Mohammad Forouhar Vajargah; Hamed Ghafari Farsani; Saeid Shahbazi Naserabad; Aliakbar Hedayati
Abstract
Abstract Different organisms like aquatics are constantly exposed to pesticide residues, this study's overall assessment patho-biological changes in gills and livers of Capoeta capoeta gracilis during exposure to butachlor pollutant. First of all the 96-hour lethal concentration of the toxin was calculated ...
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Abstract Different organisms like aquatics are constantly exposed to pesticide residues, this study's overall assessment patho-biological changes in gills and livers of Capoeta capoeta gracilis during exposure to butachlor pollutant. First of all the 96-hour lethal concentration of the toxin was calculated as 46.2 mg per liter. Then, 96 fish were divided into four groups: one control group and three experimental groups (with concentrations of 0.2, 0.4, and 0.8 mg l-1) and were exposed for 2 weeks. In the end point, histological changes were studied by hematoxylin and eosin staining method in light microscopy. Lethal concentration of this toxin was 2.46 mg/l that means moderate toxicity of this species. There was many histological changes in the gills of fish treated with butachlor include: hyperplasia, shortening the secondary lamella, edema, fusion, hemorrhage and atrophy in compared to the control group. Treatment with liver butachlor cause obstruction, dark granules, necrosis, and bile stagnation and sinusoid dilution in studied fish. The results of this study showed that in both studied organs with increase of toxin concentration, more severe tissue damage was observed, so gill and liver tissues of this fish could be used as an appropriate biomarker for pollutant measurement.
Mohammad Forouhar Vajargah; Seyed Aliakbar Hedayati
Abstract
Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor ...
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Abstract One of the most important factors that today is important with the risks associated of the growing industry and technology, is plant pesticides that can lead to the destruction of aquatic communities in the long times by entering aquatic ecosystems. So in current study acute toxicity of butachlor poison that is a very common at the agricultural plant was studied in 5-7 gr common carp and Caspian roach for detection of LC50 at 96 hour. Experiment was in static plan and based on the OECD standard conducted for 4 days; water physicochemical parameters including PH, dissolved oxygen and temperature were measured that they were in suitable ranges. Based on the results, acute toxicity of butachlor on carp and Caspian roach were 0.785 and 0.258 mg/l respectively and maximum allowable concentration MAC value were calculated as 0.078 and 0.025 mg/l respectively. According to the standard for determine of the toxicity of various pesticides, butachlor was considered more highly toxic for Caspian roach than common carp.
