Elham Madani; azam moshfegh; Mahbubeh Setorki; Akram Tehranifard
Abstract
Glyphosate based pesticides are safe for humans, but their widespread use in agricultural areas and water contamination raised serious concerns about its toxicity. The aim of this study was to investigate the effects of different doses of roundup on body weight, gonadosomatic index (GSI) and histological ...
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Glyphosate based pesticides are safe for humans, but their widespread use in agricultural areas and water contamination raised serious concerns about its toxicity. The aim of this study was to investigate the effects of different doses of roundup on body weight, gonadosomatic index (GSI) and histological alterations of gill and testis in common carp. 32 common carp broodstock (1723.75 ± 72.72gr) were randomly divided into 4 groups of 8 fish. . One group (control) had no exposure to herbicide and 3 groups (treatment) were exposed to rounduppesticide at 4 concentrations (5, 10 and 15 mg/L) for 10 days. After treatment period, body length, body weight and GSI index were determined and gill and testis samples were prepared for histological studies. Based on the results, exposure to roundup at a dose of 20 mg/L caused a significant decrease in body weight on the10th day and GSI index on the 5th and 10th day (p<0.05). In fishes exposed to roundup (20mg/L) necrosis and disintegration of gill lamellae structure, adhesions of gill lamellae, separation of basement membrane and damage of gill epithelium and exudation of blood cells were observed. In the testis of fish exposed to the highest dose of roundup, an increase in the number of spermatogonium due to reduced spermatogenesis, degradation of spermatocyte, reduction of spermatozoa, reduction of sperm storage tubules diameter and destruction of interstitial duct and sertoli cells were observed. The results indicated destructive effects of sublethal doses of roundup on gill structure and reproductive performance of common carp.
Behrooz Heidari; Sanaz Avarjeh; Hasan Taghavi Jelodar
Volume 4, Issue 2 , December 2015, , Pages 25-35
Abstract
Environmental factors including salinity and temperature can affect the physiology and performance of the growth of fish, so many of internal organs such as gill may be showed wide range of morphological and functional changes. Fingerling common carp were exposed for 21 days in groups at 20, 25 ...
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Environmental factors including salinity and temperature can affect the physiology and performance of the growth of fish, so many of internal organs such as gill may be showed wide range of morphological and functional changes. Fingerling common carp were exposed for 21 days in groups at 20, 25 and 30°C and three salinity levels 0, 5 and 10ppt. Histological changes of gills tissue of common carp with average weight and length 38.28 ± 0.58 g and 14.04 ± 0.10 cm respectively is studied. After biometry, sampling was performed on 7 and 21 and transferred to the laboratory. The gill arc was fixed in Bouin's solution for 24-48 hours. To evaluate the chloride cells in the gill tissue of the fish, PAS-hematoxylin were used. The results showed that the size and number of chloride cells increased by increasing the temperature and salinity. The maximum number and size of chloride cells was observed at treatment with temperature 30 °C and salinity 10ppt. In conclusion, it could be stated that the number and size of chloride cells in the gill were affected by combined environmental factors, salinity and temperature.
A Louei Monfared; A Kalili; L Khalili; M.R Kalbasi
Volume 3, Issue 3 , May 2015, , Pages 13-22
Abstract
Abstract Present work investigates the effects of nanosilver administration on histology of gill by direct and indirect methods in rainbow trout larva. In the direct mode, 300 larvae were divided to 6 equal groups randomly and exposed to 0.015, 0.031, 0.062, 0.125, 0.25 and 0.5 ppm over 25 days. In the ...
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Abstract Present work investigates the effects of nanosilver administration on histology of gill by direct and indirect methods in rainbow trout larva. In the direct mode, 300 larvae were divided to 6 equal groups randomly and exposed to 0.015, 0.031, 0.062, 0.125, 0.25 and 0.5 ppm over 25 days. In the indirect mode, 50 larvae were exposed to zeolite covered by silver nanoparticles. In addition, 50 larvae were kept in the incubators which free of any additive nanomaterials as control. In all of the experimental groups, on the days 4, 8, 12 and 25 after treating tissue samples were taken and histological alterations of the gill were examined. In the gill tissues of the direct exposed to nanosilver as 0.062 and greater concentrations; severe histoloogical and histometrical alterations include aneurism in the secondary lamellae of gills, hyperplasia of epithelium of gills as well as the adhesion of the gill lamellae were seen, as compared with control animals. In addition, in the direct exposing method; any increasing in the silver nanoparticles concentrations could lead to significant elevation in the gill lamellar as well as filament diameters (p≤0.05). Furthermore, indirect exposure to silver nanoparticles could not affect the gill integrity. It is concluded that nanosilver administration in the larval stage of the trout must be taken by zeolite covered by silver nanoparticles.
