Payame Noor University Experimental animal Biology 2322-2387 7 4 2019 04 21 Investigation of the Toxic Effects of Copper Oxide Nanoparticles on the Structure of Gonadal Tissue in Male Investigation of the Toxic Effects of Copper Oxide Nanoparticles on the Structure of Gonadal Tissue in Male 117 124 6399 10.30473/eab.2019.6399 FA Sajjad Rajabi M.A. in Animal Physiology, Payam Noor University ‎of Tehran, Iran , Fatemeh Shahbazi Assistant Professor, Department of Biology, Payame ‎Noor University, Iran ‎ Ali Noori Professor, Department of Biology, Falavarjan Branch, ‎Islamic Azad University, Isfahan, Iran Raziye Karshenas M.A. in Animal Physiology, Payam Noor University ‎of Tehran, Iran Journal Article 2020 01 08 <strong>Abstract</strong> <br />Since copper nanoparticles are one of the first nanoparticles planned in the industry, recent research has shown the pathological toxicity of these nanoparticles in different tissues and organs. Therefore, the purpose of this study was to investigate the effect of copper oxide nanoparticles on gonadal tissues in male rats. In this study, 40 Wistar rats were divided in four groups control group and 10, 20, 30 (mg/ kg) of copper oxide nanoparticles, respectively 5 Times, received for one day in a row for 10 days. In order to histopathologic studies, the testicular tissue samples were stained with hematoxylin-eosin. The data were analyzed in the spss software, one –way anova and duncan's test. The results showed that the amount of spermatogonial cells, primary spermatocyte, spermatid cells were decreased in dose-dependent manner. In the testicle, disorders such as abnormality and severe deformity with different morphology in the spermicidal tubes and destruction of sertoli cells were observed. The results of the studies showed that copper oxide nanoparticles with oxidative stress and cellular degradation disrupted the structure and process of gonadal spermatogenesis which due to the absence of mortality in mice may eventually overcome disturbances in normal conditions. <strong>Abstract</strong> <br />Since copper nanoparticles are one of the first nanoparticles planned in the industry, recent research has shown the pathological toxicity of these nanoparticles in different tissues and organs. Therefore, the purpose of this study was to investigate the effect of copper oxide nanoparticles on gonadal tissues in male rats. In this study, 40 Wistar rats were divided in four groups control group and 10, 20, 30 (mg/ kg) of copper oxide nanoparticles, respectively 5 Times, received for one day in a row for 10 days. In order to histopathologic studies, the testicular tissue samples were stained with hematoxylin-eosin. The data were analyzed in the spss software, one –way anova and duncan's test. The results showed that the amount of spermatogonial cells, primary spermatocyte, spermatid cells were decreased in dose-dependent manner. In the testicle, disorders such as abnormality and severe deformity with different morphology in the spermicidal tubes and destruction of sertoli cells were observed. The results of the studies showed that copper oxide nanoparticles with oxidative stress and cellular degradation disrupted the structure and process of gonadal spermatogenesis which due to the absence of mortality in mice may eventually overcome disturbances in normal conditions. https://eab.journals.pnu.ac.ir/article_6399_13ae304054b95eb90e991444b98a2207.pdf