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Investigating the Effect of Ascorbic Acid on the Viability of ‎Mesenchymal ‎Stem Cell Cultured on the Skeletal Muscle ‎Decellularized Scaffold

Document Type : Article

Authors

1 Department of Animal Sciences and ‎Marine Biology, Faculty of Life ‎Sciences and Biotechnology, Shahid ‎Beheshti University, Tehran, Iran

2 ‎Department of Biology, Faculty of ‎Basic Science, Ale Taha Institute of ‎Higher Education, Tehran, Iran‎

10.30473/eab.2023.68207.1913

Abstract

Tissue engineering is an emerging field based on the three elements of cells, scaffolds, and bioactive molecules, and can be a useful method for treating muscle injuries. The aim of this study is to investigate the effect of ascorbic acid (AA) on the viability of bone marrow mesenchymal stem cells (BM-MSCs) cultured on skeletal muscle decellularization scaffold. First, BM-MSCs were extracted from rat leg bone marrow and cultured in vitro. The identity of the cells was assessed using flow cytometry. The extracted rat skeletal muscle was decellularized using a 1% SDS solution. The decellularization process was investigated by Masson Trichrome, and Alcian blue and DAPI staining.BM-MSCs were cultured on decellularized scaffolds and treated with 1 mM AA for 2 days. Then, the survival and viability of the cells were evaluated using scanning electron microscope (SEM) and MTT methods, respectively.BM-MSCs had a spindle morphology, and the results of flow cytometry showed the expression of CD44 and CD90 and the lack of expression of CD45 and CD34 in more than 90% of the cells. The staining verified the preservation of collagen and glycosaminoglycans and the absence of DNA in the decellularized tissue. MTT results showed that AA significantly increases the viability of BM-MSCs (P<0.05). Also, the SEM results showed that the cells in the group treated with AA were more proliferated. In general, AA can improve the efficiency of muscle tissue engineering by increasing the viability of BM-MSCs.

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References
Blake, S. (2008). Vitamin C: The Citrus Antioxidant. Vitamins and Minerals Demystified, 55-71.
Chen, Z., Jin, M., He, H., Dong, J., Li, J., Nie, J., ... Wu, F. (2023). Mesenchymal stem cells and macrophages and their interactions in tendon-bone healing. Journal of Orthopaedic Translation, 39, 63-73.
Choi, K.-M., Seo, Y.-K., Yoon, H.-H., Song, K.-Y., Kwon, S.-Y., Lee, H.-S., & Park, J.-K. (2008). Effect of ascorbic acid on bone marrow-derived mesenchymal stem cell proliferation and differentiation. Journal of bioscience and bioengineering, 105(6), 586-594.
Cittadella Vigodarzere, G., & Mantero, S. (2014). Skeletal muscle tissue engineering: strategies for volumetric constructs. Frontiers in physiology, 5, 362.
Escobar, L. M., Escobar, J. D., Bendahan, Z., & Castellanos, J. E. (2021). Retinoic and ascorbic acids induce osteoblast differentiation from human dental pulp mesenchymal stem cells. Journal of Oral Biology and Craniofacial Research, 11(2), 143-148.
Fujisawa, K., Hara, K., Takami, T., Okada, S., Matsumoto, T., Yamamoto, N., & Sakaida, I. (2018). Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells. Stem cell research & therapy, 9(1), 1-12.
Gilbert-Honick, J., Ginn, B., Zhang, Y., Salehi, S., Wagner, K. R., Mao, H.-Q., & Grayson, W. L. (2018). Adipose-derived stem/stromal cells on electrospun fibrin microfiber bundles enable moderate muscle reconstruction in a volumetric muscle loss model. Cell Transplantation, 27(11), 1644-1656.
Han, C., Wang, R., Xu, N., Wei, X., Wei, Q., & Xu, X. (2022). Visual analysis of mesenchymal stem cell research in liver disease based on bibliometrics. iLIVER, 1(4), 283-291.
Hematti, P. (2011). Human embryonic stem cell–derived mesenchymal stromal cells. Transfusion, 51, 138S-144S.
Jiwlawat, N., Lynch, E., Jeffrey, J., Van Dyke, J. M., & Suzuki, M. (2018). Current progress and challenges for skeletal muscle differentiation from human pluripotent stem cells using transgene-free approaches. Stem Cells International, 2018.
Khanban, H., Fattahi, E., & Talkhabi, M. (2019). In vivo administration of G9a inhibitor A366 decreases osteogenic potential of bone marrow-derived mesenchymal stem cells. Excli journal, 18, 300.
Langer, R., & Peppas, N. A. (2003). Advances in biomaterials, drug delivery, and bionanotechnology. AIChE Journal, 49(12), 2990-3006.
Li, C.-J., Sun, L.-Y., & Pang, C.-Y. (2015). Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis. Scientific Reports, 5(1), 9819.
Lindblad, M., Tveden-Nyborg, P., & Lykkesfeldt, J. (2013). Regulation of vitamin C homeostasis during deficiency. Nutrients, 5(8), 2860-2879.
Nakamura, Y., Miyaki, S., Ishitobi, H., Matsuyama, S., Nakasa, T., Kamei, N., . . . Ochi, M. (2015). Mesenchymal‐stem‐cell‐derived exosomes accelerate skeletal muscle regeneration. FEBS letters, 589(11), 1257-1265.
Narciso, M., Ulldemolins, A., Junior, C., Otero, J., Navajas, D., Farre, R., ... & Almendros, I. (2022). Novel decellularization method for tissue slices. Frontiers in Bioengineering and Biotechnology, 10, 832178.
Narita, Y., Yamawaki, A., Kagami, H., Ueda, M., & Ueda, Y. (2008). Effects of transforming growth factor-beta 1 and ascorbic acid on differentiation of human bone-marrow-derived mesenchymal stem cells into smooth muscle cell lineage. Cell and tissue research, 333(3), 449-459.
Qazi, T. H., Duda, G. N., Ort, M. J., Perka, C., Geissler, S., & Winkler, T. (2019). Cell therapy to improve regeneration of skeletal muscle injuries. Journal of Cachexia, Sarcopenia and Muscle, 10(3), 501-516.
Qiu, X., Liu, S., Zhang, H., Zhu, B., Su, Y., Zheng, C., . . . Zhao, X. (2018). Mesenchymal stem cells and extracellular matrix scaffold promote muscle regeneration by synergistically regulating macrophage polarization toward the M2 phenotype. Stem cell research & therapy, 9, 1-15.
Raman, N., Imran, S. A., Noordin, K. B. A. A., Zaman, W. S. W. K., & Nordin, F. (2022). Mechanotransduction of mesenchymal stem cells (MSCs) during cardiomyocytes differentiation. Heliyon.
Sam Daliri, F., Talkhabi, M., Toolabi, N., Attari, F., & Kehtari, M. (2023). Investigation of the Effect of Conditioned Media of Mesenchymal Stem Cells Treated with Ascorbic Acid on Proliferative Behavior of Breast Cancer Cells. Journal of Animal Biology, 15(2), 233-245.
Savini, I., Catani, M. V., Duranti, G., Ceci, R., Sabatini, S., & Avigliano, L. (2005). Vitamin C homeostasis in skeletal muscle cells. Free radical biology and medicine, 38(7), 898-907.
Stevens, M. M., & George, J. H. (2005). Exploring and engineering the cell surface interface. Science, 310(5751), 1135-1138.
Takaku, Y., Ito, K., Chida, D., & Sato, T. (2022). Vascular endothelial growth factor-D upregulation in mesenchymal stem cells derived from melanocortin 2 receptor-deficient mice during osteoblast differentiation. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 34(6), 679-682.
Talaei-Khozani, T., & Yaghoubi, A. (2022). An overview of post transplantation events of decellularized scaffolds. Transplant Immunology, 74, 101640.
Talkhabi, M., Pahlavan, S., Aghdami, N., & Baharvand, H. (2015). Ascorbic acid promotes the direct conversion of mouse fibroblasts into beating cardiomyocytes. Biochemical and Biophysical Research Communications, 463(4), 699-705.
Talovic, M., Patel, K., Schwartz, M., Madsen, J., & Garg, K. (2019). Decellularized extracellular matrix gelloids support mesenchymal stem cell growth and function in vitro. Journal of tissue engineering and regenerative medicine, 13(10), 1830-1842.
Urciuolo, A., & De Coppi, P. (2018). Decellularized tissue for muscle regeneration. International Journal of Molecular Sciences, 19(8), 2392.
VanDusen, K. W., Syverud, B. C., Williams, M. L., Lee, J. D., & Larkin, L. M. (2014). Engineered skeletal muscle units for repair of volumetric muscle loss in the tibialis anterior muscle of a rat. Tissue Engineering Part A, 20(21-22), 2920-2930.
Wahyuningsih, K. A., Karina, K., Rosadi, I., Rosliana, I., & Subroto, W. R. (2020). Effect of ascorbic acid on morphology of post-thawed human adipose-derived stem cells. Stem Cell Investigation, 7.
Wang, Y., Singh, A., Xu, P., Pindrus, M. A., Blasioli, D. J., & Kaplan, D. L. (2006). Expansion and osteogenic differentiation of bone marrow-derived mesenchymal stem cells on a vitamin C functionalized polymer. Biomaterials, 27(17), 3265-3273.
Yarlagadda, P. K., Chandrasekharan, M., & Shyan, J. Y. M. (2005). Recent advances and current developments in tissue scaffolding. Bio-medical materials and engineering, 15(3), 159-177.
Experimental animal Biology
Volume 12, Issue 1 - Serial Number 45
No.1
September 2023
Pages 57-66
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