Seyyedeh Sara Sadri Jokndan; Sohrab Rasouli
Abstract
Sarcocystis is a intracellular parasitic protozoan that can cause gastrointestinal disorders in patients and huge financial losses in the livestock industry. This study was performed to determine the prevalence of Sarcocystis infection in slaughter buffaloes in Urmia slaughterhouse, Iran. This study ...
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Sarcocystis is a intracellular parasitic protozoan that can cause gastrointestinal disorders in patients and huge financial losses in the livestock industry. This study was performed to determine the prevalence of Sarcocystis infection in slaughter buffaloes in Urmia slaughterhouse, Iran. This study was observed during a six-month period (October to March 2016) at 10-day intervals by referring to Urmia slaughterhouse and preparing carcasses of various tissues including tongue, esophagus, heart, diaphragm, thigh, and arm for the presence of rice grain cysts. During the present study, a total of 120 buffalo carcasses were examined macroscopically. For microscopic examination by digestion method, 100 g of each animal tissue was packed and transferred to the parasitology laboratory of the Faculty of Veterinary Medicine, Urmia Azad University. According to the results, no macrocysts were observed in buffaloes. Microscopic examination showed a moderate frequency of infection with Sarcocystis microcysts that 16.67% of the studied buffaloes were found to be positive for infection. Data analysis showed that there was a statistically significant difference between the rate of infection in different age groups and the rate of infection increased with age (p <0.05), while the rate of infection was gender independent and there was no significant difference between the rate of infection of different sexes (p>0.05). There was also a statistically significant difference between the level of infection in different muscles (p<0.05). All skeletal and esophageal muscles had microcysts and then microcysts were observed in the diaphragm, tongue, and heart muscles. our study showed that digestion is one of the most useful and accurate methods available to identify infected samples.
Khalideh Azari; Abdolghaffar Ownagh; Karim Mardani
Abstract
The present study aimed to identify fimbria, fluroquinolone and extended-spectrum beta-lactamase (ESBLs) resistant genes in Escherichia coli isolated from buffalo feces. In this study, a number of 384 buffalo feces from different regions of west Azerbaijan and in different seasons were randomly collected. ...
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The present study aimed to identify fimbria, fluroquinolone and extended-spectrum beta-lactamase (ESBLs) resistant genes in Escherichia coli isolated from buffalo feces. In this study, a number of 384 buffalo feces from different regions of west Azerbaijan and in different seasons were randomly collected. Fecal samples were cultured and E. coli were investigated using biochemical method. Polymerase chain reaction was employed to identify fimbria genes (fimA, crl and csgA), tsh gene, floroqinulone (qnrA, qnrB, qnrS) and extended-spectrum beta-lactamase (blaSHV, blaTEM, blaCTX-M) resistance genes. A number of 115 (29.9%) fecal samples were positive for E. coli. The frequency of the positive fecal for E. coli from northern region were significantly lower than central and southern regions (P<0.05). The frequency of positive fecal samples for E. coli did not differ between seasons. The frequency of fimbria genes fimA, crl and csgA were 79.1%, 72.1% and 74.7% respectively. tsh gene had the lowest frequency in E. coil isolates. Among fluroquinolone and extended-spectrum beta-lactamase resistance genes, qnrS gene had the lowest (6.0%) and blaTEM had the highest (13.9%) frequencies. The results revealed that E. coli was isolated from less than one third of fecal samples. Fimbria genes had almost similar frequencies among E. coli isolates and antibiotic resistance genes were exist in less than 14% of E. coli isolates from buffalo feces. The investigation of antibiotic resistance genes in E. coli originated from animals is of great epidemiological and public health importance.
