Synergistic effect of selenium and calcium nanoparticles on the ‎half-life of alkaline serine protease enzyme

Emami Nejad, Asieh; Fazilati, Mohammad; Salavati, Hossein; Nazem, Habiballah

doi:10.30473/eab.2024.71266.1951

Document Type : Article

Authors

Department of Biology, Payam Noor ‎University (PNU), Tehran, ‎Iran‎

10.30473/eab.2024.71266.1951

Abstract

The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine protease enzymes using selenium and calcium nanoparticles was presented. Due to its high biocompatibility, toxicity, and low cost, these nanoparticles have become one of the most prominent non-metallic oxide nanoparticles, which, with their antioxidant and anti-inflammatory properties, can protect against the destruction of enzymes by free radicals. The results showed that these nanoparticles can significantly increase the half-life of these enzymes. On the other hand, the serine protease enzyme immobilized on selenium nanoparticles has unique biochemical characteristics in the presence and absence of calcium. The obtained results indicate that the stabilized enzyme has high activity and stability in a wide range of temperatures and pH compared to pure and impure-free enzyme. The results showed that the enzyme immobilized on selenium nanoparticles increased its activity by more than 99% in the presence of calcium at the temperature of 45°C. On the other hand, the half-life of the free enzyme increased from 95 minutes in the pure form to 115 minutes in the form immobilized on nanoparticles in the presence of calcium. These results indicate that the presence of nanoparticles can prevent the destruction of enzymes by free radicals.

Keywords

Main Subjects

Biochemistry

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Experimental animal Biology

Synergistic effect of selenium and calcium nanoparticles on the ‎half-life of alkaline serine protease enzyme

References

References

Volume 12, Issue 4 - Serial Number 48
May 2024
Pages 65-76

Synergistic effect of selenium and calcium nanoparticles on the ‎half-life of alkaline serine protease enzyme

References

References

Volume 12, Issue 4 - Serial Number 48May 2024Pages 65-76

Volume 12, Issue 4 - Serial Number 48
May 2024
Pages 65-76