Development
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; ...
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Human endometrial stem cells (hEnSCs) contain a cell population capable of self-renewal and show characteristic similar to mesenchymal stem cells. we show that these cells can differentiate to different cells .This work aimed to isolated hEnSCs from endometrial tissue of patients were extracted anzymaticaly; the released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometric analysis were done for CD105, CD90, CD146, CD31 and CD34 at third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated at the third passage after 21 days of induction.The flowcytometry analysis showed that EnSCs were positive for CD90, CD105, CD146 and were negative for CD31, CD34. Moreover we show that the hEnSCs can differentiate in osteocyte and adipocyte, as confirmed by alizarin Red and oil-Red O staining. These differentiated cells into neuron and endothelial express related markers including Nestin, MAP2 and CD31. Results show that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.
Development
Navid Dehnavi; Zohreh Moeini; Tahereh Foroutan
Abstract
Objective: Nanoparticles, owing to their unique physicochemical properties, have emerged as promising agents for biomedical applications, particularly in tissue engineering and regenerative medicine. Among the various types of nanoparticles, graphene oxide (GO) has garnered significant attention because ...
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Objective: Nanoparticles, owing to their unique physicochemical properties, have emerged as promising agents for biomedical applications, particularly in tissue engineering and regenerative medicine. Among the various types of nanoparticles, graphene oxide (GO) has garnered significant attention because of its biocompatibility and ability to facilitate cellular processes. This study investigated the role of graphene oxide nanoparticles in promoting neural differentiation of mouse mesenchymal stem cells (MSCs), focusing on the underlying mechanisms and outcomes of such interactions.
Materials and Methods: In this study, bone marrow-derived stem cells were isolated from the femurs of mice using a flushing method. The cells were cultured in three distinct groups for 14 days. Control Group: Cells were cultured in a neural differentiation medium without additives. Group 1: Cells were cultured in a general culture medium supplemented with 1.5 µg/ml of graphene oxide. Group 2: Cells were cultured in neural differentiation medium with 1.5 µg/ml of graphene oxide. To assess the effects of graphene oxide on cell viability and differentiation, MTT assays were employed to evaluate cytotoxicity, while immunocytochemistry (ICC) techniques were used to detect the expression of neural differentiation markers, including Sox2, β-tubulin III, and MAP2.
Results: The results demonstrated that both Group 1 and Group 2 exhibited expression of all three neural differentiation markers, Sox2, β-tubulin III, and MAP2, comparable to that of the control group. This indicates that the presence of graphene oxide, even in general culture medium, can promote neural differentiation. However, it is noteworthy that the dose of graphene oxide used in this study also exhibited no cytotoxic effects on the cells, suggesting a delicate balance between promoting differentiation and maintaining cell viability. The findings of this study underscore the potential of graphene oxide nanoparticles as a tool for enhancing neural differentiation of mesenchymal stem cells. The ability of GO to induce the expression of key proteins associated with neural differentiation without the need for additional nerve growth factors highlights its efficacy as a biocompatible scaffold.
Conclusion: This study provides evidence that graphene oxide nanoparticles can effectively promote the neural differentiation of mouse mesenchymal stem cells. The ability to induce the expression of critical neural markers through both direct cellular interactions and scaffold formation makes graphene oxide a valuable component in neuroregenerative strategies. Future research should focus on elucidating the precise mechanisms by which graphene oxide influences cellular pathways and optimizing its application in stem cell therapy for neurological disorders.
Development
Nasim Hayati; Amirhosein Fazlali; Gholamreza Kaka; Kazem Parivar
Abstract
Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were ...
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Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were divided into 4 groups of 7 Healthy rats, sciatic nerve cut rats without therapeutic intervention, rats with umbilical cord blood cells on PLGA membrane scaffold at the nerve cut site, rats were treated with umbilical cord blood cell injection at the injury site. The degree of recovery was evaluated by the sensorimotor activity of the sciatic nerve, electrophysiological studies.
Findings: motor evaluation of the sciatic nerve, the control group was not observed to return to the normal state in the eighth week, the cell therapy group was repaired on the PLGA membrane scaffold in the eighth week. The level of AMP in the 8th week after recovery of the cell therapy group with a gentle slope is a sign of the healing process of the cell therapy group. Counting the number of nerve fibers in a surface equal to 1000 square meters, the number of nerve fibers in the cell therapy groups increased after the eighth week of repair, compared to the control group and the PLGA membrane group. At the end of the eighth week, the sensory activity index of the sciatic nerve (Hot Plate test), the healing process of the cell therapy group on the PLGA membrane scaffold was more evident than the other groups.
Conclusion: Umbilical cord blood cell transplantation causes sciatic nerve repair and PLGA membrane scaffold with cord blood cells accelerates sciatic nerve repair.
Development
Faeze Zarean; Somayeh Arabzadeh; Sarah Rajabi; Saeideh Erfanian; Mahmood Talkhabi
Abstract
Bone is the hardest and one of the most important tissues in the body. In case of bone damage, the current treatments do not completely repair and regenerate the bone. For this reason, cell-based tissue engineering strategies, especially Mesenchymal Stem Cells (MSCs), have received attention. MSCs have ...
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Bone is the hardest and one of the most important tissues in the body. In case of bone damage, the current treatments do not completely repair and regenerate the bone. For this reason, cell-based tissue engineering strategies, especially Mesenchymal Stem Cells (MSCs), have received attention. MSCs have the ability to self-renew and differentiate into different cell types, including bone cells, cartilage cells, and fat cells, among others. They are found in various tissues throughout the body, including bone marrow, adipose tissue, and umbilical cord tissue. Today, MSCs are a valuable resource for regenerative medicine and tissue engineering applications. In addition to the cells, scaffolds are another essential element of tissue engineering. One of these scaffolds is decellularized tissue-derived hydrogels, which are three-dimensional network of hydrophilic polymer chains that can absorb and retain a significant amount of water. In tissue engineering, they mimic the natural extracellular matrix of tissues, providing a suitable environment for cells to attach, proliferate, and differentiate. In the current study, we aimed to investigate the effects of decellularized skeletal muscle-derived hydrogel, known as Myogel, on bone marrow-derived MSCs biological behaviors, including proliferation, viability and migration. In this study, MSCs were isolated from tibia and femur of adult Wistar rats. MSCs were cultured in a complete medium (a-MEM containing 15% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Pen/Strep)). The identity of cells was determined by morphology (using inverted microscope) and expression of specific CD markers (using Flowcytometry). Skeletal muscle was decellularized and accuracy of decellularization was evaluated using special staining. Then Myogel was prepared from digested decellularized skeletal muscle. Here, Myogel substrate was used as the control group, gelatin substrate as the positive control, and un-coated plates as the negative control. The effect of Myogel on survival (MTT method), proliferation (drawing the growth curve and calculating the doubling time of the cell population), cell cycle profile (flow cytometry method), and cell migration (scratch method) were investigated. The MTT test showed that the survival of MSCs in Myogel substrate with a concentration of 0.2 mg/ml was higher than the survival of MSCs in gelatin substrate with a concentration of 0.1 mg/ml and the survival of MSCs in gelatin was higher than the survival of control MSCs. Myogel substrate increased the proliferation and migration of cells and decreased the doubling time of MSCs population. Examining the cell cycle profile showed that a high percentage of cells cultured on Myogel were in the G1 and S phase of the cell cycle, indicating an increase in cell division speed by gelatin and, in the next degree, by Myogel. Therefore, Myogel can be used as a suitable substrate to increase the proliferative and migratory potential of MSCs, which are an important factor in tissue engineering.
Development
Fatemeh Ghiyasvand; Somayeh Arabzadeh; mahmood talkhabi
Abstract
Tissue engineering is an emerging field based on the three elements of cells, scaffolds, and bioactive molecules, and can be a useful method for treating muscle injuries. The aim of this study is to investigate the effect of ascorbic acid (AA) on the viability of bone marrow mesenchymal stem cells (BM-MSCs) ...
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Tissue engineering is an emerging field based on the three elements of cells, scaffolds, and bioactive molecules, and can be a useful method for treating muscle injuries. The aim of this study is to investigate the effect of ascorbic acid (AA) on the viability of bone marrow mesenchymal stem cells (BM-MSCs) cultured on skeletal muscle decellularization scaffold. First, BM-MSCs were extracted from rat leg bone marrow and cultured in vitro. The identity of the cells was assessed using flow cytometry. The extracted rat skeletal muscle was decellularized using a 1% SDS solution. The decellularization process was investigated by Masson Trichrome, and Alcian blue and DAPI staining.BM-MSCs were cultured on decellularized scaffolds and treated with 1 mM AA for 2 days. Then, the survival and viability of the cells were evaluated using scanning electron microscope (SEM) and MTT methods, respectively.BM-MSCs had a spindle morphology, and the results of flow cytometry showed the expression of CD44 and CD90 and the lack of expression of CD45 and CD34 in more than 90% of the cells. The staining verified the preservation of collagen and glycosaminoglycans and the absence of DNA in the decellularized tissue. MTT results showed that AA significantly increases the viability of BM-MSCs (P<0.05). Also, the SEM results showed that the cells in the group treated with AA were more proliferated. In general, AA can improve the efficiency of muscle tissue engineering by increasing the viability of BM-MSCs.
Development
Fatemeh Razeghi; Marjan Nouri; Fattah Sotoodehnejadnematalahi; Ehsan Ehsani; Kazem Parivar
Abstract
Kidney diseases are an important medical problem worldwide. Since there are limited treatment options for damaged kidneys, stem cell therapy has become an alternative treatment. The aim of present study is to investigate effect of culture medium obtained from mesenchymal stem cells (MSCs-CM) of newborn ...
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Kidney diseases are an important medical problem worldwide. Since there are limited treatment options for damaged kidneys, stem cell therapy has become an alternative treatment. The aim of present study is to investigate effect of culture medium obtained from mesenchymal stem cells (MSCs-CM) of newborn mice kidneys in percentages of 10, 30 and 50 on differentiation of embryonic stem cells towards kidney epithelial cells. Mesenchymal stem cells were isolated from kidneys of newborn mice, passaged and propagated. Determination of the identity of cells was done using flow cytometry and checking the expression of surface markers CD105, CD29, CD90. In third passage of extracted cells, supernatant culture medium was collected, hESC were cultured and multiplied in the complete culture medium of cells and the differentiation of hES cells into progenitor cells was investigated. The expression of PAX2, ZO1 and CK18 genes was investigated using RT-PCR, expression of CD133, CD24 and CD44 surface markers was investigated using flow cytometry. Flow cytometry results confirmed the mesenchymal nature of the cells. The results of differentiation of hESCs showed that expression of PAX2, ZO1 and CK18 genes increased significantly (p<0.05) in the groups containing supernatant. The results of flow cytometry show an increase in expression of CD133 and CD24 markers in groups containing CM and the expression of CD44 marker in the group containing 50% CM, compared to control group. In general, results showed supernatant culture process of cells has a positive effect on inducing differentiation of human embryonic stem cells into kidney progenitor cells.
