Behzad Shareghi; Maryam Kazemi Nafchi
Volume 6, Issue 4 , June 2018, , Pages 23-33
Abstract
Abstract Peroxidases are a group of oxidoreductases that are produced by a number of microorganisms and plants, and catalyse the reduction of peroxides. Peroxidases are widely used in clinical biochemistry and enzyme immunoassay. Horseradish peroxidase isoenzyme C (HRPC) is one of the characterized peroxidases. ...
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Abstract Peroxidases are a group of oxidoreductases that are produced by a number of microorganisms and plants, and catalyse the reduction of peroxides. Peroxidases are widely used in clinical biochemistry and enzyme immunoassay. Horseradish peroxidase isoenzyme C (HRPC) is one of the characterized peroxidases. The structure of the enzyme is largely alpha helical. Peroxidase enzyme is detox important enzyme that to work process for getting rid of the cells of additional hydrogen peroxide under normal and stress conditions, including contamination with toxic levels of heavy metals, however, severe stress possible influence upon the activity of detoxification enzyme. Kinetics studies of peroxidase enzyme were performed using a spectrophotometer UV-Vis fitted with electronic control system at 35 °C and 45 °C and pH4 and in the presence ferric oxide and copper oxide. Kinetic parameters show that ferric oxide and copper oxide becomes caused the decrease of maximum speed (VMAX) and activity of the enzyme. Likely due to the ferri and copper ions have positively charged, these places are on or near the sites of glycosylation of enzyme and by their negatively charged are suitable binding sites for iron and copper. Likely binding of iron and copper are caused more changes of the secondary structure and makes random coil deduction more than alpha helix.
Behzad Shareghi; Maryam Kazemi Nafchi
Volume 5, Issue 1 , September 2016, , Pages 1-8
Abstract
Abstract Peroxidases are a group of oxidoreductases that are produced by a number of microorganisms and plants, and catalyse the reduction of peroxides. Peroxidases are widely used in clinical biochemistry and enzyme immunoassay. Horseradish peroxidase isoenzyme C (HRP) is one of the characterized peroxidases. ...
Read More
Abstract Peroxidases are a group of oxidoreductases that are produced by a number of microorganisms and plants, and catalyse the reduction of peroxides. Peroxidases are widely used in clinical biochemistry and enzyme immunoassay. Horseradish peroxidase isoenzyme C (HRP) is one of the characterized peroxidases. The structure of the enzyme is largely alpha helical. Kinetics studies of peroxidase enzyme were performed using a spectrophotometer UV-Vis fitted with electronic control system at 35oC and 45oC and pH4 and in the presence ethanol and butanediol. Kinetic parameters show that ethanol and butanediol organic solvents becomes caused the increase of maximum speed (VMAX) and activity of the enzyme. Organic solvents have effect on electrostatic interactions of proteins because their dielectric constant with water is different. In general, reduce properties of polar solvents and decreased dielectric constant becomes caused the increases electrostatic repulsion, leading to the opening of the proteins. Enzymes activity in such organic environments increases stability, activity or facilitate reactions that are difficult to perform in aquatic environments.
