Fisheries
Bita Seraj; Mesbah Mehrzad; Paria Akbari
Abstract
In this study, in addition to the LC50 and the maximum allowable concentration, effects of silver nanoparticles on several of immune and blood serum enzymes parameters of Barbus grypus were determined For this purpose, 240 shirbut, to investigate the effects of sub lethal toxicity were divided as follows-control ...
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In this study, in addition to the LC50 and the maximum allowable concentration, effects of silver nanoparticles on several of immune and blood serum enzymes parameters of Barbus grypus were determined For this purpose, 240 shirbut, to investigate the effects of sub lethal toxicity were divided as follows-control treatment (G1), the concentration LC50 1% (0.012 mg/L AgNP) (G2), concentration LC50 2% (0.025 mg/L AgNP) (G3) and concentration LC50 4% (0.05 MG/L AgNP) (G4) and concentration LC50 8% (0.1 Mg/ L AgNP) (G5). Then on days zero, 7, 14 and 21 immune responses and serum enzymes (ALT, LDH, SGOP, SGPT) in Barbus grypus were measured. In the study, WBC counts increased significantly (P<0.05) in G2, G3, G4 and G5 treatments compared to control (G1). Serum anti-bacterial activity was enhanced in G3 in twenty first day and G4 and G5 in 14th and 21s days in compared to control. Serum lysozyme activity enhanced significantly (P<0.05) in (G4) in 14th and (G5) 14th and 21s in days compared to control. ALT and LDH Level decreased significantly in (G2), (G3), (G4) and (G5) treatments in 21s day compared to control. SGPT level decreased significantly in (G2), (G3), (G4) and (G5) in 21s day compared to control. SGOT level decreased significantly in (G3), (G4) and (G5) treatments in 21s day compared to control. According to the results of this study concentrations toxicity of nano silver increased immune responses and serum enzymes.