Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Comparision of J. regia Shell Extract and Glibenclamide on Biochemical Blood Parameters in Alloxan-Induced Diabetic Rats Comparision of J. regia Shell Extract and Glibenclamide on Biochemical Blood Parameters in Alloxan-Induced Diabetic Rats 1 8 1311 FA Roya Mir-Badalzadeh Zahra Shirdel Journal Article 2013 01 16 Abstract Juglon is the most available flavonoide in walnut green skin and its antidiabetic effects has attracted researchers in the recent years. The aim of this study is to investigation of J.regia husk hydroalcoholic extract on biochemical blood parameters in diabetic rats. In present study, 40 male rats divided in 4 groups. 1 (normal saline) was treated by saline. 2 (control) was treated by alloxan monohydrate. 3 (extract) included diabetic rats that received hydro-alcoholic extract of J.regia. 4 (glibenclamide) included diabetic rats that received glibenclamide. The results showed significant reduction of glucose– triglyceride and LDL levels in group 3 in comparison with group 2 (P<0.001). The extract also could increase HDL levels significantly compared with group 2 (P<0.001). According to the results of the present study, the extract of walnut green skin has a certain antidiabetic effect and reduces the blood glucose, lipoproteins TG, LDL, and increases the blood HDL. However to determine the exact mechanism needs to be broader studies. Keywords: J. regia hydroalcoholic extract, Diabetes mellitus, Glibenclamide, Rat. Abstract Juglon is the most available flavonoide in walnut green skin and its antidiabetic effects has attracted researchers in the recent years. The aim of this study is to investigation of J.regia husk hydroalcoholic extract on biochemical blood parameters in diabetic rats. In present study, 40 male rats divided in 4 groups. 1 (normal saline) was treated by saline. 2 (control) was treated by alloxan monohydrate. 3 (extract) included diabetic rats that received hydro-alcoholic extract of J.regia. 4 (glibenclamide) included diabetic rats that received glibenclamide. The results showed significant reduction of glucose– triglyceride and LDL levels in group 3 in comparison with group 2 (P<0.001). The extract also could increase HDL levels significantly compared with group 2 (P<0.001). According to the results of the present study, the extract of walnut green skin has a certain antidiabetic effect and reduces the blood glucose, lipoproteins TG, LDL, and increases the blood HDL. However to determine the exact mechanism needs to be broader studies. Keywords: J. regia hydroalcoholic extract, Diabetes mellitus, Glibenclamide, Rat. https://eab.journals.pnu.ac.ir/article_1311_da539cbd9d83e85781c59d077e5600ff.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Comparison of Antibacterial Effect of ZnO Nanoparticles in Apple Juice at 25 and 4° C Comparison of Antibacterial Effect of ZnO Nanoparticles in Apple Juice at 25 and 4° C 9 15 1312 FA Mahboobeh MirHoseeni Molod Kyani Harchghani Sherin Kakai Dehkordi Fatemeh Barzghari Firozabadi Journal Article 2013 01 16 Abstract Nanoparticles (NPs) are increasingly recognized as their usage in biological applications including nanomedicine and food safety. Purpose of this study was Study on Comparison of antibacterial effect of ZnO nanoparticles in apple juice at 25 and 4 ° C. The present study investigated the antibacterial activity of ZnO was tested against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Bacillus cereus in apple juice during storage at 25◦C and 4◦C. The bactericidal activity of the ZnO depended upon storage temperature, ZnO concentration, and microorganism type. ZnO was more effective at 25C ◦ than at 4C ◦ in studied apple juice. Bacillus cereus was more resistant to ZnO than Listeria monocytogenes, Escherichia coli, and Staphylococcus aureus.This study suggested that the application of ZnO NPs as antibacterial agents in food systems and medicine may be effective at inhibiting certain pathogens. Keywords: Foods, Nanoparticles, Pathogens, Antibacterial agents, Zinc oxide. Abstract Nanoparticles (NPs) are increasingly recognized as their usage in biological applications including nanomedicine and food safety. Purpose of this study was Study on Comparison of antibacterial effect of ZnO nanoparticles in apple juice at 25 and 4 ° C. The present study investigated the antibacterial activity of ZnO was tested against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Bacillus cereus in apple juice during storage at 25◦C and 4◦C. The bactericidal activity of the ZnO depended upon storage temperature, ZnO concentration, and microorganism type. ZnO was more effective at 25C ◦ than at 4C ◦ in studied apple juice. Bacillus cereus was more resistant to ZnO than Listeria monocytogenes, Escherichia coli, and Staphylococcus aureus.This study suggested that the application of ZnO NPs as antibacterial agents in food systems and medicine may be effective at inhibiting certain pathogens. Keywords: Foods, Nanoparticles, Pathogens, Antibacterial agents, Zinc oxide. https://eab.journals.pnu.ac.ir/article_1312_0cd0da991224d6a164a1674819aaf79c.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Identification of Plant Parasitic Nematodes of Lucerne Fields in Ahar-EastAzarbaijan Province and Introduction Three New Species of Belonolaimidae Family of Iran Identification of Plant Parasitic Nematodes of Lucerne Fields in Ahar-EastAzarbaijan Province and Introduction Three New Species of Belonolaimidae Family of Iran 17 23 1313 FA Shafighe Eskandary Gholamreza Niknam Akbar Karghar Bideh Journal Article 2013 01 16 Abstract In order to identify biodiversity of plant parasitic nematodes in alfalfa and no comprehensive research about the plant parasitic nematodes associated with alfalfa,during 2002-2003, 33 soil samples were collected from alfalfa fields of several locations in alfalfa fields in Ahar region, partly biodiversity plant parasitic nematodes in this crop were identified.Nematodes extracted by combined sieving and centrifugal-floatation Jenkinse method and transferred to glycerine by De Grisse,s method. After preparation pemanent microscopically slides examined under the light microscope. Measurements and drawing of nematodes were carried out using by drawing tube equipped the microscope. Different species were identified on the basis of morphological characters as well as morphometric parameters and available literature. In this survey 20 species of 11 genera belonging sub order tylenchina included: Boleodorus thylactus, Coslenchus aquaticus, Criconemella antipolitana, Ditylenchus dipsaci, Ditylenchus medicaginis, Geocenamus brevidens, Geocenamus nanus, Geocenamus quadrifer, Helicotylenchus digonicus, Helicotylenchus pseudorobustus, Helicotylenchus vulgaris, Paratylenchus italiensis, Paratylenchus tateae, Pratylenchus scribneri, Pratylenchus neglectus, Pratylenchus thornei, Psilenchus iranicus, Tylenchoryhnchus parvus, Tylenchoryhnchus striatus, Zygotylenchus guevarai. Among the recognized genera and species, Geocenamus nanus, Tylenchoryhnchus striatus and Tylenchoryhnchus parvus are reported for the first time from lucerne fields of Iran. Abstract In order to identify biodiversity of plant parasitic nematodes in alfalfa and no comprehensive research about the plant parasitic nematodes associated with alfalfa,during 2002-2003, 33 soil samples were collected from alfalfa fields of several locations in alfalfa fields in Ahar region, partly biodiversity plant parasitic nematodes in this crop were identified.Nematodes extracted by combined sieving and centrifugal-floatation Jenkinse method and transferred to glycerine by De Grisse,s method. After preparation pemanent microscopically slides examined under the light microscope. Measurements and drawing of nematodes were carried out using by drawing tube equipped the microscope. Different species were identified on the basis of morphological characters as well as morphometric parameters and available literature. In this survey 20 species of 11 genera belonging sub order tylenchina included: Boleodorus thylactus, Coslenchus aquaticus, Criconemella antipolitana, Ditylenchus dipsaci, Ditylenchus medicaginis, Geocenamus brevidens, Geocenamus nanus, Geocenamus quadrifer, Helicotylenchus digonicus, Helicotylenchus pseudorobustus, Helicotylenchus vulgaris, Paratylenchus italiensis, Paratylenchus tateae, Pratylenchus scribneri, Pratylenchus neglectus, Pratylenchus thornei, Psilenchus iranicus, Tylenchoryhnchus parvus, Tylenchoryhnchus striatus, Zygotylenchus guevarai. Among the recognized genera and species, Geocenamus nanus, Tylenchoryhnchus striatus and Tylenchoryhnchus parvus are reported for the first time from lucerne fields of Iran. https://eab.journals.pnu.ac.ir/article_1313_8dbcd2c8ac01cd3354156a80faab4bc8.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Investigation of Antibiotic Susceptibility Pattern of E.coli Isolated from Urinary Culture Related to Patients in the West of the Mazandaran Province, during from September 2010 to September 2011 Investigation of Antibiotic Susceptibility Pattern of E.coli Isolated from Urinary Culture Related to Patients in the West of the Mazandaran Province, during from September 2010 to September 2011 25 32 1321 FA AliMohamad Asgharyan Journal Article 2013 01 17 Abstract E.coli is known one of the most important bacteria as it causes some problems such as urinary tract and noncosomial. In this study, the antimicrobial susceptibility of E.coli in the urine culture in the west of Mazandaran province was determined. For this descriptive study, a total of 357 urine samples were collected over a 12 month period. Then all specimens included in this study were routinely cultured on the EMB and MacConkey plates. All suspected colonies were identified and confirmed by Gram staining, colony characteristics, motility and biochemical reactions by MIVP tests for each isolates. Susceptibility tests were done by disc diffusion method according NCCLS standards. Out of 357 specimens, 302 samples were positive cultures for E.coli. According to our results of antimicrobial tests, the highest susceptibilities were to Ciprofloxacin, Gentamicin, Nitrofurantoin with 48.4%, 43.3% and 37.9%, respectively. Also statistical analysis by SPSS, chi square test, showed that sensitivity to some antibiotics such as Ceftriaxon, Nalidixic acid and Ceftizoxime was significant (χ2 test, P≤5) between men and women. In our study, the highest sensitivity for E.coli isolates was to ciprofloxacin and the rate of E.coli related sensitivity for most of antibiotics was not considerable. Abstract E.coli is known one of the most important bacteria as it causes some problems such as urinary tract and noncosomial. In this study, the antimicrobial susceptibility of E.coli in the urine culture in the west of Mazandaran province was determined. For this descriptive study, a total of 357 urine samples were collected over a 12 month period. Then all specimens included in this study were routinely cultured on the EMB and MacConkey plates. All suspected colonies were identified and confirmed by Gram staining, colony characteristics, motility and biochemical reactions by MIVP tests for each isolates. Susceptibility tests were done by disc diffusion method according NCCLS standards. Out of 357 specimens, 302 samples were positive cultures for E.coli. According to our results of antimicrobial tests, the highest susceptibilities were to Ciprofloxacin, Gentamicin, Nitrofurantoin with 48.4%, 43.3% and 37.9%, respectively. Also statistical analysis by SPSS, chi square test, showed that sensitivity to some antibiotics such as Ceftriaxon, Nalidixic acid and Ceftizoxime was significant (χ2 test, P≤5) between men and women. In our study, the highest sensitivity for E.coli isolates was to ciprofloxacin and the rate of E.coli related sensitivity for most of antibiotics was not considerable. https://eab.journals.pnu.ac.ir/article_1321_363d94f2d4f3bf70a6615372384403bd.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Improvement of Performance in Six Silkworm F1 Hybrids by means of Nine Selection Generations using Index System Improvement of Performance in Six Silkworm F1 Hybrids by means of Nine Selection Generations using Index System 33 40 1322 FA Pedram Poursmaeli S.Z Mirhossini Mani Ghanipoor Abdolahd Shadparvar Alireza Seidav Journal Article 2013 01 17 Abstract Effect of selection index in four Iranian commercial lines (107, 110, 153 and 154) on performance of their hybrids including 107‌×110, 110×107, 153×154 and 154×153 was investigated. Selection index program was performed on all 3P commercial lines during 9 generations. Next generation parents of 3P lines were selected in each generation, by construction of selection matrix based on heritability parameters, correlations between cocoon weight, cocoon shell weight and cocoon shell percentage and also phenotypic mean of traits. In the 10th generation, 2P populations (produced by random cross of 3P) were mated randomly and P (parent) silkworm eggs were produced by random mating of 2P population. P populations in selected and random groups were reared under rural (silkworm rearing house) conditions. Control P population was produced by crossing 2P populations (produced by non-selected 3P lines). F1 hybrids of control group (non-selected) were reared simultaneously. From obtained results, it was showed selection by index method in 3P lines has significant effect on performance improvement of silkworm F1 hybrids. There was significant difference for cocoon weight between two selected (1.556gr) and non-selected (1.486gr) groups (P<0.05). Meanwhile shell cocoon weight had significant difference between two selected (0.344gr) and non-selected (0.310gr) groups (P<0.05). Also, cocoon shell percentage showed significant difference between two selected (22.268%) and non-selected index (21.037%) groups (P<0.05). Abstract Effect of selection index in four Iranian commercial lines (107, 110, 153 and 154) on performance of their hybrids including 107‌×110, 110×107, 153×154 and 154×153 was investigated. Selection index program was performed on all 3P commercial lines during 9 generations. Next generation parents of 3P lines were selected in each generation, by construction of selection matrix based on heritability parameters, correlations between cocoon weight, cocoon shell weight and cocoon shell percentage and also phenotypic mean of traits. In the 10th generation, 2P populations (produced by random cross of 3P) were mated randomly and P (parent) silkworm eggs were produced by random mating of 2P population. P populations in selected and random groups were reared under rural (silkworm rearing house) conditions. Control P population was produced by crossing 2P populations (produced by non-selected 3P lines). F1 hybrids of control group (non-selected) were reared simultaneously. From obtained results, it was showed selection by index method in 3P lines has significant effect on performance improvement of silkworm F1 hybrids. There was significant difference for cocoon weight between two selected (1.556gr) and non-selected (1.486gr) groups (P<0.05). Meanwhile shell cocoon weight had significant difference between two selected (0.344gr) and non-selected (0.310gr) groups (P<0.05). Also, cocoon shell percentage showed significant difference between two selected (22.268%) and non-selected index (21.037%) groups (P<0.05). https://eab.journals.pnu.ac.ir/article_1322_15f08d495a493ceab93fa42b5d339340.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Identification, Determination of Abundance and Distribution of Plant Parasitic Nematodes in Orchards and Fields in Qom Province Identification, Determination of Abundance and Distribution of Plant Parasitic Nematodes in Orchards and Fields in Qom Province 41 49 1323 FA Zolfaghar Lotfi Azadeh Gharakhani Journal Article 2013 01 17 Abstract In order to identify plant parasitic nematodes associated with orchards in Qom Province and determining their distribution, 80samples of soil and root were collected from different locations, during spring and autumn of 2011 the samples were washed and nematodes were extracted by centrifugal floatation technique. They were fixed and transfered to glycerin according to the De Grissemethod. The permanent slides were prepared from the extracted nematodes and some cross sections were made from different parts of the body.After preparation of slides, morphological and morphometrical characters specimens were studied by light microscope attached to drawing tube and identification of speciesis determinated with purvey perfect description and comparision with initial description and other valid science refrences. In result 28 species belonging to different genera of Tylenchida and one species of Dorylaimida and were identified and their distribution were determined. Results of this survey showed that the most of these and four species belong to the genera of Ditylenchus and Aphelenchoides respectively that there are in most of orchards. Macropostoniaxenoplax, Scutylenchusrugosus, Pratylenchusneglectus and Xiphinemaindexwere found in 62.5, 58.5, 37.5 and 37.5 percent of samples, respectively, and they were the most aboundant species in the fruit trees of the province. Abstract In order to identify plant parasitic nematodes associated with orchards in Qom Province and determining their distribution, 80samples of soil and root were collected from different locations, during spring and autumn of 2011 the samples were washed and nematodes were extracted by centrifugal floatation technique. They were fixed and transfered to glycerin according to the De Grissemethod. The permanent slides were prepared from the extracted nematodes and some cross sections were made from different parts of the body.After preparation of slides, morphological and morphometrical characters specimens were studied by light microscope attached to drawing tube and identification of speciesis determinated with purvey perfect description and comparision with initial description and other valid science refrences. In result 28 species belonging to different genera of Tylenchida and one species of Dorylaimida and were identified and their distribution were determined. Results of this survey showed that the most of these and four species belong to the genera of Ditylenchus and Aphelenchoides respectively that there are in most of orchards. Macropostoniaxenoplax, Scutylenchusrugosus, Pratylenchusneglectus and Xiphinemaindexwere found in 62.5, 58.5, 37.5 and 37.5 percent of samples, respectively, and they were the most aboundant species in the fruit trees of the province. https://eab.journals.pnu.ac.ir/article_1323_6d5fb608a8ae73e3781afe7820f620cc.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Identification Snails of Dez River in Khuzestan Province and Introduction Two New Records from Iran Identification Snails of Dez River in Khuzestan Province and Introduction Two New Records from Iran 51 62 1324 FA Zahra Ektefa Elham Ahmadi Freshteh Shah Mohamadi Journal Article 2013 01 17 Abstract Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters including shape, size of shell, dextral and sinistral position of the shell, operculum, aperture, radula, mucus colour, respiratory pore, keel, renal ridge and reproduction system. The most and least distribution among identified snail species are 18 and 8 species belong to 4 and 2 stations respectively from Dez river, which they are 20/82 kilometers away from each other. The most commonly distributed family is Thiaridae with 8 species Melanopsispraemorsa, Melanopsis costata, Melanopsis doriae Melanopsis nodosa, Melanopsis subtingitana, Melanoides pyramis, Melanoides tuberculata Thiara scabra. Bulinus truncates and Lymnaea truncatula are identified as the most frequent and has medical importance. Melanopsis subtingitana Annandale, 1918 and Gyraulus huwaizahensis species are reported for the first time from Iran. Abstract Collection and identification snails of Dez river in Khuzestan province in Iran has been investigated in 2012-2013. For the first time, 29 snail species are identified in Dez river in Khuzestan, which they belong to 15 genera from 10 families Identification was done according to morphometric characters including shape, size of shell, dextral and sinistral position of the shell, operculum, aperture, radula, mucus colour, respiratory pore, keel, renal ridge and reproduction system. The most and least distribution among identified snail species are 18 and 8 species belong to 4 and 2 stations respectively from Dez river, which they are 20/82 kilometers away from each other. The most commonly distributed family is Thiaridae with 8 species Melanopsispraemorsa, Melanopsis costata, Melanopsis doriae Melanopsis nodosa, Melanopsis subtingitana, Melanoides pyramis, Melanoides tuberculata Thiara scabra. Bulinus truncates and Lymnaea truncatula are identified as the most frequent and has medical importance. Melanopsis subtingitana Annandale, 1918 and Gyraulus huwaizahensis species are reported for the first time from Iran. https://eab.journals.pnu.ac.ir/article_1324_8f581a6a41a3148b617706ad86c66cf6.pdf

Payame Noor University Experimental animal Biology 2322-2387 2 3 2013 12 22 Investigation of Carapace Frequency, Length-Weight Relationship and Biomass of Jinga Shrimp (Metapenaeus affinis) in Shrimp Fishing Grounds of Hormozgan Province Investigation of Carapace Frequency, Length-Weight Relationship and Biomass of Jinga Shrimp (Metapenaeus affinis) in Shrimp Fishing Grounds of Hormozgan Province 63 74 1325 FA Mohamadhasan Gerami Seed Yosef Peghambari Rasoul Ghorbani Journal Article 2013 01 17 Abstract This study, aimed to achieve carapace length frequency, depth distribution, determining biomass and length-weight relationship of Metapenaeus affinis in shrimp fishing grounds of Hormomzgan province. Sampling was carried out by Trawl net and swept area method from January 2010 to February 2011. Samples were transferred to the laboratory and biometric information entered on special forms. Maximum Carapace length was 35 and 47 mm for males and females, respectively. Maximum Carapace length frequency was belonged to 20-22 mm range for both sexes. Also maximum carapace length of population was found in March for both sexes. Length-weight relationship was W = 3E-06TL3.1465 for males and W = 4E-06TL3.149 for females. According to the b factor, Metapenaeus affinis had a positive allometric growth. Biomass of M. affinis was calculated 822 ton in 2011. In the distribution of deep layers, it was found that maximum biomass was related to layer 10-20. Abstract This study, aimed to achieve carapace length frequency, depth distribution, determining biomass and length-weight relationship of Metapenaeus affinis in shrimp fishing grounds of Hormomzgan province. Sampling was carried out by Trawl net and swept area method from January 2010 to February 2011. Samples were transferred to the laboratory and biometric information entered on special forms. Maximum Carapace length was 35 and 47 mm for males and females, respectively. Maximum Carapace length frequency was belonged to 20-22 mm range for both sexes. Also maximum carapace length of population was found in March for both sexes. Length-weight relationship was W = 3E-06TL3.1465 for males and W = 4E-06TL3.149 for females. According to the b factor, Metapenaeus affinis had a positive allometric growth. Biomass of M. affinis was calculated 822 ton in 2011. In the distribution of deep layers, it was found that maximum biomass was related to layer 10-20. https://eab.journals.pnu.ac.ir/article_1325_91e41b7e51ab8a49ace72f9387a0ff27.pdf