Payame Noor University Experimental animal Biology 2322-2387 11 1 2022 06 22 Evaluation of the Effect of DCA-‎Treated Mesenchymal Stem Cell ‎Conditioned Media on Biological ‎Behaviors of Breast Cancer Evaluation of the Effect of DCA-‎Treated Mesenchymal Stem Cell ‎Conditioned Media on Biological ‎Behaviors of Breast Cancer 65 75 9429 10.30473/eab.2023.62849.1868 FA Narges Toolabi M.A., Department of Animal Sciences and Marine Biology, ‎Faculty of Life Sciences and Biotechnology, Shahid Beheshti ‎University, Tehran, Iran Mahmood Talkhabi Assistant Professor, Department of Animal Sciences and Marine ‎Biology, Faculty of Life Sciences and Biotechnology, Shahid ‎Beheshti University, Tehran, Iran‎ Fattane Sam Daliri M.A., Department of Animal Sciences and Marine Biology, ‎Faculty of Life Sciences and Biotechnology, Shahid Beheshti ‎University, Tehran, Iran Farnoosh Attari Assistant Professor, Department of Animal Biology, School of ‎Biology, College of Science, University of Tehran, Tehran, Iran‎ Leila Taghiyar Assistant Professor, Department of Stem Cells and ‎Developmental Biology, Cell Science Research Center, Royan ‎Institute for Stem Cell Biology and Technology, ACECR, Tehran, ‎Iran Journal Article 2022 02 03 Cancer is the result of the overgrowth of malignant cells that have the ability to spread to other parts of the body. Dichloroacetate (DCA) has been considered as a new drug to control various cancers. The effects of stem cells or their conditioned media (CM) on the treatment or control of some cancers have also been shown. In this study, human adipose-derived mesenchymal stem cells (h-ADMSCs) and breast cancer cell line 4T1 were first treatedwith different concentrations of DCA and their viability was assessed by MTT assay and 1mM were selected for CM collection. h-ADMSCs with four groups including groups with no FBS media, ± DCA (-FBS /±1mM DCA) and groups with 5% FBS ± DCA (+5%FBS/±1mMDCA) were treated to prepare CM. Then the viability, colony forming potential, cell cycle profile and apoptosis of CM-treated 4T1 cells were investigated. The results showed that CM in the -FBS/+DCA group decreased the viability (P-Value <0.05) and increased the proliferation of 4T1 cells, compared to the -FBS/-DCA group, respectively. Compared to +5%FBS/-DCA group, CM of +5% FBS/+DCA were able to increase viability and proliferation of 4T1 cells. Also, CM of the four studied groups caused changes in the rate of apoptosis and cell cycle profile of 4T1 cells. It seems that, DCA can increase the viability and proliferation of 4T1 breast cancer cells by affecting on thecomposition of mesenchymal stem cells CM. Cancer is the result of the overgrowth of malignant cells that have the ability to spread to other parts of the body. Dichloroacetate (DCA) has been considered as a new drug to control various cancers. The effects of stem cells or their conditioned media (CM) on the treatment or control of some cancers have also been shown. In this study, human adipose-derived mesenchymal stem cells (h-ADMSCs) and breast cancer cell line 4T1 were first treatedwith different concentrations of DCA and their viability was assessed by MTT assay and 1mM were selected for CM collection. h-ADMSCs with four groups including groups with no FBS media, ± DCA (-FBS /±1mM DCA) and groups with 5% FBS ± DCA (+5%FBS/±1mMDCA) were treated to prepare CM. Then the viability, colony forming potential, cell cycle profile and apoptosis of CM-treated 4T1 cells were investigated. The results showed that CM in the -FBS/+DCA group decreased the viability (P-Value <0.05) and increased the proliferation of 4T1 cells, compared to the -FBS/-DCA group, respectively. Compared to +5%FBS/-DCA group, CM of +5% FBS/+DCA were able to increase viability and proliferation of 4T1 cells. Also, CM of the four studied groups caused changes in the rate of apoptosis and cell cycle profile of 4T1 cells. It seems that, DCA can increase the viability and proliferation of 4T1 breast cancer cells by affecting on thecomposition of mesenchymal stem cells CM. https://eab.journals.pnu.ac.ir/article_9429_f36e077daa9fc70ca1f5794268db04d4.pdf