behrrouz heidari; Mina Ahmadpour; Razyeh Amani
Abstract
Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated ...
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Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health.
Jafar Ehsani Kenari; Abolghasem Esmaeili Fereidouni; Masoud Farokhrouz Lashidani
Volume 4, Issue 1 , September 2015, , Pages 53-65
Abstract
Abstrac Effects of stress due to transportation on the common carp (Cyprinus carpio) broodstock from ponds to the hatcheries in three intervals include immediately after the catching, 2 hours for transfer and 24 hours after transportation on changes in levels of cortisol, glucose, sex steroids (testosterone ...
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Abstrac Effects of stress due to transportation on the common carp (Cyprinus carpio) broodstock from ponds to the hatcheries in three intervals include immediately after the catching, 2 hours for transfer and 24 hours after transportation on changes in levels of cortisol, glucose, sex steroids (testosterone and estradiol) and blood factors were analyzed with sampling from 30 broodstock. Results showed that cortisol levels rose quickly after the catching (625 ng per ml) and significantly decreased after 24 hours (409 ng per ml) (P0.05). Testosterone and estradiol levels were significantly different among the three intervals, in which the highest values were observed after the catching and significantly decreased with time (P0.05). Also, 24-hour maintenance of breeder’s improved the blood parameters. Based on the results, the transportation of common carp broodstock had negative effects on cortisol, steroid hormones and some blood parameters (erythrocyte and hemoglobin values), thereby it is necessary to the maintenance of broodstock (even in the short-term transfer) in hatcheries ponds.
