Yazdan Keivany; Sedigheh Mahmoodi; Salar Dorafshan; Mansoureh Malekian
Abstract
So far, 13 species were recently recognized in the Iranian basins. In the present study the variation among A. eichwaldii, A. namaki, A. idignensis, and A. nicolausi, A. qanati A. petrubanarescui has been investigated from morphological and molecular approaches. To study the morphological characteristic, ...
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So far, 13 species were recently recognized in the Iranian basins. In the present study the variation among A. eichwaldii, A. namaki, A. idignensis, and A. nicolausi, A. qanati A. petrubanarescui has been investigated from morphological and molecular approaches. To study the morphological characteristic, 144 specimens of these six species were used, on which 22 morphometrics were measured. Nine meristics characteristics were counted. Analysis of variance (ANOVA), discriminant analysis, and principal component analysis was performed. In the molecular studies, sequences of the mitochondrial gene, cytochrome b from six species were sequenced and analyzed. The morphological results of the data showed a high similarity among species, but could separate A. nicolausi and A. idignensis according to the PC1. Also A. idignensis and A. petrubanarescui are more similar based on the PC2. Moreover, based on meristic characteristics which were relatively similar, again A. nicolausi and A. idignensis could be separated by the first component. The phylogenetic analysis using Maximum Likelihood (ML) and Maximum-Parsimony (MP) methods supported the monophyletic status of the six species, suggesting their status as different species. The results of the molecular analysis showed that A. eichwaldii and A. petrubanarescui have the most similarity (about 99%) and the A. idignensis has the maximum difference (about 85%) with the other species. These results justify the results of morphometric analysis.
E. Haghighy; M. Sattari; S. Dorafshan; Y. Keivany
Volume 3, Issue 4 , July 2015, , Pages 37-46
Abstract
Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters ...
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Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters were measured and thirteen meristictraits were counted on each specimen. Nonparametric methods for comparison of means and Principal Component Analysis (PCA) for separation of populations were used. Among the meristic characters, nine characters were significantly different between the four populations (p<0.05). Among the morphometric and relative morphometric characters, nineteen characters showed significant differences (p<0.05) between the two populations. In the PCA of meristic characters, three factors accounted for 47.5% of variations within populations, including; caudal peduncle scales, scale above lateral line and anal fin ray numbers. Within the morphometric characters, three factors accounted for about 71% of variations within the two populations, including dorsal fin height, pectoral fin base, ventral fin length, caudal peduncle depth, head length, inter orbital width, standard length to pre dorsal length, head length to snout length, head length to eye diameter and head length to inter orbital width. The results showed while morphometric and relative morphometric characters were not useful for separating the two populations and sexes, the meristic characters could relatively separate these two populations.
