Document Type : Article
Author
Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran
10.30473/eab.2023.68141.1912
Abstract
In this study, rainbow trout diploid, triploid, and tetraploid were comparatively investigated regarding the growth performance, survival rate, and erythrocyte characteristics. For the triploid production, the fertilized eggs received 26.5 ℃ thermal shock under three treatments: 1. double 1-min shock after 15 and 30 min of the fertilization, 2. one 15-min shock after 15 min of the fertilization, and 3. one 12-min shock after 20 min of the fertilization. Tetraploidy was induced by application of 28 ℃ thermal shock under three treatments: 1. 10-min shock after 59 degree-hours of the fertilization, 2. 10-min shock after 66 degree-hours of the fertilization, and 3. 10-min shock after 72 degree-hours of the fertilization. The best triploidization results, 66.6% survival rate and 87% triploidization rate, achieved by application of 12-min shock after 20 min of the fertilization. The best tetraploidization results, 54.9% survival rate and 7.94% triploidization rate, achieved by application of 10-min shock after 59 degree-hours of the fertilization. There were no significant differences between the triploid and diploid fish, wheras the tetraploids showed significantly lower growth rate than the diploids. In conclusion, in this study, triploid and tetraploid production of rainbow trout were improved, and application of erythrocyte cell size as a reliable and efficient polyploidy detection method was emphasized.
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Main Subjects
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