esmat salimi; Hosein Zolgharnine; Bita Archangi
Volume 6, Issue 4 , June 2018, , Pages 35-40
Abstract
Abstract Iran with a vast geographical distribution has several habitats and a high ecological importance. Among the ecological studies done a few studies have been conducted on Iran's internal waters. Knowing the biological and ecological characteristics of different aquatic species in Iran can provide ...
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Abstract Iran with a vast geographical distribution has several habitats and a high ecological importance. Among the ecological studies done a few studies have been conducted on Iran's internal waters. Knowing the biological and ecological characteristics of different aquatic species in Iran can provide useful information for effective fish stock management in future. Aphanius ginaonis is an endangered species and have high ecological importance. In this study, Genetic diversity of Aphanius ginaonis was studied through PCR-RFLP method through mitochondrial DNA in D-loop region. To conduct this study, 30 Aphanius ginaonis from Geno hot spring in Hormozgan taken. The samples'DNA was extracted through phenol chloroform method. The length of PCR product was 550 bp. To digest PCR product, 5 restriction enzymes (AluI, DpnI, Eco47I, HindIII,HinfI) have been used. The data was analysed through Arlequin 3.11 software. 9 different haplotypes were revealed through analysis which four haplotypes were rare haplotype. Haplotypes EABAB, EACAB and CABAB showed the highest frequency. High haplotype and nucleotide diversity is resulted in this species. Results obtained from this research would be applicable to understand conservation genetics and management of this important fish species in Iran.
