Biochemistry
Asiye Seyyed; Seyyede Omolbanin Ghasemian
Abstract
Diabetes is one of the most common chronic diseases with a high prevalence that increases with age. It is predicted that by 2030, more than 360 million people in the world will have diabetes. The present study aimed to investigate the effect of intermittent and continuous training on leptin receptor ...
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Diabetes is one of the most common chronic diseases with a high prevalence that increases with age. It is predicted that by 2030, more than 360 million people in the world will have diabetes. The present study aimed to investigate the effect of intermittent and continuous training on leptin receptor expression in brain tissue and food intake in aged rats. This basic and experimental research was conducted on 32 female Sprague-Dawley rats. The rats were randomly divided into four groups: healthy control, diabetic control, intermittent training, and continuous training, with each group consisting of 8 samples. The training protocols involved were tailored to test the specific impact of different exercise regimens. The intermittent training group underwent a regime of high-intensity interval training, while the continuous training group engaged in steady-state, moderate-intensity exercise. The control groups did not participate in any structured physical activity. Following the training period, leptin levels and food intake were meticulously measured. Leptin gene expression in the brain tissue was assessed using Real-Time PCR, a highly sensitive and specific method for quantifying gene expression. Food intake was monitored and recorded at the beginning and end of the study period. The results demonstrated a statistically significant increase in leptin gene expression in the brain tissue of the rats subjected to intermittent training (P=0.001). This suggests that intermittent training may more effectively stimulate molecular pathways associated with leptin receptor expression compared to continuous training. Furthermore, a significant difference in food intake was observed between the groups after the eight-week training period (P=0.001). Tukey’s post hoc analysis revealed a significant difference between the high-intensity interval training and continuous training groups in terms of leptin expression (P=0.03), indicating that the type and intensity of training can differentially influence leptin receptor dynamics. Additionally, food intake in the diabetic control group was significantly higher compared to the training groups (P=0.001), suggesting that physical activity can mitigate hyperphagia in diabetic conditions. However, both interval and continuous training exerted a similar effect on overall food intake (P=0.58), implying that while the type of exercise influences leptin expression, the regulation of food consumption might be governed by other compensatory mechanisms. In conclusion, the findings underscore the significant impact of intermittent training on leptin gene expression in brain tissue of aged rats, highlighting its potential advantages over continuous training in modulating molecular markers linked to energy homeostasis. Nonetheless, both training modalities exhibited comparable effects on food intake, emphasizing the complexity of exercise-induced metabolic regulation.
Mansour Farajollahi Moghadam; Omolbanin Ghasemian
Abstract
Diabetes mellitus is one of the most serious global health problems, and its occurrence increases with age. Considering the relationship between aging and metabolic disorders, it seems that each year a large number of elderly people die due to cardiovascular diseases caused by these factors. The present ...
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Diabetes mellitus is one of the most serious global health problems, and its occurrence increases with age. Considering the relationship between aging and metabolic disorders, it seems that each year a large number of elderly people die due to cardiovascular diseases caused by these factors. The present study aimed to investigate the effect of four weeks of intense interval training along with the consumption of tryptophan supplementation on cardiac damage index in elderly diabetic rats. In this experimental study, 40 diabetic elderly rats were randomly divided into four groups, each group having 10 rats. These were control, sham, high-intensity interval training (HIIT), and HIIT+tryptophan supplementation (at a dose of 50 mg/kg) groups. The levels of lactate dehydrogenase (LDH), aspartate transaminase (AST), and creatine kinase-MB (CK-MB) was measured in each group. The results showed that the levels of LDH, AST, and CK-MB in the diabetic control group were significantly higher compared to the healthy control group (P= 0.05). Moreover, the LDH level in the HIIT + tryptophan group was significantly lower than that in the diabetic control group (P= 0.03). AST levels in the tryptophan, HIIT (P=0.001), and HIIT+tryptophan groups (P=0.001) were significantly lower compared to the diabetic control group (P= 0.001). Based on the obtained results, the indicators of heart disease improved with exercise and consumption of tryptophan. However, further studies are recommended to investigate the effects of intensity and type of training as well as the dosage of tryptophan in this regard.
Biochemistry
Asiye Seyyed; Fatemeh Harfsheno; Maryam Khorasani nejad; omolbanin ghasemian; Hassan Johari
Abstract
Statement of the problem: The liver is the main organ responsible for metabolic control and metabolic detoxification, which significantly contributes to the clearance of beta-amyloid plaques. Aim: The present study aimed to investigate the effect of aerobic exercise with a supplement of vitamin ...
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Statement of the problem: The liver is the main organ responsible for metabolic control and metabolic detoxification, which significantly contributes to the clearance of beta-amyloid plaques. Aim: The present study aimed to investigate the effect of aerobic exercise with a supplement of vitamin C on liver enzymes and total antioxidant capacity (TAC) values in rats with Alzheimer's disease.Materials and Methods: This was an experimental and fundamental research with a post-test and a control group conducted on 35 aged male Sprague-Dawley rats. The samples were randomly assigned to five groups (7 cases in each group), including 1) Alzheimer, 2) the healthy, 3) the exercise group, 4) the vitamin C, and 5) the exercise+vitamin C group. Aerobic exercise was continued for eight weeks, Total protein in liver tissue was measured to assess TAC, Malondialdehyde (MDA, alanine aminotransferase (AST), alkane phosphatase (AST), and aspartate aminotransferase (AST) levels.Results: The values of AST, ALT and ALP, MDA, and TAC in the control group with Alzheimer's disease were significantly higher than those in the healthy control group (P<0.05). After the intervention, AST, ALT, and ALP in the exercise and vitamin C supplement groups were significantly lower than in the non-exercise groups (P=0.001).Conclusion: Aerobic exercise and vitamin C supplementation are effective in improving liver factors in rats with Alzheimer's disease; however, they have no therapeutic effect on MDA and TAC levels in rats with Alzheimer's.
