Homayoun Khazali; Nazli khajehnasiri; Farzam Sheikhzadeh Hesari
Abstract
Abstract Location of two sets of neurons; pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus, represents a core role of the hypothalamus in the regulation of energy balance. One of the important factors in energy balance is exercise. Until now, the effects ...
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Abstract Location of two sets of neurons; pro-opiomelanocortin (POMC) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus, represents a core role of the hypothalamus in the regulation of energy balance. One of the important factors in energy balance is exercise. Until now, the effects of exercise on hypothalamic POMC and NPY genes expression, weight and appetite control are not clear. Therefore, in the present study, the influence of one-month regular moderate exercise in serum level of corticosterone, as one of the factors affecting appetite, and gene expression of hypothalamic POMC and NPY were discussed.Fourteen adult male Wistar rats were randomly divided into control and one-month regular moderate exercise (RME) groups. After, one-month exercise (22m/min), the arcuate nucleus was isolated from brain and stored in -80 refrigerators for gene expression assay by real-time PCR method. In addition, serum samples were taken to assess corticosterone level by ELISA method. Data were analyzed by Independent t-test in SPSS-16. Pro-opiomelanocortin mRNA level was lower in the group which received exercise less than the control animal (P. The results of the present study indicate that regular moderate exercise is effective in reducing stress but probably increasing anorexigenic neuropeptide, proopiomelanocortin, gene expression is the reason for incapacitation of exercise for losing weight.
Farzam Sheikhzadeh Hesari; Nazli Khajehnasiri
Volume 5, Issue 1 , September 2016, , Pages 83-91
Abstract
A cardiovascular problem is one of the common complications of diabetes. Connexin is the predominant protein subunit in gap junction structure which is important in communication between cells. Considering the role of connexin, this question can be created in the mind whether changes in phosphorylated ...
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A cardiovascular problem is one of the common complications of diabetes. Connexin is the predominant protein subunit in gap junction structure which is important in communication between cells. Considering the role of connexin, this question can be created in the mind whether changes in phosphorylated connexin 43 occurs in diabetics thoracic aorta or not? 20 male Wistar rats (30050g) were randomly divided into two groups (n=10): control and diabetic group. Diabetes mellitus was induced by intraperitoneal injection of streptozotocin (60 mg/Kg). After 60-days, rats were anaesthetized and then thoracic aorta was isolated and immediately Freeze, at the end phosphorylated connexin 43 levels were measured by ELISA method. In this study, a significant increase in the level of connexin 43 is observed in the diabetic group (P
