Biochemistry
Asieh Emami Nejad; Mohammad Fazilati; Hossein Salavati; Habiballah Nazem
Abstract
The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine ...
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The use of protease enzymes and their stabilization has been greatly expanded due to application in various industries. Alkaline serine protease enzymes are very important due to their thermal stability, activity and stability. In this study, a new approach to increase the half-life of alkaline serine protease enzymes using selenium and calcium nanoparticles was presented. Due to its high biocompatibility, toxicity, and low cost, these nanoparticles have become one of the most prominent non-metallic oxide nanoparticles, which, with their antioxidant and anti-inflammatory properties, can protect against the destruction of enzymes by free radicals. The results showed that these nanoparticles can significantly increase the half-life of these enzymes. On the other hand, the serine protease enzyme immobilized on selenium nanoparticles has unique biochemical characteristics in the presence and absence of calcium. The obtained results indicate that the stabilized enzyme has high activity and stability in a wide range of temperatures and pH compared to pure and impure-free enzyme. The results showed that the enzyme immobilized on selenium nanoparticles increased its activity by more than 99% in the presence of calcium at the temperature of 45°C. On the other hand, the half-life of the free enzyme increased from 95 minutes in the pure form to 115 minutes in the form immobilized on nanoparticles in the presence of calcium. These results indicate that the presence of nanoparticles can prevent the destruction of enzymes by free radicals.
Veterinary
Sheida Sadat Zonoori; Mohammad Fazilati; Hossein Salavati
Abstract
Today, Spirulina micro-algae is cultivated in many countries as a rich source of protein, unsaturated fatty acids, vitamins, minerals and antioxidants. In this study, the effect of replacing spirulina microalgae powder with different amounts of industrial vitamin-mineral premix was compared as an additive ...
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Today, Spirulina micro-algae is cultivated in many countries as a rich source of protein, unsaturated fatty acids, vitamins, minerals and antioxidants. In this study, the effect of replacing spirulina microalgae powder with different amounts of industrial vitamin-mineral premix was compared as an additive to stimulate the growth performance of broiler chickens. 200 one-day-old broiler chickens were randomly selected among 3000 chickens and divided into five treatments at Mehrgan Jihad Agricultural Research Center in Kermanshah province The diet of the first group, which was considered as a control treatment, includes (100% industrial supplement and no spirulina), the first treatment T1(25% spirulina and 75% industrial supplement), the second treatment T2 (50% spirulina and 50% industrial supplement), The third treatment T3 (75% spirulina and 25% industrial supplement) and the fourth treatment T4 (100% spirulina) were used. After 42 days, there was no mortality in the treatments, in the T1, T2 and T4 treatments, live weight significantly (P<0.05) increased compared to the control group, and the amount of feed conversion ratio (FCR) in the T2 treatment decreased significantly. Therefore, T2 showed the best performance Then the biochemical parameters of blood serum in T2 were checked, which showed a significant (P<0.05) increase in glucose, total protein and albumin, HDL and LDL and a decrease in triglyceride and cholestrol levels. Based on findings, it is concluded that by replacing 50% of the industrial vitamin-mineral supplement with spirulina powder, the growth performance, feed conversion ratio and blood parameters in broilers can be improved.
Leyla Najafi; Mohammad Fazilati; Hossein Salavati
Abstract
C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various ...
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C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes.
Neda Zamani; Mohamad Fazilati; Hossein Salavati; Mehrana Koohi-Dehkordi
Abstract
Nowadays, use of natural colors in food and medicine, due to toxic effects of synthetic colors is so important. Phycocyanin (PC) is an innate blue pigment with many mercantile usages in foods, cosmetics and medicines extracted from Spirulina platensis. In addition, it has been proven that phycocyanin ...
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Nowadays, use of natural colors in food and medicine, due to toxic effects of synthetic colors is so important. Phycocyanin (PC) is an innate blue pigment with many mercantile usages in foods, cosmetics and medicines extracted from Spirulina platensis. In addition, it has been proven that phycocyanin has antifungal, antioxidant and anticancer activities. The present study was undertaken to value the antifungal activity of phycocyanin which was separated from the blue green algae Spirulina platensis cultured in Iran. Phycocyanin extracted by sonication and centrifugation and purified by ammonium sulfate sedimentation and dialysis method. Purified sample was tested by UV Spectrophotometer absorption and FT-IR and antifungal activity of phycocyanin in different concentration was investigated against Candida albicans, on sabouraud dextrose (SD) agar plates, under sterile conditions. In UV Spectrophotometer, a wide peak range at 280, 615, 652 nm was gained. Also, structure and molecular bonds of phycocyanin was confirmed via FT-IR. An Anticandidal activity of phycocyanin was confirmed and maximum anti-fungal activity was observed in 20 and 25 mg/ml concentration of phycocyanin. In the present study, the use of phycocyanin has shown an antifungal effect against Candida albicans. This pigment seems to be a good alternative to chemical drugs in the treatment of Candidiasis.
