Biochemistry
Pooyan Pedram; Mohammad Fazilati; Marzieh Rashidipour; Habibollah Nazem
Abstract
Lactate dehydrogenase (LDH) is a key enzyme in cellular metabolism found in all animals. It plays a crucial role in converting pyruvate to lactate and vice versa. LDH is present in a wide range of tissues and cells in the animal body. In recent decades, nanoparticles have been utilized due to their unique ...
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Lactate dehydrogenase (LDH) is a key enzyme in cellular metabolism found in all animals. It plays a crucial role in converting pyruvate to lactate and vice versa. LDH is present in a wide range of tissues and cells in the animal body. In recent decades, nanoparticles have been utilized due to their unique properties for designing optical and electronic sensors. This research presents a novel colorimetric method: silver nanoparticles synthesized using chrysanthemum aqueous extract are employed for direct detection of lactate dehydrogenase activity. Initially, chrysanthemums were collected from greenhouses in Mahallat County under the supervision of experts. After separation and powder preparation of the flower part of the plant, chrysanthemum aqueous extract was prepared. Subsequently, the synthesis of silver nanoparticles using aqueous extract and addition of silver nitrate solution was investigated by optimizing appropriate conditions. In the next step, two vials were prepared, each containing a reaction mixture comprising Tris-HCl, MgCl2, and NADH. Additionally, one vial contained LDH. Silver nanoparticles and sodium borohydride were then added to the vials. The enzyme can convert NAD+ to NADH. The detection mechanism of lactate dehydrogenase enzyme is based on the aggregation of silver nanoparticles, which leads to an increase in their size and consequently a color change. Thus, the presence or absence of the enzyme can be easily distinguished with the naked eye in a single step. In the presence of the enzyme, the color of the solution used in the study was yellow, while in the absence of the enzyme, the color was grayish. Consequently, lactate dehydrogenase enzyme can be identified with high sensitivity.
Leyla Najafi; Mohammad Fazilati; Hossein Salavati
Abstract
C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various ...
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C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes.
Fatemeh Nabipour; Mohammad Fazilati; Behroze Dousti; Reza Mir Derikvand
Abstract
Recently, due to increased Candida infection and frequent recurrence and due to the resistance of Candida species to most antifungal drugs, for the first time, antifungal properties of methanolic, n-hexane and aqueous extracts from different parts of Echium italicum were compared with antibiotics. In ...
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Recently, due to increased Candida infection and frequent recurrence and due to the resistance of Candida species to most antifungal drugs, for the first time, antifungal properties of methanolic, n-hexane and aqueous extracts from different parts of Echium italicum were compared with antibiotics. In this study, methanol, n-hexane and aqueous extracts from different parts of the plant were prepared in different concentrations by soaking method And on two species of Candida glabrata and Candida tropicalis by disk diffusion method and MIC (minimum inhibitory concentration), MFC (minimum fungicidal concentration of growth) by micro dilution method. Antibiotics Nystatin and Fluconazole were used as a positive control and DMSO was used as a negative control. Data were analyzed by one-way ANOVA and t-test. The results showed that different extracts from the root of the plant showed more antifungal activity than the aerial part of the plant against both species of Candida (P<0/05). The methanolic extract of the root showed the highest mean diameter of growth and the lowest MIC (15.62 μg/μl) and MFC (31.25 μg/μl) against Candida glabrata as inhibitory diameter of growth the methanolic extract in concentration of 5mg/ml (18.5±0.5) was higher than Nystatin antibiotic inhibitory diameter of growth for Candida glabrata. The results showed that the extracts of this plant have antifungal effects on both Candida species and after supplementary and clinical trials as an appropriate antifungal agent against Candida glabrata infection and Tropicalis infection.
Gholamreza Mahdieh najafabady; Mohammad Fazilati; Aliasghar Pilehvarian; Habib olah Nazem
Abstract
Abstract High blood pressure doubles the risk of cardiovascular disease. In addition to chemical drugs, biodegradable peptides have antihypertensive properties and the Ile-Gln-Pro peptide is one of these peptides. Spirulina is a green-blue algae of the cyanobacteria branch, which is now used as a supplement ...
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Abstract High blood pressure doubles the risk of cardiovascular disease. In addition to chemical drugs, biodegradable peptides have antihypertensive properties and the Ile-Gln-Pro peptide is one of these peptides. Spirulina is a green-blue algae of the cyanobacteria branch, which is now used as a supplement to the pharmaceutical industry. The Ile-Gln-Pro peptide was extracted from Spirulina platensis algae using an alkalase enzyme and was analyzed by filtration gel chromatography and RP-HPLC and its inhibitory effect on the ACE enzyme was investigated. ACE enzyme was inhibited by Ile-Gln-Pro peptide by non-competitive inhibition method with Ki = 5.8 μM and IC50 = 0.65 μM . According to the available information from the ACE inhibitor peptide in the BIOPEP database, a comparative study was performed on the location of amino acids in the ACE inhibitor peptide and its effect on the potency of tri-peptide inhibition. Based on SPSS software, on the 196 ACE inhibitor peptide, the presence of Lys, Gly, Thr and Pro amino acids at the end of C and His, Ala, and Met amino acids at the end of N, as well as Phe, Ala, His And His in the middle of peptide have relationship with EC50 and peptide-inhibiting potency.
