Payame Noor University Experimental animal Biology 2322-2387 8 1 2019 08 23 Effect of Di 2-Ethyl Hexyl phthalate on some indeces of Skin Mucus in common Carp (Cyprinus carpio) Effect of Di 2-Ethyl Hexyl phthalate on some indeces of Skin Mucus in common Carp (Cyprinus carpio) 77 88 5983 10.30473/eab.2019.5983 FA Morteza Kamali M. A. of Biochemistry, Islamic Azad University, Babol Branch, Iran Saeid Alinejad Moaelem Assistant Professor, Department of Biology, Faculty of Natural Resources and Marin Science, Tarbiat Modares University, Tehran, Iran Nastaran Pour-Ahmadi Instructor, Department of Fisheries Science and Engineering, Faculty of Natural Resources and Marine Sciences, Tarbiat Modarres University Journal Article 2019 08 27 <strong>Abstract</strong><strong></strong> <br />Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and the aquatic and human populations. In present study; effects of toxicity of DEHP on protein band model and amino acid profile of mucus common carp (<em>Cyprinus carpio</em>) was studied. 108 common carp (17.60±2.22gr) is gathered from warm water fish center and they were allocated into 9 tanks (12 Fish per tank). Fish adapted to new condition for a week.   During this time; they were feed twice a day with same diets. Fishes were exposed at 3 concentration treatments containing 0; 0.1 and 1 mg/L DEHP under laboratory condition (25±0.5°C; pH: 7.4-8) for a period of 96 hours.    We assessed skin mucus protein profiles by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and we were studied amino acid profile by HPLC. The results of the experiments to compare the protein pattern of carp’s mucus (exposed to DEHP with two different concentrations) with the control sample (without exposure to DEHP) showed that there is a significant difference in the bands density between the two protein patterns. These results indicated that concentrations of DEHP beneficially affects Amino Acid profiles of skin mucus in carp and they were categorized in 4 branches. There were Significant differences between exposed and control groups.  Therefore; fish skin mucus can be a biomarker for showing contaminants and toxins. And the high consumption of plastic materials can be a warning to the health of aquatic animals and humans. <br />  <strong>Abstract</strong><strong></strong> <br />Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and the aquatic and human populations. In present study; effects of toxicity of DEHP on protein band model and amino acid profile of mucus common carp (<em>Cyprinus carpio</em>) was studied. 108 common carp (17.60±2.22gr) is gathered from warm water fish center and they were allocated into 9 tanks (12 Fish per tank). Fish adapted to new condition for a week.   During this time; they were feed twice a day with same diets. Fishes were exposed at 3 concentration treatments containing 0; 0.1 and 1 mg/L DEHP under laboratory condition (25±0.5°C; pH: 7.4-8) for a period of 96 hours.    We assessed skin mucus protein profiles by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and we were studied amino acid profile by HPLC. The results of the experiments to compare the protein pattern of carp’s mucus (exposed to DEHP with two different concentrations) with the control sample (without exposure to DEHP) showed that there is a significant difference in the bands density between the two protein patterns. These results indicated that concentrations of DEHP beneficially affects Amino Acid profiles of skin mucus in carp and they were categorized in 4 branches. There were Significant differences between exposed and control groups.  Therefore; fish skin mucus can be a biomarker for showing contaminants and toxins. And the high consumption of plastic materials can be a warning to the health of aquatic animals and humans. <br />  https://eab.journals.pnu.ac.ir/article_5983_e155b009679efaa942401cfe160b7d68.pdf