Articles in Press, Accepted Manuscript, Available Online from 20 February 2015
Abstract
Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called molecular ...
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Abstract The altered or mutated forms of genes known as proto-oncogenes are responsible for promoting cell growth and uncontrolled cell proliferation. An accumulation of many mutations in different and specific genes,over time is required to cause cancer. The pattern of gene expression, also called molecular signature is unique to a particular class of tumor or tumor cell. This paper describes the latest technique for monitoring the expression of a panel of cancer-specific genes. The PCR technique combines the quantitative performance of SYBR® Green-based real-time PCR is widely used for gene profiling. This technique is cost-effective, easy-to-use, and focuses only on the genes that you desire. In this study the expression of our target genes were quantitatively determined in five human cancer cell lines. We selected gene β-actin as our reference gene. Cells were lysed and the mRNAs were extracted using the RNA Purification Kit and cleaned up with Qiagen RNeasy spin columns. The first-strand cDNA was synthesized according to the High Capacity cDNA Reverse Transcription Kit protocol. RT-PCR were performed with Gene Expression Assays in an AB step one plus Sequence Detection System. Briefly the expression of p53 was high in both breast cancer cell lines, MCF7, T47-D and lung cancer cells, A549. Src expression was higher in prostate cell line, PC3 and lung cancer cells, A549. Meanwhile SKOV3 (ovarian cancer cell line) showed high expression of her-2 gene. The results clearly show that the expression pattern of this panel of genes was unique to almost every cell line examined.
Fisheries
seyedeh abedi; mohammad khalesi; Sohrab Eskandari
Articles in Press, Accepted Manuscript, Available Online from 01 December 2013
Abstract
This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity ...
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This research aimed at comparison of chromium bioaccumulation patterns in skin, gills, scales, liver and muscle of two widely-consumed fishes: scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus. First, lethal concentration (96 h LC50) of chromium as well as its toxicity factor (TF) was determined for each species. For bioaccumulation trial, the two species exposed to sublethal concentrations (10% of LC50 values) of Cr at separate triplicate treatments for 15 days. The examined tissues from each species were sampled at days 7 and 15 of the experiment. Following chemical digestion, each tissue was analyzed by atomic absorption unit (Model Thermo) to measure chromium concentration. According to the results, Almost all differences in bioaccumulations between the two species and the controls were significant (p < 0.05). Concentration of chromium in common carp was in the form of gills> liver> skin> scales> muscle, and accumulation in catfish was in the form of liver> muscle> skin> gills. Final entire contents of Cr in the catfish's tissues were 3.65 times, than in the carp’s (excluding the scales). In addition, the catfish's muscle amassed 16.15 times greater amounts of Cr.The results of this investigation indicate that the carp's scaled armature, compared to the scaleless skin of the catfish, adsorbs metal toxicants leading to much lesser metals content in the muscle rendering this tissue rather safer for comestible consumption.
Hajimohammad Shirmohammadli; Majid Mohammadnejad
Abstract
Environmental, nutritional, size and age conditions cause many direct and indirect changes in the blood and serum indices of fish in different species. The aim of this study was to determine and evaluate the changes in hematological indicators and some biochemical indicators of Cyprinus carpio blood ...
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Environmental, nutritional, size and age conditions cause many direct and indirect changes in the blood and serum indices of fish in different species. The aim of this study was to determine and evaluate the changes in hematological indicators and some biochemical indicators of Cyprinus carpio blood serum in different weights. For this purpose, common carp farmed in the weight range of 500 to 2000 g were examined and analyzed in 5 weight groups of 500, 700, 1000, 1300 and 1600 g. The studied fish were bred and examined in a 2-hectare pool in one of the farms of Sari city under the same conditions. To perform blood tests, 5 fish were used in each weight group and blood sampling was performed by cutting the vein of the caudal stem vein. The results of the study of hematological indicators and some serum biochemical indicators showed that in different weights, significant changes in the amount of red blood cells, hemoglobin, hematocrit, mean red blood cell volume, mean hemoglobin concentration, mean red blood cell hemoglobin concentration, white blood cells, lymphocyte, monocyte, granulocytes, glucose, urea, triglycerides, cholesterol, phosphorus, calcium, sodium, potassium, albumin, and total protein were not observed (P> 0.05). The results of the present study showed that weight changes did not affect the hematological indicators and some biochemical indicators of common carp blood serum.
Elham Madani; azam moshfegh; Mahbubeh Setorki; Akram Tehranifard
Abstract
Glyphosate based pesticides are safe for humans, but their widespread use in agricultural areas and water contamination raised serious concerns about its toxicity. The aim of this study was to investigate the effects of different doses of roundup on body weight, gonadosomatic index (GSI) and histological ...
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Glyphosate based pesticides are safe for humans, but their widespread use in agricultural areas and water contamination raised serious concerns about its toxicity. The aim of this study was to investigate the effects of different doses of roundup on body weight, gonadosomatic index (GSI) and histological alterations of gill and testis in common carp. 32 common carp broodstock (1723.75 ± 72.72gr) were randomly divided into 4 groups of 8 fish. . One group (control) had no exposure to herbicide and 3 groups (treatment) were exposed to rounduppesticide at 4 concentrations (5, 10 and 15 mg/L) for 10 days. After treatment period, body length, body weight and GSI index were determined and gill and testis samples were prepared for histological studies. Based on the results, exposure to roundup at a dose of 20 mg/L caused a significant decrease in body weight on the10th day and GSI index on the 5th and 10th day (p<0.05). In fishes exposed to roundup (20mg/L) necrosis and disintegration of gill lamellae structure, adhesions of gill lamellae, separation of basement membrane and damage of gill epithelium and exudation of blood cells were observed. In the testis of fish exposed to the highest dose of roundup, an increase in the number of spermatogonium due to reduced spermatogenesis, degradation of spermatocyte, reduction of spermatozoa, reduction of sperm storage tubules diameter and destruction of interstitial duct and sertoli cells were observed. The results indicated destructive effects of sublethal doses of roundup on gill structure and reproductive performance of common carp.
Seraj Bita; Mehrzad Mesbah; Ali Shahryari; Masood Ghorbaanpoor Najafabad
Abstract
Abstract In this study, in order to investigate the antibacterial properties of silver nanoparticles that were synthesized using Sargassum seaweed extract by biological extracellular method, Mesophile Enterobacteriaceae, lactic acid bacteria and psychrophilic bacteria skin of common carp exposed ...
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Abstract In this study, in order to investigate the antibacterial properties of silver nanoparticles that were synthesized using Sargassum seaweed extract by biological extracellular method, Mesophile Enterobacteriaceae, lactic acid bacteria and psychrophilic bacteria skin of common carp exposed to three concentration (0.11, 1.13, 5.67 mg/L AgNP) of this silver nanoparticles were studied for 14 days. The results showed that with increasing concentration of silver nanoparticles, the load of Enterobacteriaceae and Mesophile significantly reduced compared with control (p0.05). The lowest bacterial count was related to enterobacteria (log cfu/cm2 1.00±0.01), when exposed to concentration 1.13 mg/L AgNP. Lactic acid bacteria in all treatments and in control, did not grow until the last day of exposure.
Morteza Kamali; Saeid Alinejad Moaelem; Nastaran Pour-Ahmadi
Abstract
Abstract Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and ...
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Abstract Due to its suitable properties and the low cost; Di 2-Ethyl Hexyl phthalate (DEHP) is widely used as a plasticizer in manufacturing of articles made of PVC. This substance is of paramount importance with the estrogen-like properties and its effects on the survival and health of ecosystems and the aquatic and human populations. In present study; effects of toxicity of DEHP on protein band model and amino acid profile of mucus common carp (Cyprinus carpio) was studied. 108 common carp (17.60±2.22gr) is gathered from warm water fish center and they were allocated into 9 tanks (12 Fish per tank). Fish adapted to new condition for a week. During this time; they were feed twice a day with same diets. Fishes were exposed at 3 concentration treatments containing 0; 0.1 and 1 mg/L DEHP under laboratory condition (25±0.5°C; pH: 7.4-8) for a period of 96 hours. We assessed skin mucus protein profiles by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and we were studied amino acid profile by HPLC. The results of the experiments to compare the protein pattern of carp’s mucus (exposed to DEHP with two different concentrations) with the control sample (without exposure to DEHP) showed that there is a significant difference in the bands density between the two protein patterns. These results indicated that concentrations of DEHP beneficially affects Amino Acid profiles of skin mucus in carp and they were categorized in 4 branches. There were Significant differences between exposed and control groups. Therefore; fish skin mucus can be a biomarker for showing contaminants and toxins. And the high consumption of plastic materials can be a warning to the health of aquatic animals and humans.
Mohammad Forouhar Vajargah; Seyed Aliakbar Hedayati
Abstract
Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor ...
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Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor poison that is a very common at the agricultural plant was studied in 5-7 gr common carp and Caspian roach for detection of LC50 at 96 hour. Experiment was in static plan and based on the OECD standard conducted for 4 days; water physicochemical parameters including PH, dissolved oxygen and temperature were measured that they were in suitable ranges. Based on the results, acute toxicity of butachlor on carp and Caspian roach were 0.785 and 0.258 mg/l respectively and maximum allowable concentration MAC value were calculated as 0.078 and 0.025 mg/l respectively. According to the standard for determine of the toxicity of various pesticides, butachlor was considered more highly toxic for Caspian roach than common carp.
