Veterinary
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment ...
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The objective of this study was to investigation effect of different of probiotic BIOSAF supplementation on the effect of different levels of probiotic BIOSAF on growth performance and immune system response of male Ross 308 broiler. Two hundred twenty five chickens were allocated in the three treatment containing 5 pen and 15 birds on each pen. Diets included 0, 1 or 1.5gr of probiotic BIOSAF/kg diet and considered as control, treatment1 and treatment 2, respectively. On day 20 and 35 of age, birds were injected (i.m) with 0.1 mg of 0.5% sheep red blood cells (SRBC). Antibody production against SRBC was measured after 7 days of inoculation. On the day 10 of age, the chicks were vaccinated against Newcastle disease virus (NDV Generally, usingprobiotic BIOSAF per kg diet has efficient effect on growth performance of male Ross chekien particularly, when supplementation is done in starter period. Also, supplementation probiotic BIOSAF has positive effect on immune response to Newcastle and SRBC. However, 1gr/kg supplementation of probiotic BIOSAF is more suitable than 1.5gr/kg diet.
Veterinary
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity ...
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The objectives of this study was to investigate whether consuming of protected glutamine (PG) before parturition in close up period would affect dry matter intake (DMIkg/d), blood urea nitrogen (BUNmg/dl), body condition score (BCS) and biomarkers of oxidative stress, glutathione peroxidase activity (GPX units/ml PCV) and Total Antioxidant Status (TASmmol/L). Thirty six pregnant Holstein dairy cows were assigned into two treatment groups based on their BCS and expected.calving date in t student examination. Treatment groups consisted of 1)glutamine supplementation 100 g/d per cow from 21d before calving until parturition (F), 2) glutamine unsupplementation from 21d before calving until parturition (N). There weren’t any significant differences among treatments in DMI and BCS on d -21,-14 ,-7 and calving day before parturition. There weren’t significant differences in The total antioxidant status (TAS) but plasma glutathione Peroxidase activity (GPX) was significant difference between two group and was greater for (F) group at -7d before calving (57.44 vs 47.94 and p≤0.05 respectively) and -14 (45.87 vs 41.72 and p≤0.04 respectively) before parturition. It seems that supplementation diets with glutamine on the close up period can enhance plasma glutathione Peroxides activity (GPX) and the best level of formaldehyde for protection of glutamine is 1%.
Veterinary
mokhtar fathi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied ...
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A study with 160 one-day age male broilers (Ross 308) was for six week period, conducted to investigate the some blood parameters, and plasma thyroxin (T4) and triiodothyronine (T3) of broiler chickens with cold induced pulmonary hypertension syndrome (PHS). Chicks in 2 groups of 4 replicates were studied in each group20 chicks for each replicate were used. One group set as control (healthy) group and reared under normal temperature, second group set as PHS group and reared under cold temperature. At the end of the experiment (day42), 2 chicks from each replicate were randomly selected bled and slaughtered. The heart was removed and the right ventricle was dissected away. The ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated. The results showed that hematocrit value, mortality due to ascites, RV/TV index and differential white blood cells count were significantly higher in CT treatment chick (P˂0.05). The concentrations of plasma t4 of CT birds were significantly lower than other group birds at day 21 & 42. it is also, The concentrations of plasma t3 of CT birds were significantly higher than other group birds at day 21(exception day 42) (P˂0.05).
Veterinary
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured ...
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To investigate the correlation of serum lipids with spermatogenic activity 10 male goats were distributed into three equal groups included one, two and three years of age and blood samples were collected from the jugular vein. The serum concentrations of total cholesterol and triglyceride were measured by method Enzymatic, serum levels of lipoproteins were determined by method Precipitation and serum levels of testosterone were measured by method Radio Immuno Assay. For histomorphometric study, the 5 µ sections were made and stained with Hematoxyline-Eosin. Data was statistically analyzed by the one way ANOVA and Pearson correlation tests (p≤0.05). Results showed that serum levels of total cholesterol, triglycerids, VLDL-c and LDL-c had not significant differences between different ages. The serum levels of HDL-c, testosterone, as well as the area of spermatic tubules and testicular interstitium, the diameter of seminiferous tubules, the germinal epithelium height, the number and diameters of the leydig cells; additionally, the number of spermatid cells had significant increase from one year group to two years group and also significant decrease from two years animals to three years group. The serum HDL-c values were significantly correlated with the number of leydig cells as well as serum levels of testosterone. Serum triglycerides values were also inversely correlated with germinal epithelium height. It can be concluded that increased serum levels of HDL-c and triglycerides have respectively a benefit and deleterious effects on the spermatogenic activity and histomorphometric structure of the testis in the male goats of Ilam province.
Veterinary
Navab Ghobadi
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and ...
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The purpose of present study is testing influence of injection oxytocin immidiatly after parturition and injection prostaglandin f2α, 20 to 25 days after parturition in dairy cows upon process of uterus contraction distance between delivery till first estrus distance between parturition till first and second Semenation, amount of pregnancy at first semenation and number of semenation for every pregnancy. In This study 30 Holeshtain dairy cow are used in 2 group of 15(treatment and control group).treatment group recivead 10cc Oxytocin immidiatly after parturition and 5cc prostsglandinF2α,20 to 25 days after parturition.the result statistically using chi sequare and t student test were analyzed with spss.The result show a meaning ful difference(p≥0/05)in uterus contraction ,amount of pregnancy at first Semenation and number of Semenation for every pregnancy between treatment and control group.there is a noticable improvement in percent of pregnancy for Treatment group(47/1percent for treatment group and 23/5percent for control group).The result also shows that injecting oxytocin immidiatly after parturition and prostaglandinf2α 20 to25 days later can hare positive increase in amount of future fertility in dairy cow and also it can decrease economical loss in cow herd. This result can show connection of contraction influence of oxytocin and prostaglandinf2α in myometriom layer of uterus to enjection secrection and early contraction of ovary activity.
Veterinary
Parvin Mohseni; Mahboube Bagheri; Narges Gholamian Adimi
Abstract
Vibrio parahaemolyticus is one of the significant food- and waterborne bacteria. In this study, 110 fresh shrimp samples were randomly collected from retail centers in Kerman City during the summer of 2022. In the laboratory, the samples were homogenized and cultured on TCBS agar for 18-24 hours at 37°C. ...
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Vibrio parahaemolyticus is one of the significant food- and waterborne bacteria. In this study, 110 fresh shrimp samples were randomly collected from retail centers in Kerman City during the summer of 2022. In the laboratory, the samples were homogenized and cultured on TCBS agar for 18-24 hours at 37°C. After incubation, green-blue colonies on the TCBS agar were identified as potential Vibrio colonies and were further confirmed using biochemical tests. For genomic extraction of Vibrio parahaemolyticus strains from the shrimp samples, the boiling method was employed. The presence of the virulence genes tdh and trh was examined through PCR to confirm molecular identification and determine virulence factors. According to the present findings, among the 110 shrimp samples cultured on TCBS agar, 72 samples (65.45%) exhibited green or green-blue colonies. Of these, 23.6% were positive for the tox gene, serving as a marker for Vibrio parahaemolyticus. Regarding the virulence genes, the tdh gene was detected in two Vibrio parahaemolyticus isolates (2.7%), while no samples were positive for the trh gene. This study highlights that, although Vibrio parahaemolyticus is widespread in aquatic environments and seafood, most isolates lack the virulence genes tdh and trh.
Veterinary
Taban Khosravi; Hassan Nayebzadeh; Azadeh Rashidimehr; Majid Hataminia
Abstract
Parasitic infections like hydatid cysts, Dicrocelium, and Fasciola are common among humans and livestock and can significantly impact sheep health. For this purpose, this study was conducted to determine the prevalence of hydatid cyst, Dicrocelium dendriticum and Fasciola spp. in slaughtered sheep in ...
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Parasitic infections like hydatid cysts, Dicrocelium, and Fasciola are common among humans and livestock and can significantly impact sheep health. For this purpose, this study was conducted to determine the prevalence of hydatid cyst, Dicrocelium dendriticum and Fasciola spp. in slaughtered sheep in Khorramabad city during the second six months of 1401 and to investigate the environmental factors of temperature, rainfall and season on the incidence of these infections. During six months at the Gulshan Dam slaughterhouse, a total of 56,262 sheep underwent macroscopic inspection. Some infected samples were examined microscopically after being transferred to the laboratory. Information about age, sex, and infected organs was recorded through a prepared questionnaire. Prevalence of hydatid cyst, Dicrocoelium dendriticum, and Fasciola spp. in sheep was 12.43% (6996/56262), 7.63% (4295/ 56262), and 3.03% (1710/56262), respectively. The study revealed that hydatid cyst in both liver and lung was the most common parasitic infection found in the slaughtered sheep. Moreover, there was a statistically significant relationship between temperature and the prevalence of Dicrocoelium and hydatid cysts in the lung (p<0.05), as well as with season and rainfall. These factors showed a strong correlation with the incidence rate of Dicrocoelium and hydatid cysts. Climatic factors have a relative impact on the prevalence of platyhelminth infection in this city. In light of the relatively high prevalence of these platyhelminths in slaughtered sheep, policymakers, managers, and health experts need to develop an effective approach in this field.
Veterinary
Sheida Sadat Zonoori; Mohammad Fazilati; Hossein Salavati
Abstract
Today, Spirulina micro-algae is cultivated in many countries as a rich source of protein, unsaturated fatty acids, vitamins, minerals and antioxidants. In this study, the effect of replacing spirulina microalgae powder with different amounts of industrial vitamin-mineral premix was compared as an additive ...
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Today, Spirulina micro-algae is cultivated in many countries as a rich source of protein, unsaturated fatty acids, vitamins, minerals and antioxidants. In this study, the effect of replacing spirulina microalgae powder with different amounts of industrial vitamin-mineral premix was compared as an additive to stimulate the growth performance of broiler chickens. 200 one-day-old broiler chickens were randomly selected among 3000 chickens and divided into five treatments at Mehrgan Jihad Agricultural Research Center in Kermanshah province The diet of the first group, which was considered as a control treatment, includes (100% industrial supplement and no spirulina), the first treatment T1(25% spirulina and 75% industrial supplement), the second treatment T2 (50% spirulina and 50% industrial supplement), The third treatment T3 (75% spirulina and 25% industrial supplement) and the fourth treatment T4 (100% spirulina) were used. After 42 days, there was no mortality in the treatments, in the T1, T2 and T4 treatments, live weight significantly (P<0.05) increased compared to the control group, and the amount of feed conversion ratio (FCR) in the T2 treatment decreased significantly. Therefore, T2 showed the best performance Then the biochemical parameters of blood serum in T2 were checked, which showed a significant (P<0.05) increase in glucose, total protein and albumin, HDL and LDL and a decrease in triglyceride and cholestrol levels. Based on findings, it is concluded that by replacing 50% of the industrial vitamin-mineral supplement with spirulina powder, the growth performance, feed conversion ratio and blood parameters in broilers can be improved.
Veterinary
Jafar Pish Jang Aghajeri; Ghader Najafi; Parsa Pish Jang Aghajari; Fatemeh Mohammad Goli Famiyan; Elnaz Mehri
Abstract
Heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gene involved in heat stress (HSP90β) in Marandi indigenous, ...
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Heat stress causes significant economic losses in poultry production and leads to the reduction of several physiological and metabolic factors. This research was conducted in order to investigate the allelic and genotypic polymorphisms of the gene involved in heat stress (HSP90β) in Marandi indigenous, broiler and laying chickens using PCR-RFLP technique. Randomly, blood was taken from 300 chicken and genomic DNA was extracted by salting out method. Amplification of the desired gene locus with a length of 494 bp was performed using specific primers and MspI enzyme was used to identify the mutation in the desired gene locus. After enzymatic digestion, two genotypes M1M1 and M1M2 and two alleles M1 (with a band of 494 bp) and M2 (with two bands of 248 bp and 246 bp) were identified for the HSP90β marker locus. Marandi indigenous and broiler chicken masses were in the Hardy-Weinberg equilibrium. For Marandi indigenous and broiler chicken populations, the Shannon information index at the HSP90β marker locus was 0.25 and 0.40, the fixation index is -0.07 and -0.16, and the observed heterozygosity index was 0.14 and 0.28, respectively. Due to the presence of polymorphism and mutation in the studied gene locus, it can be used in the Marandi indigenous and broiler chickens by genetic selection with the help of this marker to eliminate heat-sensitive chickens and keep heat-resistant chickens.
Veterinary
Parvaneh Pakzad; Saleh Tabatabaei Vakili; Morteza Mamouei; Jamal Fayazi
Abstract
The present study was performed to investigate the effect of injection of PMSG and GnRH hormones on reproductive performance of Arabi ewes in the breeding season. 75 ewes were divided into 5 groups. The first group had no estrus synchronization program and no hormonal injection (control). Vaginal progesterone ...
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The present study was performed to investigate the effect of injection of PMSG and GnRH hormones on reproductive performance of Arabi ewes in the breeding season. 75 ewes were divided into 5 groups. The first group had no estrus synchronization program and no hormonal injection (control). Vaginal progesterone sponge (MAP) was inserted into other groups for 14 days. Simultaneously with the withdrawal of sponge, they were treated with: 1- injection of 500 IU of PMSG, 2- injection of 100 micrograms of GnRH, 3- injection of 500 IU of PMSG plus 100 micrograms of GnRH and 4- without injection (MAP only). One healthy and fertile Arabi ram was assigned to each of the 5 ewes. Estrous behavior of ewes was recorded for 7 days. The estrous response, return to estrus, pregnancy length, lambing rate, prolificacy rate, fecundity rate and the ratio of the birth of male and female lambs, were not affected by treatments. Injection of PMSG and GnRH were able to reduce the time of estrus onset coMAPred to sponge treatment alone (P<0.05). The birth weight and weight of 30, 60 and 90 days in lambs, although were affected by the treatments (P<0.05), but no obvious effect of hormone injection was observed on these parameters. In general, the injection of PMSG and GnRH in the estrus synchronization program of Arabi ewes, although was an effective method in estrus onset time, but could not have a significant effect on the other reproductive performances of the animal during the breeding season.
