Document Type : Article
Authors
Abstract
Despite the application of Marsh frog Rana (Pelophylax) ridibunda in research, there is little information about the biology of different populations of this frog, especially in southern Iran Provinces. The first aim of this study was to investigate differences in biometric characteristics especially between male and female from different populations of species in Fars province. The second aim was the histological study on the male and female gonads which can help to explore some reproductive strategies of the frog in this region (Fars Province). This study will determine whether at the time of spawning, the gonads(testies and ovaries) are completely depleted from egg and sperm. In addition to this, the possibility of guessing the ability of the marsh frogs to spawn more than once during the reproductive season will be investigated. For this purpose 120 samples (87 females and 33 annuly males) from different population areas (Ghadamgah, Mehkoyeh, Kohmareh Sorkhi and Pol Berenjie) were collected. Samples were fixed in formaldehyde and transferred to the laboratory. Morphometric measurements were made and samples labeled. For histological study of gonads, adult pairs in amplexuses situation were collected from the natural breeding sites. One-way analysis of variance (ANOVA) showed that the samples from four population sites were significantly different (P
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