Biochemistry
Ali Ahmadi Shapourabadi; Mohammad Fazilati; Habib-Allah Nazem
Abstract
Lactoferrin is one of the milk proteins that has shown a wide range of physiological activities such as antibacterial, anti-protozoal, anti-fungal, anti-viral, anti-cancer, antioxidant, anti-inflammatory and immunomodulatory. In this study, lactoferrin was extracted and purified from cow, sheep and goat ...
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Lactoferrin is one of the milk proteins that has shown a wide range of physiological activities such as antibacterial, anti-protozoal, anti-fungal, anti-viral, anti-cancer, antioxidant, anti-inflammatory and immunomodulatory. In this study, lactoferrin was extracted and purified from cow, sheep and goat milk. HPLC analysis and determination of the concentration of these three extracted lactoferrins were performed. Antibacterial activity against Gram negative Escherichia coli and Salmonella typhi and gram positive Bacillus cereus and Staphylococcus aureus and antioxidant activity were investigated. The activity of lactoferrin against DPPH free radical was investigated. Also, lactoferrin activity was evaluated in neutral acidic and alkaline environments. The combination test with iron was also performed with FeNTA reagent. The findings showed that lactoferrin has the ability to inhibit two bacteria, Escherichia coli and Staphylococcus aureus. Also, the evaluation of lactoferrin activity in neutral acidic and alkaline environments showed that lactoferrin is more stable in alkaline environment than other environments. In the combination test with iron, the results indicated the combination of lactoferrin with iron. The percentage of free radical inhibition was equal to %4.49. Also, the results showed that the concentration of lactoferrin in goat's milk is higher than that of sheep's and cow's milk and its amount was equal to 131.66 μg/ml.
A. GH. OWNAGH; N. AGH; H. AZIZI; B. H. RAHMAN
Volume 3, Issue 2 , March 2015, , Pages 21-30
Abstract
Use of growth and feeding stimulators have proven to have an important role in the development of aquaculture industry. In this study, we investigated the effect of Lactobacillus plantarum (L. plantarum) and lactoferrin on growth and feeding indices of rainbow trout (Oncorhynchusmykiss). 900 fish (average ...
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Use of growth and feeding stimulators have proven to have an important role in the development of aquaculture industry. In this study, we investigated the effect of Lactobacillus plantarum (L. plantarum) and lactoferrin on growth and feeding indices of rainbow trout (Oncorhynchusmykiss). 900 fish (average weight 20±2 g) were divided into 6 feeding treatments supplemented with different doses of L. plantarum and lactoferrin and were fed for two months. Growth and feeding parameters including wet weight (wet W), standard length (SL), feed conversion ratio (FCR), specific growth rate (SGR) and condition factor (CF) were measured on days 0, 15, 30, 45 and 60 and compared with the control group. The results showed that treatments receiving diets containing L. plantarum 108cfu/kg and treatments that received 200 mg/kg lactoferrin in diet had significantly higher growth compared to the control group (pL. plantarum and lactoferrin did not show any reinforcing effect on feeding and growth parameters. It seems that supplementation of 108cfu of L. plantarum and 200 mg of lactoferrin per kg of diet, alone, is more effective and less expensive way to enhance growth and feeding indices in rainbow trout compared to their combined use.
