Biochemistry
Asieh Emami Nejad; Mohammad Fazilati; Hossein Salavati; Habiballah Nazem
Abstract
The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine ...
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The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine protease enzymes using selenium and calcium nanoparticles was presented. Due to its high biocompatibility, toxicity, and low cost, these nanoparticles have become one of the most prominent non-metallic oxide nanoparticles, which, with their antioxidant and anti-inflammatory properties, can protect against the destruction of enzymes by free radicals. The results showed that these nanoparticles can significantly increase the half-life of these enzymes. On the other hand, the serine protease enzyme immobilized on selenium nanoparticles has unique biochemical characteristics in the presence and absence of calcium. The obtained results indicate that the stabilized enzyme has high activity and stability in a wide range of temperatures and pH compared to pure and impure-free enzyme. The results showed that the enzyme immobilized on selenium nanoparticles increased its activity by more than 99% in the presence of calcium at the temperature of 45°C. On the other hand, the half-life of the free enzyme increased from 95 minutes in the pure form to 115 minutes in the form immobilized on nanoparticles in the presence of calcium. These results indicate that the presence of nanoparticles can prevent the destruction of enzymes by free radicals.
behrrouz heidari; Mina Ahmadpour; Razyeh Amani
Abstract
Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated ...
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Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health.
