.; Tavakol Afshari, J.; Rakhshandeh, H.; Borouk, A.; (2009) Study of cytotoxicity effect of total saffron extract on hepatocarcinoma cell line (hepg2). Medical sciences journal of Islamic Azad University, 19(3)(57): 154-159.
Pitsikas, N.; Boultadakis, A.; Gergiadou, G.; Tarantilis, PA.; et al. (2008) Effects of the active constituents of Crocus sativus L. in an animal model of anxiety. Phytomedicine, 15: 1135-1139.
Premkumar, K.; (2003) Protective effects of saffron (Crocus sativus L.) on genotoxininduced oxidative stress in Swiss albino mice. Phytother Res, 17(6): 614-617.
Premkumar, K.; Thirunavukkarasu, C.; Abraham, SK.; Santhiya, ST.; et al. (2006) Protective effect of saffron (Crocus sativus L.) aqueous extract against genetic damage induced by anti-tumor agents in mice. Hum ExpToxicol, 25(2): 79-84.
Salomi, MJ.; Nair, SC.; Panikkar, KR.; (1991) Inhibitory effects of Nigella sativa and saffron (Crocus sativus) on chemical carcinogenesis in mice. Nutr Cancer, 16 (1): 67-72.
Tafazoli, M.; Kermani, T.; Saadatjoo, AR.; (2004) Effects of saffron on abortion and its side effect on mice balb/c. Ofogh-e- danesh, Jornals of University of Medical Sciences and Health Services, 10(3): 52-55.
Takashi, O.; Hiroshi, S.; Ken-ichi, M.; Katsunori, I.; et al. (2007) Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo. Biochimica ET Biophysica Acta, 1770: 578-584.
Tickoo, S.; Russell, S.; (2002) Drosophila melanogaster as a model system for drug discovery and pathway screening. Current Opinion in Pharmacology, 2: 555-560.
Abdullaev, FI.; (2003) Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicology in vitro, 17(5-6):751.
Abdullaev, FI.; Gonzalez, D.; Mejia, E.; (1995) Inhibition of colony formation of Hela cells by naturally occurring and synthetic agents. Biofactors, 96; 5(3): 133-38.
Aung, HH.; Wang, CZ.; Ni, M.; Fishbein, A.; et al. (2007) Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells. Exp Oncol, 29(3): 175-180.
Castaneda, PL.; Munoz, GLE,; Duran, DA.; Heres, PME.; Duenas, GIE.; (2001) LD50 in Drosophila melanogaster fed on lead nitrate and lead acetat. Drosophila Information Service, 84: 44-48.
Das, I.; Das, S.; Saha, T.; (2010) Saffron suppresses oxidative stress in DMBA- induced skin carcinoma, A histopathological study. Acta histochemic. a; 112: 317-327.
Day, DM.; Wallman, JF.; (2006) Width as an alternative measurement to length for post-mortem interval estimatios using Calliphora augur (Diptera: Calliphoridae) larvae. Forensic Science International, 159: 158-167.
Ding, L.; Wang, Y.; (2006) Effect of copper on the development, protein and esterase isozymes of Drosophila melanogaster. Integrative Zoology, 2:
73-77.
Escribano, J.; Diaz-Guerra, JMM.; Riese, HH.; Ontanon, J.; et al. (1999) In vitro activation of macrophages by a novel proteoglycan isolated from corms of Crocus sativus L. Cancer Letters, 144: 107-114.
Fatehi, M.; Rashidabady, T.; Fatehi-Hassanabad, Z.; (2003) Effects of Crocus sativus petals’extract on rat blood pressure and on responses induced by electrical field stimulation in the rat isolated vas deferens and guinea-pig ileum. Journal of Ethnopharmacology, 84: 199-203.
Gout, B.; Bourges, C.; Dubreuil, SP.; (2010) Satiereal, a Crocus sativus L., extract, reduces snacking and increases satiety in a randomized placebo-controlled study of mildly overweight, healthy women. Nutrition Research, 30: 305-313.
Goyal, SN.; Arora, S.; Sharma, AK.; Joshi, S.; et al. (2010) Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultra structural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine, 17: 227-232.
Hirsch, HVB.; Mercer, J.; Sambaziotis, H.; Huber, M.; et al. (2003) Behavioral effects of chronic exposureti low levels of lead in Drosophila melanogaster. Neuro Toxicology, 24: 435-442.
Hosseini, SM.; Dashti, MH.; Anvari, M.; Zeinali, F.; et al. (2009) Studying teratogenic and abortificant effects of different doses of saffron (CrocusSativus) decoction in 1st or 2nd trimesters in mice. Iranian Journal of Reproductive Medicine, 7: 2- 35.
Hosseinzadeh, H.; Younesi, HM.; (2002) Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacology, 2: 147-155.
Hosseinzadeh, H.; Ziaee, T.; Sadeghi, A.; (2008) The effect of saffron, Crocus sativus stigma, extract and its constituents, safranal and crocin on sexual behaviors in normal male rats. Phytomedicine, 15: 491-495.
Nezhad Shahrokhabadi, KH.; Tavakol Afshari, J.; Rakhshandeh, H.; Borouk, A.; (2009) Study of cytotoxicity effect of total saffron extract on hepatocarcinoma cell line (hepg2). Medical sciences journal of Islamic Azad University, 19(3)(57): 154-159.
Pitsikas, N.; Boultadakis, A.; Gergiadou, G.; Tarantilis, PA.; et al. (2008) Effects of the active constituents of Crocus sativus L. in an animal model of anxiety. Phytomedicine, 15: 1135-1139.
Premkumar, K.; (2003) Protective effects of saffron (Crocus sativus L.) on genotoxininduced oxidative stress in Swiss albino mice. Phytother Res, 17(6): 614-617.
Premkumar, K.; Thirunavukkarasu, C.; Abraham, SK.; Santhiya, ST.; et al. (2006) Protective effect of saffron (Crocus sativus L.) aqueous extract against genetic damage induced by anti-tumor agents in mice. Hum ExpToxicol, 25(2): 79-84.
Salomi, MJ.; Nair, SC.; Panikkar, KR.; (1991) Inhibitory effects of Nigella sativa and saffron (Crocus sativus) on chemical carcinogenesis in mice. Nutr Cancer, 16 (1): 67-72.
Tafazoli, M.; Kermani, T.; Saadatjoo, AR.; (2004) Effects of saffron on abortion and its side effect on mice balb/c. Ofogh-e- danesh, Jornals of University of Medical Sciences and Health Services, 10(3): 52-55.
Takashi, O.; Hiroshi, S.; Ken-ichi, M.; Katsunori, I.; et al. (2007) Protective effects of carotenoids from saffron on neuronal injury in vitro and in vivo. Biochimica ET Biophysica Acta, 1770: 578-584.
Tickoo, S.; Russell, S.; (2002) Drosophila melanogaster as a model system for drug discovery and pathway screening. Current Opinion in Pharmacology, 2: 555-560.
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