Zahra Adeli; Majid Rajabian; Hamid Sobhanian; Zahra Zamani
Abstract
Ovarian cancer happens as the result of change in gene and molecular metabolites. Because of chemotherapy side effects in cancer treatment such as drug resistance, using of complementary therapy with herbal and their derivatives is increased. This study evaluates anti-tumor effects of vitexin on AHRI, ...
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Ovarian cancer happens as the result of change in gene and molecular metabolites. Because of chemotherapy side effects in cancer treatment such as drug resistance, using of complementary therapy with herbal and their derivatives is increased. This study evaluates anti-tumor effects of vitexin on AHRI, p53 and Kras genes expression by Real time PCR. Also metabolite changes due to these variations are measured by 1D NOESY, 1 HNMR. SKOV3 cells treated whit different concentrations of vitexin and determined IC50 by MTT assay. The IC50 was measured as 520µg /ml. Then RNA extracting and building of cDNA done to determine level of genes expression changes. Metabolites extracted by water, chloroformed and methanol and lyophilized samples evaluated by 1HNMR. The expression of AHRI and p53 tumor suppressor genes in the treated cells increased by 1.93 and 1.76 times, respectively, and the expression of Kras oncogene gene decreased by 0.23 times. Maximum changes in metabolites pathways observed in Aminoacyl-tRNA biosynthesis, Biotin, cysteine, methionine, branch amino acids, lysine metabolism, and steroids biosynthesis. Vitexin shows its anti-tumor effects by targeting of several biochemical pathways and reload of metabolites by change in genes which have roll in ovarian cancer. So to confirm this study more evaluations in pathway signaling is needed.
