genetics
Hanieyh Heydarzadeh; Vida Hojati; Farid Ebnerasuly
Abstract
Blood fat is a global and increasing problem in all countries, including Iran, and one of the most important threats to society's health. High blood fat as a multifactorial problem is under the influence of various factors, especially metabolic and genetic factors. The aim of this study is to investigate ...
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Blood fat is a global and increasing problem in all countries, including Iran, and one of the most important threats to society's health. High blood fat as a multifactorial problem is under the influence of various factors, especially metabolic and genetic factors. The aim of this study is to investigate the rs2016520 polymorphism in the PPARD gene and its relationship with the level of low-density lipoprotein (LDL). Blood sampling from 137 people, including 83 Iranian women and 54 men, including 79 healthy people or controls (normal LDL) (57.7%) and 58 people with LDL above 130 (42.3%) in the age range of 18 to 60 years. It was done after obtaining consent. DNA was extracted by a ready kit. The concentration and quality of isolated DNA were measured by spectrophotometry and agarose gel. Genotypes were identified by PCR-RFLP method. 6% frequency of genotypes in the group with normal LDL: 3.8% for CC, 36.7% for CT and 59.5% for TT genotype and in the group with LDL higher than 130: 12.1% for CC, 37.9% for CT and it was 0.50% for TT genotype. The difference in the frequency of genotypes between the two groups with normal LDL and the group with LDL above 130 was not significant (p > 0.05). 2.56% of the samples had a BMI lower than 25 and 43.8% had a BMI higher than 25. The results of the present study did not show a significant relationship between rs2016520 polymorphism and LDL level.
