esmat salimi; Hosein Zolgharnine; Bita Archangi
Volume 6, Issue 4 , June 2018, , Pages 35-40
Abstract
Abstract Iran with a vast geographical distribution has several habitats and a high ecological importance. Among the ecological studies done a few studies have been conducted on Iran's internal waters. Knowing the biological and ecological characteristics of different aquatic species in Iran can provide ...
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Abstract Iran with a vast geographical distribution has several habitats and a high ecological importance. Among the ecological studies done a few studies have been conducted on Iran's internal waters. Knowing the biological and ecological characteristics of different aquatic species in Iran can provide useful information for effective fish stock management in future. Aphanius ginaonis is an endangered species and have high ecological importance. In this study, Genetic diversity of Aphanius ginaonis was studied through PCR-RFLP method through mitochondrial DNA in D-loop region. To conduct this study, 30 Aphanius ginaonis from Geno hot spring in Hormozgan taken. The samples'DNA was extracted through phenol chloroform method. The length of PCR product was 550 bp. To digest PCR product, 5 restriction enzymes (AluI, DpnI, Eco47I, HindIII,HinfI) have been used. The data was analysed through Arlequin 3.11 software. 9 different haplotypes were revealed through analysis which four haplotypes were rare haplotype. Haplotypes EABAB, EACAB and CABAB showed the highest frequency. High haplotype and nucleotide diversity is resulted in this species. Results obtained from this research would be applicable to understand conservation genetics and management of this important fish species in Iran.
Halimeh Rajabi; Hossein Zolgharnen; Mohammad Taghi Ronagh; Ahmad Savari; Mohammad Sharif Ranjbar
Volume 4, Issue 4 , May 2016, , Pages 19-25
Abstract
Abstract Genomic DNA extraction with high quantity and quality is the basic necessities of molecular genetic studies. The aim of this study is the applicability of different DNA extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, ...
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Abstract Genomic DNA extraction with high quantity and quality is the basic necessities of molecular genetic studies. The aim of this study is the applicability of different DNA extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snail Conus coronatus, Mollusca with Phenol-chloroform, Ammonium acetate and CTAB (Cetyl trimethylammonium bromide) methods. About 30 samples were collected from Qeshm Island, Zeyton Park. After dissection on sterile condition, different kinds of Conus tissues were separated and preserved in ethanol 96%, then transferred to the lab. DNA extraction were performed from different tissues with different methods. Spectrophotometer and electrophoresis on 1 % agarose gel were used for evaluating the quality and quantity of extracted DNA samples. According to the results, Ammonium acetate method showed sharper bands, without esmire in comparison with other extraction methods and DNA extracted from foot tissue had the best quality and quantity.
