Development
Nasim Hayati; Amirhosein Fazlali; Gholamreza Kaka; Kazem Parivar
Abstract
Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were ...
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Objective background: This study evaluated the therapeutic effect of umbilical cord blood cells on PLGA membrane scaffold on the repair of severed sciatic nerve of male Wistar rats by behavioral and electrophysiological studies.
Materials and Methods: After cutting the sciatic nerve, male rats were divided into 4 groups of 7 Healthy rats, sciatic nerve cut rats without therapeutic intervention, rats with umbilical cord blood cells on PLGA membrane scaffold at the nerve cut site, rats were treated with umbilical cord blood cell injection at the injury site. The degree of recovery was evaluated by the sensorimotor activity of the sciatic nerve, electrophysiological studies.
Findings: motor evaluation of the sciatic nerve, the control group was not observed to return to the normal state in the eighth week, the cell therapy group was repaired on the PLGA membrane scaffold in the eighth week. The level of AMP in the 8th week after recovery of the cell therapy group with a gentle slope is a sign of the healing process of the cell therapy group. Counting the number of nerve fibers in a surface equal to 1000 square meters, the number of nerve fibers in the cell therapy groups increased after the eighth week of repair, compared to the control group and the PLGA membrane group. At the end of the eighth week, the sensory activity index of the sciatic nerve (Hot Plate test), the healing process of the cell therapy group on the PLGA membrane scaffold was more evident than the other groups.
Conclusion: Umbilical cord blood cell transplantation causes sciatic nerve repair and PLGA membrane scaffold with cord blood cells accelerates sciatic nerve repair.
