B. Taneh; B. Abtahi; R.M. Nazari
Volume 1, Issue 1 , October 2012, , Pages 31-40
Abstract
One of the big problems on Sturgeon Fishes is select of the breeders that have high quality of gametes after hormone injection. So in this research Final oocyte maturation index (GVBD) in in vitro for selection of Persian sturgeon Broodstock, Acipenser persicus, was studied on 10 females ...
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One of the big problems on Sturgeon Fishes is select of the breeders that have high quality of gametes after hormone injection. So in this research Final oocyte maturation index (GVBD) in in vitro for selection of Persian sturgeon Broodstock, Acipenser persicus, was studied on 10 females captured from the Caspian Sea during reproduction season. About 150 oocytes from every broodstock, taken by hollow probe, were incubated in incubation media of RM2 containing 10 µg/l of progesterone for induction of the final oocyte maturation and assessment of GVBD index. Results indicated that significant differences in GVBD of the in vitro and in vivo were observed (٭٭). However, Broodstockes with rate of GVBD in in vitro higher than %50 had a significantly better ovulation, fertilization and incubation rates (٭), from those with a GVBD lower than %50. It could be concluded that use from method of assessment of GVBD index under in vitro circumstance was preferable to assessment of GVBD index under in vivo and also nucleus polarization index for selection of suitable broodstock.