Animal physiology
Fatemeh Yarifar; Zahra Baghaeifar; Shahriar Saeedian; Hamid Darvishnia
Abstract
The most widely used colon cancer screening test is the diagnostic test for checking the presence of blood in stool. This test is utilized to examine the occult blood sample present in the stool or the blood compounds that may arise in the event of minor bleeding within the digestive tract. In this study, ...
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The most widely used colon cancer screening test is the diagnostic test for checking the presence of blood in stool. This test is utilized to examine the occult blood sample present in the stool or the blood compounds that may arise in the event of minor bleeding within the digestive tract. In this study, the serum amylase levels of OB-positive patients (with occult blood in the stool) were examined as a predictor of gastrointestinal malignancies. Ninety individuals aged 50 and over who referred to the laboratory department of Tabriz Alinasab Hospital were divided into three groups: healthy control, OB positive patients, and patients with colorectal cancer. The measurement of serum amylase was performed using the enzymatic calorimetric (photometric) method, using the Man company diagnostic kits and the BS-800M autoanalyzer. Data analysis was performed using SPSS software, and the ANOVA test was used to show the relationship between variables. The healthy group did not exhibit a significantly different serum amylase level from that of OB-positive patients who didn’t exhibit colorectal cancer symptoms. The amount of serum amylase in patients with colorectal cancer increased significantly compared to the healthy group (p<0.05). In patients with colorectal cancer, a significant increase in serum amylase levels was observed compared to OB positive patients without colorectal cancer symptoms (p<0.05). It’s possible to conclude that a positive occult blood doesn’t always indicate cancer. Positive occult blood in the stool increases the risk of developing various diseases. Therefore, other patients with occult blood in the stool should be given a colonoscopy more seriously.
Shahriar Saeidian; Zahra Baghaei Far; Zivar Parvanak; Mokhtar Fathi
Abstract
Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential ...
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Breast cancer accounts for about 10% of all cancers in the world and accounts for 30% of all cancers in women. Therefore, its early detection will play an important role in its treatment. Because lncRNAs are expressed differently in cancerous tissues than in normal tissues, they increase the potential of these molecules as biomarkers for disease diagnosis. Also, changes in the expression of lncRNAs in patients with different types of cancer subtypes and different races intensify the importance of using these molecules as biomarkers for disease diagnosis. Therefore, the aim of this study was to investigate the expression of GAS5, NEAT1 and SRA lncRNAs in cancer specimens with cancer and in healthy individuals. In this study, from the tumor tissue of 22 patients with breast cancer and also 22 samples of healthy tissue from individuals under the direct supervision of a pathologist and according to clinical signs and laboratory findings were collected from hospitals in Isfahan. After RNA extraction from tumor and normal tissue, cDNA was fabricated according to RT-qPCR method. The lncRNA expression level of GAS5, NEAT1 and SRA genes was calculated by ∆∆CT method. The expression pattern was analyzed using Rest 2009 software as well as SPSS version 16. Real Time Reverse transcription-PCR results showed that the mean relative gene expression in tumor samples was significantly lower for GAS5 and NEAT1 lncRNAs than normal samples. But no expression change was observed for lncRNA SRA.
