Document Type : Article
Author
Department of Biology, Islamic Azad University, Marand Branch Iran
10.30473/eab.2024.70052.1933
Abstract
Alzheimer's disease is one of the most common neurodegenerative diseases. Beta-amyloid plaques and neurofibrillary tangles are two important factors in Alzheimer's disease. Many cellular changes, such as oxidative stress, neuronal inflammation, and mitochondrial disorders are also seen in Alzheimer's disease, which lead to neuronal death. Kaempferol, one of the plant flavonoids derived from the chamomile plant, has important effects on disorders related to brain diseases such as Alzheimer's disease due to the production of antioxidants.In this study, the effects of chamomile plant flavonoids on memory disorders in Alzheimer's rats were studied. For prepare and purify kaempferol, the hydroalcoholic extract of the collected chamomiles, and quantitative-qualitative analysis of the chemical substances of the chamomile extract using gas chromatograph connected to mass spectrometer (GC/MS) method were used. In this study, we used of 56 adult male rats were divided into 7 groups including control - vehicle 1 (kaempferol solvent) - vehicle 2 (streptozotocin drug solvent) - Alzheimer's - kaempferol 120mg/kg - kaempferol 250mg/kg - kaempferol 400mg/kg. Diabetes was induced by injecting a single dose of 60 mg/kg streptozotocin intraperitoneally. Kaempferol were administered for 15 days. The shuttle box device was used to measure memory and learning, and the delay time in the shuttle box was recorded. The results were analyzed by SPSS 22 software, ANOVA and Tokay tests. The significance of the data difference was considered at the p ≤ 0.05 levels. The results of the data analysis showed that the doses of 400 mg/kg and 250 mg/kg of kaempferol of chamomile plant significantly increased the delay time in entering the dark section at p≤0.001 compared to the control group and improved spatial memory in rats. Which does this effect is probably due to the presence of compounds such as quercetin and phytoestrogens. Also, oxidative stress parameters were significantly reduced in Alzheimer's groups treated with chamomile flavonoids (P < 0.001) . The results of the experiments show that plant flavonoids are able to restore the spatial memory and oxidative stress parameters of the groups treated with streptozotocin to normal levels.
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