Microbiology
Reza Yadi; Fatemeh Haghshenas Gatabi; Naeimeh Dehghani; Hamed Daneshpazhouh
Abstract
Introduction: Due to increasing resistance of pathogenetic bacteria to the new antibiotics, the researchers are seeking of finding the antimicrobial materials with herbal origins as the replacement of ineffective antibiotics. The aim of doing of this study is evalution of the antibacterial effect of ...
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Introduction: Due to increasing resistance of pathogenetic bacteria to the new antibiotics, the researchers are seeking of finding the antimicrobial materials with herbal origins as the replacement of ineffective antibiotics. The aim of doing of this study is evalution of the antibacterial effect of hydro alcoholic extraction of the plant of thymus daensis on the bacteria of shigella flexneri, Yersinia enterocolitica and streptococcus salivarius.
Investigation method: in this practical study, the plant of thymus daensis were collected and the extraction of plant were prepared by the method of maceration and the antimicrobial effects of these plant in the amounts of 0.65, 1.25, 2.5, 5 and 10 mg/l were prepared by using the method of transmission on disk on the bacteria of shigella flexneri, Yersinia enterocolitica and Streptococcus salivarius compared with the antibiotics of Penicillin, Gentamicin, Tetracycline and Vancomycin and drop of halo of lack of growth was measured. Also the minimum concentration of restraining of growth (MIC) and the minimum concentration of lethalithy (MBC) of extractions against three concerned bacteria were determined.
Results: The obtained results showed that the extraction of thymus daensis on streptococcus salivarius in the concentration of 10mg/l had the most effect and the drop of halo of lack of growth were respectively measured as 20.83, 20.33, 19. Also this extractions didn’t have any effect on the bacterium of Yersinia for mutuol effect of antibiotic in bacterium the maximum drop of halo of lack of growth for antibiotic of Tetracycline and bacterium of Streptococcus salivarius was achived but in the mutual effect of antibiotic of Vancomycin and the bacterium of Yersinia any halo wasn’t composed. The amount of mic related to thymus daensis on the bacteria of Streptococcus salivarius and Shigella flexneri showed a similar amount the equals to 0.65 mg/l and the amount of mic related to thymus daensis on the bacterium of Yersinia enterocolitica was achieved equals to1.25 mg/l. the amount of mbc component for the plant of Thymus daensis on the bacteria of Yersinia and Shigella was achived equals to 2.5 mg/l and for bacteria of Streptococcus salivarius was achived equals to 1.25 mg/l .
Conclusion: There for, the hydro-alcoholic extraction of thymus daensis has an inhibitory effect, this extractions showed more effect on the bacterium of Streptococcus than two bacteria Yersinia and Shigella.
Animal physiology
Hamed Daneshpazhouh; Nasim Hayati Roodbari; Mehdi Dianatpour; Yaser Tahamtani; zahra khodabandeh
Abstract
Freezing is a long-term egg storage method that plays an important role in assisted reproductive methods. The aim of the present study is to investigate the effect of freezing solutions and docetaxel on the expression changes of autophagy genes such as Atg5 and Beclin-1 in mouse MII oocytes after glass ...
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Freezing is a long-term egg storage method that plays an important role in assisted reproductive methods. The aim of the present study is to investigate the effect of freezing solutions and docetaxel on the expression changes of autophagy genes such as Atg5 and Beclin-1 in mouse MII oocytes after glass freezing by cryotop method. To achieve this goal, mouse MII oocytes were collected and frozen in two different concentrations of 15% ethylene glycol, 15% dimethyl sulfoxide and 0.5 M sucrose in group A (VS1) and 7.5% ethylene glycol, glycerol. 7.5% and 0.5 M sucrose were frozen in group B (VS2) and some groups were affected by docetaxel before freezing. After thawing, the eggs were fertilized.The percentage of survival and fertilization of frozen and thawed oocytes was evaluated and the expression changes of genes (Atg5 and Beclin-1) were investigated by RT-PCR method. The results showed that there are significant differences between the percentage of survival and the percentage of fertilization in the freezing groups compared to the control group (P<0.05). The percentage of survival and fertilization in the VS1 group decreased compared to the VS2 group. Also, the percentage of survival and conception of the groups pre-incubated with Docetaxel was higher than the non-incubated groups. This study showed that vitrification with cryotop changes the transcript levels of autophagy genes in frozen-thawed MII oocytes, and pre-incubation of oocytes with docetaxel before vitrification can decrease the transcript levels of Atg5 and Beclin-1 in the experimental groups and above Increase the percentage of survival and the percentage of formation of two-celled embryos.
