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The presence of Interleukin 35 in seminal plasma: is it a ‎significant ‎issue in male infertility? ‎

Document Type : Article

Authors

1 Department of Reproductive Biology, ‎School of Advanced ‎Medical Sciences ‎and Technologies, Shiraz University ‎of Medical Sciences, ‎Shiraz, Iran ‎

2 Stem Cell Technology Research ‎Center, Shiraz University of ‎Medical ‎Sciences, Shiraz, Iran‎

3 ‎Department of Reproductive ‎Biology, School of Advanced ‎Medical ‎Sciences and Technologies, ‎Shiraz University of Medical ‎Sciences, Shiraz, Iran

4 ‎Department of Obstetrics and ‎Gynecology, School of Medicine, ‎Shiraz ‎University of Medical ‎Sciences, Shiraz, Iran‎

5 ‎Department of Obstetrics and ‎Gynecology, School of Medicine, ‎Shiraz ‎University of Medical ‎Sciences, Shiraz, Iran

6 Infertility Research ‎Center, Zeynab Hospital, ‎Shiraz ‎University of Medical Sciences, ‎Shiraz, Iran‎

7 Department of Obstetrics and ‎Gynecology, Mousavi Hospital,‎‏ ‏‎School of Medicine, Zanjan ‎University of Medical Sciences, ‎Zanjan, Iran‎

10.30473/eab.2023.68036.1911

Abstract

Approximately half of infertility in couples can be attributed to male factors, which may occur in a wide range of causes. Examining seminal plasma parameters is the initial and simplest approach in male infertility evaluation and provides valuable information about fertility status. In this study, the presence and relationship between the concentration of interleukin 35 (IL-35) in seminal plasma and standard parameters of sperm in infertile men were investigated. Semen analysis was performed on semen samples of men referred to the Infertility Center of Shiraz University of Medical Sciences. Semen samples were analyzed in accordance with the laboratory guidelines of the World Health Organization and were divided into three groups including 21 normozoospermic, 21 asthenoteratozoospermic, and 15 azoospermic samples. The seminal concentration level of IL-35 was analyzed using the enzyme-linked immunosorbent assay (ELISA) method. Results showed that IL-35 was detectable with different levels in the seminal plasma of studied groups, however, its concentration did not show a statistically significant difference (p>0.05). The correlation analysis between IL-35 concentration and sperm standard parameters showed that there is a significant negative correlation between IL-35 concentration and the normal sperm morphology percentage (r=0.317, p=0.04), while no significant correlation was observed with sperm count (r=-0.27, p=0.08) and sperm motility (r=-0.27, p=0.08). Despite the presence of IL-35 in semen, even in normozoospermic male patients, its role and functional significance have not been determined. The existence of a negative relationship between the IL-35 concentration and the normal sperm morphology percentage can indicate its importance in male infertility status.

Keywords

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References
Al-hadrawi, K. K., ALGarawy, R. T., & Darweesh, M. F. (2022). The Impact of IL-35, Bacterial Prostatitis in Development Male Infertility in Najaf Province Patients. The Egyptian Journal of Hospital Medicine, 89(1), 4278-4283.
Archana, S. S., Selvaraju, S., Binsila, B. K., Arangasamy, A., & Krawetz, S. A. (2019). Immune regulatory molecules as modifiers of semen and fertility: A review. Molecular reproduction and development, 86(11), 1485-1504.
Azad, M., Keshtgar, S., Jahromi, B. N., Kanannejad, Z., & Gharesi-Fard, B. (2017, Dec). T helper cell subsets and related cytokines in infertile women undergoing in vitro fertilization before and after seminal plasma exposure. Clin Exp Reprod Med, 44(4), 214-223. https://doi.org/10.5653/cerm.2017.44.4.214
Babakhanzadeh, E., Nazari, M., Ghasemifar, S., & Khodadadian, A. (2020). Some of the Factors Involved in Male Infertility: A Prospective Review. Int J Gen Med, 13, 29-41. https://doi.org/10.2147/ijgm.S241099
Babinets, L. S., Migenko, B. O., Borovyk, I. O., Halabitska, I. M., Lobanets, N. V., & Onyskiv, O. O. (2020). The role of cytocin imbalance in the development of man infertility. Wiad Lek, 73(3), 525-528.
Bello, R. O., Chin, V. K., Abd Rachman Isnadi, M. F., Abd Majid, R., Atmadini Abdullah, M., Lee, T. Y., Amiruddin Zakaria, Z., Hussain, M. K., & Basir, R. (2018). The role, involvement and function (s) of interleukin-35 and interleukin-37 in disease pathogenesis. International Journal of Molecular Sciences, 19(4), 1149.
Bukharin, O., Kuzmin, M., Perunova, N., Nikiforov, I., Chainikova, I., & Ivanova, E. (2022). Diagnostic cytokine marker of male infertility-interleukin 4. Klinicheskaia Laboratornaia Diagnostika, 67(3), 151-157.
Camejo, M., Segnini, A., & Proverbio, F. (2001). Interleukin-6 (IL-6) in seminal plasma of infertile men, and lipid peroxidation of their sperm. Archives of andrology, 47(2), 97-101.
Cao, W., Wang, X., Chen, T., Zhu, H., Xu, W., Zhao, S., Cheng, X., & Xia, L. (2015). The expression of notch/notch ligand, IL-35, IL-17, and Th17/Treg in preeclampsia. Disease markers, 2015.
Collison, L. W., Chaturvedi, V., Henderson, A. L., Giacomin, P. R., Guy, C., Bankoti, J., Finkelstein, D., Forbes, K., Workman, C. J., & Brown, S. A. (2010). IL-35-mediated induction of a potent regulatory T cell population. Nature immunology, 11(12), 1093-1101.
Dehghan Marvast, L., Aflatoonian, A., Talebi, A., Ghasemzadeh, J., & Pacey, A. (2016). Semen inflammatory markers and Chlamydia trachomatis infection in male partners of infertile couples. Andrologia, 48(7), 729-736.
Djourabchi Borojerdi, A. S., Welchowski, T., Peng, W., Buchen, A., Novak, N., Haidl, G., Duan, Y. G., & Allam, J. P. (2020). Human spermatozoa of male patients with subfertility express the interleukin‐6 receptor. Andrologia, 52(4), e13511.
Emokpae, M. A., & Moronkeji, M. A. (2020). Association of copper-to zinc ratio with sperm concentration among males investigated for infertility. J Infertil Reprod Biol, 8(3), 49-52.
Ferlin, A., & Foresta, C. (2020). Infertility: Practical Clinical Issues for Routine Investigation of the Male Partner. Journal of Clinical Medicine, 9(6), 1644.
Ganaiem, M., AbuElhija, M., Lunenfeld, E., Cherniy, N., Weisze, N., Itach, S. B.-S., Breitbart, H., Apte, R., & Huleihel, M. (2009). Effect of interleukin-1 receptor antagonist gene deletion on male mouse fertility. Endocrinology, 150(1), 295-303.
Gong, J., Zeng, Q., Yu, D., & Duan, Y.-G. (2020). T lymphocytes and testicular immunity: a new insight into immune regulation in testes. International Journal of Molecular Sciences, 22(1), 57.
Gruschwitz, M. S., Brezinschek, R., & Brezinschek, H. P. (1996). Cytokine levels in the seminal plasma of infertile males. Journal of Andrology, 17(2), 158-163.
Havrylyuk, A., Chopyak, V., Boyko, Y., Kril, I., & Kurpisz, M. (2015). Cytokines in the blood and semen of infertile patients. Central-European journal of immunology, 40(3), 337.
Karavolos, S., Panagiotopoulou, N., Alahwany, H., & Martins da Silva, S. (2020). An update on the management of male infertility. The Obstetrician & Gynaecologist, 22(4), 267-274.
Keel, B. A. (2004). How reliable are results from the semen analysis? Fertility and sterility, 82(1), 41-44.
Ko, E. Y., Sabanegh Jr, E. S., & Agarwal, A. (2014). Male infertility testing: reactive oxygen species and antioxidant capacity. Fertility and sterility, 102(6), 1518-1527.
Kumar, N., & Singh, A. K. (2022, 2022/01/10). Impact of environmental factors on human semen quality and male fertility: a narrative review. Environmental Sciences Europe, 34(1), 6. https://doi.org/10.1186/s12302-021-00585-w
Lyons, H. E., Arman, B. M., Robertson, S. A., & Sharkey, D. J. (2023). Immune regulatory cytokines in seminal plasma of healthy men: A scoping review and analysis of variance. Andrology.
Organization, W. H. (2021). WHO laboratory manual for the examination and processing of human semen. World Health Organization.
Oyetunji, O. A., Samson, A. O., Adekemi, A. T., & Feyisike, J. O. (2021). Attenuating effect of polyalthia longifolia on cadmium sulfate-induced testicular toxicity. Journal of Infertility and Reproductive Biology, 9(2), 52-58.
Rahbar, A., & Abbasi, M. (2020). A Brief Clinical Overview of Etiological Factors in Infertility. Journal of Infertility and Reproductive Biology, 8, 6-8.
Robertson, S. A., & Sharkey, D. J. (2016). Seminal fluid and fertility in women. Fertility and sterility, 106(3), 511-519.
Rong, C., Weng, L., Li, M., Zhou, L., & Li, Y. (2023). Serum interleukin-38 and-41 levels as candidate biomarkers in male infertility. Immunology Letters, 255, 47-53.
Salas-Huetos, A., & Aston, K. I. Defining new genetic etiologies of male infertility: progress and future prospects.
Seshadri, S., Bates, M., Vince, G., & Jones, D. L. (2009). The role of cytokine expression in different subgroups of subfertile men. American Journal of Reproductive Immunology, 62(5), 275-282.
Sharkey, D. J., Tremellen, K. P., Briggs, N. E., Dekker, G. A., & Robertson, S. A. (2017). Seminal plasma pro-inflammatory cytokines interferon-γ (IFNG) and CXC motif chemokine ligand 8 (CXCL8) fluctuate over time within men. Human Reproduction, 32(7), 1373-1381.
Syriou, V., Papanikolaou, D., Kozyraki, A., & Goulis, D. G. (2018). Cytokines and male infertility. European Cytokine Network, 29(3), 73-82.
Terayama, H., Yoshimoto, T., Hirai, S., Naito, M., Qu, N., Hatayama, N., Hayashi, S., Mitobe, K., Furusawa, J.-i., & Mizoguchi, I. (2014). Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege. PLoS One, 9(4), e96120.
Vignali, D. A., & Kuchroo, V. K. (2012). IL-12 family cytokines: immunological playmakers. Nature immunology, 13(8), 722-728.
Wang, C., & Swerdloff, R. S. (2014). Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertility and sterility, 102(6), 1502-1507.
Yoshida, S., Harada, T., Iwabe, T., Taniguchi, F., Mitsunari, M., Yamauchi, N., Deura, I., Horie, S., & Terakawa, N. (2004). A combination of interleukin-6 and its soluble receptor impairs sperm motility: implications in infertility associated with endometriosis. Human Reproduction, 19(8), 1821-1825.
Yue, C.-y., Zhang, B., & Ying, C.-m. (2015). Elevated serum level of IL-35 associated with the maintenance of maternal-fetal immune tolerance in normal pregnancy. PLoS One, 10(6), e0128219.
Zhang, J., Zhang, Y., Wang, Q., Li, C., Deng, H., Si, C., & Xiong, H. (2019). Interleukin‐35 in immune‐related diseases: protection or destruction. Immunology, 157(1), 13-20.
Zhao, S., Zhu, W., Xue, S., & Han, D. (2014, Sep). Testicular defense systems: immune privilege and innate immunity. Cell Mol Immunol, 11(5), 428-437. https://doi.org/10.1038/cmi.2014.38
Zhou, C., Zhang, J., Chen, Y., Wang, H., & Hou, J. (2017). Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. OncoTargets and therapy, 10, 3485.
Experimental animal Biology
Volume 12, Issue 1 - Serial Number 45
No.1
September 2023
Pages 45-55
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