Bibi Fatemeh Fani Yazdi; Esmail Fattahi; Naser Mahdavi Shahri; Mohammad Mahdi Ghahramani Seno
Abstract
The interaction between ECM and cells plays an important role in the direction of cellular behavior. So far, different scaffolds have been developed to examine the behavior of cells in three-dimensional conditions. The aim of this study was to investigate the interaction between the mesenteric decellularized ...
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The interaction between ECM and cells plays an important role in the direction of cellular behavior. So far, different scaffolds have been developed to examine the behavior of cells in three-dimensional conditions. The aim of this study was to investigate the interaction between the mesenteric decellularized scaffold of the bovin and the blastema tissue originated from the pinna of New Zealandwhite rabbit. Mesenteric bovine tissue (5 mm×5 mm) was decellularized using physical methods (slow freezing and snap freeze–thaw) and chemical agents (sodium dodecyl sulfate (SDS) and Triton Х-100) followed by washing and sterilization procedures.These parts were assembled as scaffolds inside the blastema rings from rabbit’s pinna. Sampling was carried out on days 7, 10, 15 and 21. Interactions between the scaffolds and the blastema tissue cells were studied by histological and fluorescence microscopy and electron microscopy.The study of the behavior of Blastema cells in different days of culture in addition to the migration and maintenance of Blastema cells on mesenteric decellularized scaffold showed that these scaffolds were able to restore the structure of blood capillaries, fibroblast and fat cells.Based on histological findings, the results indicate that the Blastema tissue has dynamic cells that can migrate into the scaffold.Furthermore the characteristics of the mesenteric decellularized ECM can support adhesion, migration and differentiation of blastema cells in vitro.
S.F Fani Yazdi; M Rajabian; N Mahadavi Shahri
Volume 2, Issue 4 , March 2014, , Pages 67-75
Abstract
Abstract The Saffron plant (Crocus sativus), a native plant from Iran and especially of Khorasan region, has a specific place for people's diet. According to several studies about effects of Saffron and because there are no specific studies on the effects of Saffron on the development of Drosophila melanogaster ...
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Abstract The Saffron plant (Crocus sativus), a native plant from Iran and especially of Khorasan region, has a specific place for people's diet. According to several studies about effects of Saffron and because there are no specific studies on the effects of Saffron on the development of Drosophila melanogaster as an animal model, this subject is being evaluated in the current study. A total of 5 pairs of 3-days-old wild Drosophila melanogaster were transferred to every culture plate containing different concentrations of Saffron in order to intercross and oviposition and were brought out after 8 hrs. sampling of larvae were performed in the seven steps at intervals of twelve hours and in each step, Morphometrical changes in the length and width of the larvae, were studied. The obtained data were evaluated statistically using SAS software, The variance between groups were evaluated by the test of One way ANOVA, and the mean of data were compared using Ttukey test with minimum significance level of p in high concentrations, has inverse effect on the biology of this insect.
