Kinetic investigations of peroxidase in roots of
Gundelia Tournefortii
Shahriar
Saeidian
Assistant Professor of Biochemistry, Department of Biology, Payame Noor University, Tehran, Iran
author
text
article
2016
per
Kinetic properties of root of peroxidase of Gundelia tournefortii investigated at different pH, temperature and different inhibitors. Maximum activity of peroxidase achieved at pH 5.5-6 in according to type of substrate. Activity of peroxidase increased at constant concentration of H2O2 and different concentration of guaiacol, catechol and pyrogallol. The maximum activity in presence of 22mM of guaiacol was earned 2.4 Unit/mg.protein. At constant concentration of guaiacol and different concentration of H2O2, Vmax and Km were 2 Unit/mg.protein and 4 mM respectively. Activity decreased at high concentration of H2O2 so reached to 35% of Vmax at 40 mM. Catalytic efficiency of peroxidase in presence of constant concentrations of guaiacol and H2O2 calculated 0.5 and 0.5 Unit/mg protein mM-1 respectively. Stability of enzyme and maximum of activity was earned at 40 0C. Sodium cyanid and sodium azide showed inhibitory effect on activity with IC50 of 0.008 and 0.4 mM respectively. These results of inhibitors showed that inhibitory effect of Azid is 312 times of inhibitory effect of cyanide. Cyanid is competitive inhibitor of peroxidase in presence of catechol and pyrogallol and noncompetitive inhibitor of enzyme in presence of guaiacol, and Azide is noncompetitive inhibitor of peroxidase of root in gundelia tournefortii in presence of catechol and pyrogallol and uncompetitive inhibitor of enzyme in presence of guaiacol and kojic acid is uncompetitive inhibitor of peroxidase in presence of guaiacol and pyrogallol.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
1
11
https://eab.journals.pnu.ac.ir/article_3196_c850a3466c3b1cd263a9e0254d2f476e.pdf
Biogeochemical study of Pb element in native plants and domestic animals living on Irankoh mining area, Isfahan Province
Mehrnaz
Movahedi
Ph. D. Student of Biochemistry, Payame Noor University, Iran
author
Mehrdad
Movahedi
M.Sc. Student of Economic Geology, Geological Survey Employee, Tehran, Iran
author
text
article
2016
per
Heavy metals are the most important components of environment pollution. Soil contaminated by industrial and mining activities of these elements into the soil-plant-human cycle and accumulate in the human or animal body tissue to reach the levels of toxic for animals and humans. For this purpose, a broad sampling of soil, plant and sheep liver were taken and the amount of Pb were tested .Pb concentration was determined by atomic absorption spectrometry. The results indicated that the average concentration of heavy metals in soil, plants and sheep mining area is significantly higher than the control.Average Pb levels in the liver of sheep mine 4.5 μg/g is a threat to human health as the main consumer. Finally recommend taken sampling of water, soil, plant and animal tissue to control metal pollution develop speed in the environment and occurs necessary provisions
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
13
19
https://eab.journals.pnu.ac.ir/article_3197_a500b68bb7be2f48548b5d276d4de66b.pdf
Comparative histomorphology of cartilage tissue of vertebral column with dorsal fin in Persian sturgeon (Acipenser persicus)
Torab
Ghanbari
. Instructor, Department of Marine Biology, Faculty of Marine Science, Khoramshahr Marine Science and Technology University, Iran
author
Rahim
Abdi
Assistant Professor, Department of Marine Biology, Faculty of Marine Science, Khoramshahr Marine Science and Technology University, Iran
author
Mahmood
Bahmani
Associate Profesor, Department of Physiology and Histology, Dr Dadman International Sturgeon Research Institute of Rasht, Iran
author
Rezvanollah
Kazemi
Associate Profesor, Department of Physiology and Histology, Dr Dadman International Sturgeon Research Institute of Rasht, Iran
author
Ahmad
Savari
Professor, Department of Marine Biology, Faculty of Marine Science, Khoramshahr Marine Science and Technology University, Iran
author
text
article
2016
per
In this study cartilage tissue of vertebral column and dorsal fin of 40 days, 70 days, one and 4 years old of Persian sturgeon after preparation were fixed in buen fixative. Then rutin procedure of histologicql examination done and paraffin blocks cut at 6 microne and then stained with H&E, Safranin-O and Verhoff staining. In photomicrographs were compared cell structure and compounds of substance grounds in cartilage tissues. It showed that perichondrium layer of dorsal fin was more advanced from perichondrium layer of vertebral column and their mixture were different. Microscopic masurement of two cartilage tissue showed the cells of dorsal fin were larger than the cells of vertebral column but in cells number, decrised with physiological maturation. Comparative study of volume of extracellular matrix and number of isogenous groups by SPSS software showed that growth of cartilage tissue of vertebral column was interstitial but in dorsal fin was appositional.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
17
23
https://eab.journals.pnu.ac.ir/article_3198_5030a849ccf7de6f4ae90915a70217a1.pdf
Review stages of embryonic development stage hatching of the Sepia pharaonis
Meysam
Golzar
. MSc., Proliferation and aquaculture, Science and Research Branch of Tehran (Kurdistan), Sanandaj, Iran
author
Tooraj
Valinasab
. Professor, Iranian Fisheries Research Organization, Iran
author
Mehrdad
Shirin Abadi
Assistant Professor, Department of Fisheries, Islamic Azad University, Science and Research, Kurdistan, Sanandaj, Iran
author
Mahmood
Hafeziyeh
Associate Professor, Iranian Fisheries Research Organization, Iran
author
text
article
2016
per
The various stages of embryonic growth pharaoh´s cuttlefish (Sepia pharaonis) reside in Oman until hatching was studied. Gregor tours and the eggs collected for further study of the propagation of Education, Research Center of the Earth - Chabahar Moved .once every 24 hours to get detailed information was sampling of from fertilized eggs. Egg size of 11±4 mm were selected because of the large size of cuttlefish eggs during incubation stages of embryonic development within the capsule is visible. The studies showed that the water temperature of 20±2°C hatching takes about 20±10 days. In this study results showed that the first cleavage was performed on the first day and the 15th day after the yolk sac has started to decrease. Tentacle is visible on day 14. Cuttlefish in all developmental stages of the embryonic development of a model to follow, but the temperature plays an important role in hatching time.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
25
33
https://eab.journals.pnu.ac.ir/article_3199_b26fc05c8ce8a617df1e24449ace9fe0.pdf
Recent Advances in Cell therapy of spinal cord injury
Shiva
Nemati
Ph.D. Candidate in Biochemistry, Payame Noor University, Tehran Iran. And Researcher in Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
author
Ebrahim
Shahbazi
Ph.D. candidate in Developmental Biology, Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
author
Mehdi
Hesaraki
Ph.D. candidate in Developmental Biology, Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
author
Sahar
Kiani
Professor Asistant, Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
author
Reza
Haji Hossein
Professor in Biochemistry, Payame Noor university, Tehran, Iran
author
text
article
2016
per
Spinal cord injury (SCI) is a devastating condition producing great personal and societal costs and for which there is no effective treatment. Stem cell transplantation is a promising therapeutic strategy, though much preclinical and clinical research work remains. Here, we briefly describe SCI epidemiology, pathophysiology, and transplantation trial in human developments, including termination of the first human embryonic stem cell experimental and clinical stem cell strategies. Research in stem cell biology and cell reprogramming is rapidly advancing, with the hope of moving stem cell therapy closer to helping people with SCI. We examine issue important for clinical translation and provide a commentary on recent SCI.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
35
51
https://eab.journals.pnu.ac.ir/article_3200_8b51125f1342b582cf507ab4d80d4a44.pdf
Lizard biodiversity of Torbat-e Heydaryeh in Razavi Khorasan Province
Vajihe Sadat
Ghaemitalab
Master’s Graduate in Animal Science, Animal Bio-Systematic, Iran
author
Haji
Gholi Kami
. Assistant Professor, Department of Biology, Golestan University, Gorgan, Iran
author
text
article
2016
per
Squamata or scaled Reptiles are the largest order of new Reptiles including Lizards, Snakes and Amphisbaenia. They have formed 95 precent of new Reptiles. Our goal is Lizard Biodiversity of Torbat-e Heydaryeh in Razavi Khorasan Province. The study of Lizards Biodiversity of Torbat-e Heydaryeh in Razavi Khorasan Province was carried out in 2012 until 2013. In this research 132 specimens were collected and observed and identified on the basis of morphological, morphometric and meristic characters. The collected and observated Lizards belonging to 5 families: Agamidae, Lacertidae, Scincidae, Gekkonidae and Varanidae including 8 genera and 12 species. Results showed that Agamidae and Lacertidae families have the most frequency. The Agamidae family has the most genera and Lacertidae family has the most species. laudakia caucasia, laudakia microlepis, Trapelus sanguinolentus and Mesalina watsonana were the dominant species in this region and had a wider distribution rang in coparision with other species.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
53
66
https://eab.journals.pnu.ac.ir/article_3201_8989058406feb6de6a4f4648e304d638.pdf
Comparative study on Reproductive characteristics of Carassius gibelio (Bloch, 1782) in Boostan dam lake & Alakoli reservoir
Eisa
Hajiradkochak
. M.Sc. Student of aquatics Ecology, University of Gonbad Kavous, Gonbad Kavous, Iran
author
Rahman
Patimar
. Associate Professor Department of Fishery, Faculty of natural resource, University of Gonbad Kavous, Gonbad Kavous, Iran
author
Arsalan
Bahalkeh
M.Sc. of aquatics Ecology, Faculty of Natural Resource, University of Gonbad Kavous, University of Gonbad Kavous, Gonbad Kavous, Iran
author
text
article
2016
per
In this study were reviewed some biological characteristics including sex ratio, gonadosomatic index, fecundity and egg diameter Carassius gibelio in the study area from February to September 2015. During the study, 222 specimens from Boostan dam and 238 specimens from Alakoli Reservoir were sampled using small beach seine. The maximum total length and weight of Boostan dam for females and males respectively 206 mm, 141.12 gr and 126 mm, 33.90 gr and in the Alakoli Reservoir for females respectively 196 mm, 114.65 gr and for males was 190 mm, 98/64 gr. The sex ratio of fish caught from Boostan dam to 1:7.88 and the sex ratio of fish caught from the Alakoli Reservoir was 1: 10/90. The highest average gonadosomatic index (GSI) in Boostan dam for female in April (10.27±4.40) and the male (3.56±1.21) in March were observed, This factore in the Alakoli Reservoir area for female (11.73±2.99 ) and male(3.22±0.85) were observed on March and May. The Minimum, maximum and mean absolute fecundity in Boostan dam with 532/08 respectively, 82039/36 and 13119/44 and in the Alakoli pools area 1503/75, 120786/00 and 23174/60 and The minimum, maximum and average were Relative fecundity (eggs per kg body weight) respectively in Boostan dam with 37/54, 4344/34 and 593/10 and in the Alakoli reservoir respectively was 92/97, 23377/20 and 1987/60. The Eggs diameter of 0.11 to 1.00 mm and the average of 0.56 mm in Boostan dam lake area was variable. While this indicator of 0.11 to 1.07 and the average of 0.54 mm in Alakoli Reservoir area was variable.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
67
76
https://eab.journals.pnu.ac.ir/article_3202_6a858aeb9c888b7d8ce1d617c1c96f9f.pdf
Identification and abundance of hermit crabs (Crustacea: Paguroidea) in the southern of Qeshm Island; first record of Clibanarius virescens (Krauss, 1843) in the Persian Gulf
Nabiollah
Kheirabadi
Former M. Sc. Student, Marine Biology, Faculty of Marine Sciences, Tarbiat Modarres University, Iran
author
Sanaz
Stekani
M. Sc. Student, College of Marine Science, Chabahar Maritime University Marine Science, Iran
author
Elaheh
Darini
B. Sc. Student, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Iran
author
Rohollah
Kheirabadi
B. Sc. Student, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Iran
author
text
article
2016
per
The present work aims to determine fauna and abundance of hermit crabs in the southern Qeshm Island. For these aims, after preview of coastal zone, eight stations were selected in the intertidal zones of southern Qeshm Island. Sampling in these stations was conducted during the Dec. 2013. Totally, 605 specimens of hermit crabs were studied and calculated. For identification and accurate laboratory analysis, some of these specimens were fixed in 70% ethanol and transported to the laboratory. Base on valid identification keys, eight hermit crab species Clibanarius signatus, Clibanarius virescens, Diogenes avarus, Diogenes planimanus, Dardanus tinctor, Areopaguristes perspicax, Pagurus kulkarnii and Coenobita scaevola from six geneses and three families were identified in the intertidal zones of southern Qeshm Island. Also, Clibanarius virescens (Krauss, 1843), was reported in the Persian Gulf for the first time. All identified species were compared with the metatypical hermit crab samples in the Zoological Museum, University of Tehran and the species were verified. Compare the identified species in this study with the other studies showed that identified species in this study are so the same as the Oman Sea and north coast of Arabian Sea species. In addition, Station five (Mangrove Forest) had the highest rate of abundance of hermit crabs.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
77
86
https://eab.journals.pnu.ac.ir/article_3203_068718fcda4e0ea371970000e10c45c2.pdf
Assessment of morphometric and meristic characteristics of Garra rufa fish in the Dinawar River, Kermanshah Province
Mohammad
Javad Vesaghi
Ph.D. Student, Hormozgan University, Iran
author
Aliasghar
Khanipour
Associate Professor in National Inland Water Aquaculture institute Anzali, Iran
author
Masoud
Sattari
Department of Marine Sciences, The Caspian Sea Basin Research Center, University of Guilan, Iran Associate Professor in Department of Fishery, Faculty Natural Resources, University of Guilan, Iran
author
Mohammad
Forouhar Vajargah
Ph.D. Student of Aquatic Ecology, Faculty Natural Resources, University of Guilan, Iran
author
text
article
2016
per
This study carried out on 194 fish (Garra rufa) during the months of January to March at eight stations in Dinvar River located at Kermanshah province. During this study, 34 attributes and 11 traits count was measured geometric parameters and natural mortality and total amount 0.346, infinite length L∞=124.57 mm, growth factor K=0.29 and the length and weight of the exponential function of growth were calculated. The length-weight relationship was calculated for each season separately, which indicates isometric growth during summer and autumn and allometric growth during the winter and spring seasons. The length-weight relationship for the whole year was calculated aqual to W= 1/000095L2/97. Male to female sex ratio of 1: 1.44 were calculated for faster growth in males and natural selection is directed towards the elimination of larger samples. Results indicate that the Dinvar river is a good place for spawning and nursery to Brood fish. The data were confirmed by isolation of populations between the stations and it seems that fish populations of stations are panmictic. However, further studies confirm that these results depends on genetic traits, physiological and Osteology Lange populations.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
87
96
https://eab.journals.pnu.ac.ir/article_3204_4f8b9feef16db63258e5f7bb5189adc1.pdf
Quality Changes, Bacterial Community and Shel Life of Fish Fingers of Big-Head Carp Aristichthys nobilis during Storage at -18°C
Masoud
Hedayatifard
Associate Professor, Department of Fisheries, Advanced Educational Center, College of Agriculture, Qaemshahre Branch, Islamic Azad University, PO Box: 163, Iran
author
Negin
Rezaei
M.Sc. of Food Science and Technology Department, Ayatollah Amoli Branch, Islamic Azad University, Iran
author
text
article
2016
per
In this study, big-head carp flesh was processed and fish finger was produced by the formula of 1, 2 and 3 with 70, 80 and 93.5 percent of big head fish flesh respectively. Then sensory attributes, biochemical changes and nutritional value such as peroxide value (PV), thiobarbituric acid (TBA) and the total volatile nitrogen (TVB-N) changes and microbial load of all three formulas were compared with together. According to sensory data, the formula 1 was chosen as the selected product. Quality changes were studied for evaluators’ selected formula during storage at -18˚C on the days 0, 30, 60, and 90. The results showed that fish finger prepared with formula 1, had more acceptable scores than two other formulas. The results indicated that the minimum and maximum amounts of protein belonged to the formulas 1 and 2, respectively. The amount of protein, lipid, carbohydrate and calcium of the selected product decreased during the storage and the moisture had an increasing manner. The results of the study on the changes of PV, TBA and TVB-N showed that the minimum and maximum of PV belonged to formula 3 and formula 1, and the minimum and maximum of peroxide were observed on days 30 and 60 of storage, respectively. Microbial count showed that according to increasing storage time, total count was decreased. According to the results, maximum of shelf life for the samples stored at -18˚C was 90 days.
Experimental animal Biology
Payame Noor University
2322-2387
5
v.
2
no.
2016
97
109
https://eab.journals.pnu.ac.ir/article_3205_2d53fd7428109813398639c936ba6905.pdf