Animal physiology
ramesh monagemi; noushin naghsh
Articles in Press, Accepted Manuscript, Available Online from 19 October 2014
Abstract
Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian ...
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Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian C. medica on Hela cell line. After preparation extract and volatile oils, different concentrations of volatile oils of C. medica (0.25-10 μg / ml) and extract of Citrullus Colocynthis (100-1000 μg / ml) were added to Hela cultured cells and incubated for 72 hours. Cell survival was evaluated using MTT-based cytotoxicity assay. The results showed that methanolic extract of Citrullus Colocynthis had not significant cytotoxic effect on Hela cells but Volatile oils of Citrus medica had significant cytotoxic effect on this cells and it’s most compounds was limonene , linalool , linalool acetate and beta - pinene. The findings showed that volatile oils of C. medica had more cytotoxic effect than the Citrullus Colocynthis extract, limonene and linalool of the volatile oil causes this effects.
Kahin Shahanipoor; Sara Farhadbakhtiarian; Ramesh Monajemi
Volume 4, Issue 4 , May 2016, , Pages 27-33
Abstract
Abstract Prostate cancer is the second most common cancer in men after skin cancer. Throughout the years, medicinal plants, in some cases is the only treatment were considered. Glycyrihiza glabra L. liquorice plant with the scientific name 'Glycyrihiza glabra L.' which belongs to the family Fabaceae, ...
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Abstract Prostate cancer is the second most common cancer in men after skin cancer. Throughout the years, medicinal plants, in some cases is the only treatment were considered. Glycyrihiza glabra L. liquorice plant with the scientific name 'Glycyrihiza glabra L.' which belongs to the family Fabaceae, there are three species of perennial herbaceous plants in Iran. Root extract is widely used in medicine, food industry, Tobacco and other industries throughout the world. In this study, the cytotoxic effect of aqueous and hydroalcoholic extracts of licorice root on human prostate cancer (DU145) with the MTT assay was studied. Licorice root through Agriculture and Natural Resources Research Center of Isfahan was prepared. Next the aqueous and hydroalcoholic (ethanol) extracts of the plant was prepared. The cell category of DU-145 in the Cultivation enviroment RPMI-1640 Containing 10% bovine serum in incubator were cultured with 5% CO2 and in different concentrations of the aqueous and Hydroalcoholic (ethanol) during 24, 48, 72 hours was incubated. The method of MTT for calculating the survival rate of the cells were incubated in the presence and the Lack extracts were used and Light absorption at 540 nm was read by ELISA. Data were evaluated using SPSS software (N=12, *=P-value < 0.05). The results show a decrease in the survival rate of the cells was determined by MTT assay. The results of this study, it states that aqueous extract and hydroalcoholic extracts of licorice plant may have some cytotoxic effects. Because of terpene compounds in licorice.
R. Monajemi; N. Naghsh; M. Kameli
Volume 2, Issue 2 , September 2014, , Pages 45-51
Abstract
Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian ...
Read More
Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was investigate the cytotoxic effects of the methanol extract of Citrullus Colocynthis and volatile oils of Iranian C. medica on Hela cell line. After preparation extract and volatile oils, different concentrations of volatile oils of C. medica (0.25-10 μg / ml) and extract of Citrullus Colocynthis (100-1000 μg / ml) were added to Hela cultured cells and incubated for 72 hours. Cell survival was evaluated using MTT-based cytotoxicity assay. The results showed that methanolic extract of Citrullus Colocynthis had not significant cytotoxic effect on Hela cells but Volatile oils of Citrus medica had significant cytotoxic effect on this cells and it’s most compounds was limonene , linalool , linalool acetate and beta - pinene. The findings showed that volatile oils of C. medica had more cytotoxic effect than the Citrullus Colocynthis extract, limonene and linalool of the volatile oil causes this effects.
L. D alali Isfahani; R. Monajemi; L. Amjad
Volume 1, Issue 3 , November 2012, , Pages 1-6
