Payame Noor University Experimental animal Biology 2322-2387 12 4 2024 04 20 Synergistic effect of selenium and calcium nanoparticles on the ‎half-life of alkaline serine protease enzyme Synergistic effect of selenium and calcium nanoparticles on the ‎half-life of alkaline serine protease enzyme 65 76 11083 10.30473/eab.2024.71266.1951 FA Asieh Emami Nejad Department of Biology, Payam Noor ‎University (PNU), Tehran, ‎Iran‎ Mohammad Fazilati Department of Biology, Payam Noor ‎University (PNU), Tehran, ‎Iran‎ Hossein Salavati Department of Biology, Payam Noor ‎University (PNU), Tehran, ‎Iran‎ Habiballah Nazem Department of Biology, Payam Noor ‎University (PNU), Tehran, ‎Iran‎ Journal Article 2024 05 12 The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine protease enzymes using selenium and calcium nanoparticles was presented. Due to its high biocompatibility, toxicity, and low cost, these nanoparticles have become one of the most prominent non-metallic oxide nanoparticles, which, with their antioxidant and anti-inflammatory properties, can protect against the destruction of enzymes by free radicals. The results showed that these nanoparticles can significantly increase the half-life of these enzymes. On the other hand, the serine protease enzyme immobilized on selenium nanoparticles has unique biochemical characteristics in the presence and absence of calcium. The obtained results indicate that the stabilized enzyme has high activity and stability in a wide range of temperatures and pH compared to pure and impure-free enzyme. The results showed that the enzyme immobilized on selenium nanoparticles increased its activity by more than 99% in the presence of calcium at the temperature of 45°C. On the other hand, the half-life of the free enzyme increased from 95 minutes in the pure form to 115 minutes in the form immobilized on nanoparticles in the presence of calcium. These results indicate that the presence of nanoparticles can prevent the destruction of enzymes by free radicals. The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine protease enzymes using selenium and calcium nanoparticles was presented. Due to its high biocompatibility, toxicity, and low cost, these nanoparticles have become one of the most prominent non-metallic oxide nanoparticles, which, with their antioxidant and anti-inflammatory properties, can protect against the destruction of enzymes by free radicals. The results showed that these nanoparticles can significantly increase the half-life of these enzymes. On the other hand, the serine protease enzyme immobilized on selenium nanoparticles has unique biochemical characteristics in the presence and absence of calcium. The obtained results indicate that the stabilized enzyme has high activity and stability in a wide range of temperatures and pH compared to pure and impure-free enzyme. The results showed that the enzyme immobilized on selenium nanoparticles increased its activity by more than 99% in the presence of calcium at the temperature of 45°C. On the other hand, the half-life of the free enzyme increased from 95 minutes in the pure form to 115 minutes in the form immobilized on nanoparticles in the presence of calcium. These results indicate that the presence of nanoparticles can prevent the destruction of enzymes by free radicals. https://eab.journals.pnu.ac.ir/article_11083_bfb1ed0071433fef395b06cc2c34c32b.pdf