Identification of gene locus polymorphism involved in heat stress ‎‎(HSP90β) in Marandi indigenous and commercial chickens‎

Pish Jang Aghajeri, Jafar; Najafi, Ghader; Pish Jang Aghajari, Parsa; Mohammad Goli Famiyan, Fatemeh; Mehri, Elnaz

doi:10.30473/eab.2023.65118.1884

Document Type : Article

Authors

¹ Department of Animal Science, ‎Maragheh Branch, Islamic Azad ‎University, Maragheh, Iran

² ‎Department of Pathobiology, Faculty ‎of Veterinary, Urmia Branch, Islamic ‎Azad University, Urmia, Iran

³ ‎Department of Basic Sciences, ‎Bonab Branch, Islamic Azad ‎University, Bonab, Iran

⁴ ‎Department of Basic Sciences, ‎Maragheh Branch, Islamic Azad ‎University, Maragheh, Iran

10.30473/eab.2023.65118.1884

Abstract

Heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gene involved in heat stress (HSP90β) in Marandi indigenous, broiler and laying chickens using PCR-RFLP technique. Randomly, blood was taken from 300 chicken and genomic DNA was extracted by salting out method. Amplification of the desired gene locus with a length of 494 bp was performed using specific primers and MspI enzyme was used to identify the mutation in the desired gene locus. After enzymatic digestion, two genotypes M¹M¹ and M¹M² and two alleles M¹ (with a band of 494 bp) and M² (with two bands of 248 bp and 246 bp) were identified for the HSP90β marker locus. Marandi indigenous and broiler chicken masses were in the Hardy-Weinberg equilibrium. For Marandi indigenous and broiler chicken populations, the Shannon information index at the HSP90β marker locus was 0.25 and 0.40, the fixation index is -0.07 and -0.16, and the observed heterozygosity index was 0.14 and 0.28, respectively. Due to the presence of polymorphism and mutation in the studied gene locus, it can be used in the Marandi indigenous and broiler chickens by genetic selection with the help of this marker to eliminate heat-sensitive chickens and keep heat-resistant chickens.

Keywords

Main Subjects

Veterinary

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Experimental animal Biology

Identification of gene locus polymorphism involved in heat stress ‎‎(HSP90β) in Marandi indigenous and commercial chickens‎

References

References

Volume 12, Issue 1 - Serial Number 45
No.1
September 2023
Pages 1-8

Identification of gene locus polymorphism involved in heat stress ‎‎(HSP90β) in Marandi indigenous and commercial chickens‎

References

References

Volume 12, Issue 1 - Serial Number 45 No.1September 2023Pages 1-8

Volume 12, Issue 1 - Serial Number 45
No.1
September 2023
Pages 1-8