Payame Noor University Experimental animal Biology 2322-2387 Articles in Press 2013 12 02 Study on stress indices in the acute and subacute response of Carassius auratus to the aquatic pollutants Study on stress indices in the acute and subacute response of Carassius auratus to the aquatic pollutants 561 FA Elham Abdzadeh University of Guilan Fatemeh Nazarhaghighi University of Guilan Journal Article 2012 11 18 To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor,with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and blood plasma were measured inorganic phosphorous. The results showed that the cortisol and glucose levels on day 5, there was a significant difference between control and Butachlor treatments (P 0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor,with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and blood plasma were measured inorganic phosphorous. The results showed that the cortisol and glucose levels on day 5, there was a significant difference between control and Butachlor treatments (P 0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P