behrrouz heidari; Mina Ahmadpour; Razyeh Amani
Abstract
Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated ...
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Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health.
Fatemeh Kamranzadeh; Negin Salamat; Mohammad Ali Salari; , Abdolali Movahedinia
Abstract
Abstract The Persian Gulf is valuable marine ecosystem. The entrance of industrial waste waters especially from the petrochemical industries located in the cost line of the Persian Gulf resulted in considerable contamination of aquatics lives in this region. The present investigation aimed to study changes ...
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Abstract The Persian Gulf is valuable marine ecosystem. The entrance of industrial waste waters especially from the petrochemical industries located in the cost line of the Persian Gulf resulted in considerable contamination of aquatics lives in this region. The present investigation aimed to study changes in tissue structure of the liver in Otolithes ruber and Liza abu from the Musa creek, situated in the north west of the Persian Gulf, to evaluate the possible effects of environmental pollutants on these fish. In this study, 100 L. abu and O. ruber were collected from five sampling sites in Musa creek, including Petrochemical, Ghanam, Zangi, Douragh and Patil stations. Also, 10 fish/ species were collected from Sajafi creek as a control station. The samples were taken from the liver and were fixed in 15% formalin. Tissue samples were then processed using routine histological methods. Degeneration of cytoplasm, nucleus vacuolization, hemosiderin deposits and destroyed blood cells were the most tissue changes observed. The amount of Degree of Tissue Changes (DTC) for liver of both species was significantly higher and lower at the Petrochemical and Patil stations than other sites. The structural changes observed in both species showed close relation with the level of environmental pollution. In conclusion as it seems, the liver could be considered as an appropriate biomarker to assess the pollution of the region.
