Veterinary
Parvaneh Pakzad; Saleh Tabatabaei Vakili; Morteza Mamouei; Jamal Fayazi
Abstract
The present study was performed to investigate the effect of injection of PMSG and GnRH hormones on reproductive performance of Arabi ewes in the breeding season. 75 ewes were divided into 5 groups. The first group had no estrus synchronization program and no hormonal injection (control). Vaginal progesterone ...
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The present study was performed to investigate the effect of injection of PMSG and GnRH hormones on reproductive performance of Arabi ewes in the breeding season. 75 ewes were divided into 5 groups. The first group had no estrus synchronization program and no hormonal injection (control). Vaginal progesterone sponge (MAP) was inserted into other groups for 14 days. Simultaneously with the withdrawal of sponge, they were treated with: 1- injection of 500 IU of PMSG, 2- injection of 100 micrograms of GnRH, 3- injection of 500 IU of PMSG plus 100 micrograms of GnRH and 4- without injection (MAP only). One healthy and fertile Arabi ram was assigned to each of the 5 ewes. Estrous behavior of ewes was recorded for 7 days. The estrous response, return to estrus, pregnancy length, lambing rate, prolificacy rate, fecundity rate and the ratio of the birth of male and female lambs, were not affected by treatments. Injection of PMSG and GnRH were able to reduce the time of estrus onset coMAPred to sponge treatment alone (P<0.05). The birth weight and weight of 30, 60 and 90 days in lambs, although were affected by the treatments (P<0.05), but no obvious effect of hormone injection was observed on these parameters. In general, the injection of PMSG and GnRH in the estrus synchronization program of Arabi ewes, although was an effective method in estrus onset time, but could not have a significant effect on the other reproductive performances of the animal during the breeding season.
Fatemeh Kamali; Saleh Tabatabaei Vakili; Ali Aghaei; Shima Hosseinifar
Abstract
This study was performed to evaluate the effects of hypothyroidism and hyperthyroidism on performance, blood and reproductive parameters in Japanese quail. 320 one-day-old quail chicks were used in a completely randomized design with 4 treatments and 4 replications. Treatments were included 1- untreated ...
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This study was performed to evaluate the effects of hypothyroidism and hyperthyroidism on performance, blood and reproductive parameters in Japanese quail. 320 one-day-old quail chicks were used in a completely randomized design with 4 treatments and 4 replications. Treatments were included 1- untreated (control), 2- levothyroxine (70 μg / l), 3- propylthiouracil (15 mg / l) and 4- propylthiouracil first and then levothyroxine. Performance parameters were recorded weekly. On day 42 of rearing, one male and one female were selected from each replicate and blood samples were taken to evaluate blood parameters. On day 62 of rearing, the bird's eggs were collected and transferred to the incubator. Food conversion ratio, feed intake and weight gain were not affected by the treatments. In male blood serum, triglyceride concentration in all therapeutic treatments and alanine aminotransferase concentration in propylthiouracil-levothyroxine treatment significantly reduced (P≤0.05). In female birds, blood LDL concentration decreased in levothyroxine treatment compared to control and alanine aminotransferase increased in this treatment compared to other groups (P≤0.05). The highest serum alkaline phosphatase concentration of females was observed in propylthiouracil treatment (P≤0.05). Other blood biochemical parameters and fertility percentage were not affected by the treatments. The lowest percentage of total hatching and hatching of fertile eggs and the highest percentage of embryonic mortality were related to the treatment of propylthiouracil-levothyroxine (P≤0.05). In conclusion, induction of hyperthyroidism and hypothyroidism with drugs did not have a significant effect on most functional, reproductive and blood parameters in Japanese quail.
Azadeh Tabaei; Morteza Mamouei; Saleh Tabatabaei Vakili; Khalil Mirzadeh
Abstract
The aim of present study was to evaluation the effect of different levels of soybean lecithin in tris extender replaced with egg yolk on semen quality parameters of Arabic ram after freeze-thawing process. For this purpose, the experiment with numbers of 8 adult and healthy Arabic rams with an average ...
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The aim of present study was to evaluation the effect of different levels of soybean lecithin in tris extender replaced with egg yolk on semen quality parameters of Arabic ram after freeze-thawing process. For this purpose, the experiment with numbers of 8 adult and healthy Arabic rams with an average weight of 75 ± 5 kg were used in a completely randomized design. Semen samples were collected using electro ejaculator. Semen samples were mixed and after dilution, were divided in to 5 experimental groups. Treatments were increasing levels of soybean lecithin (0.5, 1, 1.5 and 2 percent) and egg yolk (15 percent) in tris base extender and 5 percent of glycerol. The 5 numbers of 0.5 ml straws from each treatment were filled. The straws were equivalence for 2 hours in 5˚C and then freezed. After 2 weeks, straws were thawed at 37˚C. Qualitative parameters of spermatozoa including the motility, viability, morphological abnormalities, membrane integrity and the pH of semen were evaluated. The resulted of this experiment showed that extender containing 1.5 percent of soybean lecithin significantly improved total motility and progressive motility, viability, morphological abnormalities and membrane integrity of Arabic ram spermatozoa compared to the other levels of lecithin as well as egg yolk (P<0.05). The most improvement have been observed at the 1.5 % soybean lecithin. Therefore, this level can be used in tris extender for maintaining of Arabic ram spermatozoa in freeze condition.
Shadan Golandam; Saleh Tabatabaei Vakili; Khalil Mirzadeh
Abstract
The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint ...
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The aim of this study was to investigate the effect of different levels of peppermint extract on semen quality of Arabi rams. Semen was collected from 12 Arabi rams weekly for 8 weeks and immediately mixed, and then were divided into 5 parts and received zero, 50, 100, 150 and 200 μl/ml of peppermint extract. At different storage times of diluted semen containing treatments (zero, 24, 48 and 72 hours) in liquid condition, semen quality parameters were evaluated. Immediately after sperm collection, the lowest total motility and sperm viability belonged to the level of 200 peppermint extract (P<0.05). At 24 hours, levels 150 and 200 of extract increased the rate of morphological abnormalities of sperms. At this time, the lowest level of sperm plasma membrane integrity was related to the level of 200 peppermint extracts (P<0.05). 48 hours after sperm storage, the highest total motility, viability and plasma membrane integrity of sperms were belonged to the control group (P<0.05). 72 hours after semen storage, no statistically significant differences were found among treatments for all sperm quality parameters. At this time, the highest concentration of malondialdehyde (MDA) in seminal plasma was related to the level of 100 μl/ml peppermint extract (P<0.05). In general, by adding different levels of peppermint extract to the diluent and maintaining the diluted semen of the Arabi ram in a liquid state at 5°C, the concentrations used of peppermint extract not only did not improve the qualitative parameters of the sperm, it also had a devastating effect.
